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101.
MO Osman T El-Sefi SB Lausten NO Jacobsen CG Larsen SL Jensen 《Canadian Metallurgical Quarterly》1998,85(11):1487-1492
Previously, we described cloning of three alternatively spliced mRNA forms of human FGF8, a, b, and e, of which the b form is the major expressed species in both normal and tumor prostatic epithelial cells. In this report, we describe construction and overexpression of sense and antisense sequences of either the full length FGF8b coding region (215-amino acids or 215aa), 103aa N-terminal part or a smaller N-terminal region (34aa), each including the 23aa putative signal peptide domain, via a retrovirus system. While the morphologic transforming activities of the sense 215aa and 103aa constructs were similar in NIH3T3 cells, 103aa displayed reduced soft agar clonogenic activity. The 34aa construct was practically inert in these assays, although its expression could mimic the ability of 215aa or 103aa in conferring cell growth under reduced serum condition. Overexpression of any of the three constructs in antisense orientation, however, was similarly effective in reversing the morphology and anchorage-independent growth property of FGF8b-transfected NIH3T3 cells. The expression of the antisense 215aa construct significantly reduced the growth rate of the human prostatic carcinoma DU145 cells and inhibited their soft agar clonogenic activity and in vivo tumorigenicity in nude mice. Taken together, these results identify N-terminal portions of FGF8 protein isoform for having the domains necessary for one or more of the biologic effects examined, and suggest that low levels of FGF8 expressed in prostatic epithelial cells may contribute significantly to their growth and tumorigenic properties. 相似文献
102.
During a study of Digenea of shorebirds from Galveston, Texas, 30% (30 of 99) of willets, Catoptrophorus semipalmatus (Charadriiformes), were found to be infected with 55-405 Endocotyle bushi per infected host. Although the specimens from Texas generally agree with E. bushi from Florida, specimens from Texas are less elongated, have a more triangular to heart-shaped ovary, have slightly larger eggs, and have a smaller seminal vesicle. 相似文献
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Histocytic necrotizing lymphadenitis (HNL), also known as Kikuchi's disease is a benign self limiting disease of unknown origin. It effects cervical lymph nodes, predominantly in young females. It is well described in the pathology literature, but little is written about this in the ENT literature, as few clinicians are probably aware of this condition. Five cases of cervical Kikuchi's disease are presented with a brief review of the literature. 相似文献
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107.
Recovery of Amino Acids from Seafood Processing Wastewater with a Dual Chitosan-Based Ligand-Exchange System 总被引:1,自引:0,他引:1
Crawfish chitosan, prepared from crawfish shell chitin, was demonstrated to be an effective ligand-exchange column material for recovery of amino acids from seafood processing wastewater. In comparison with commercial chitosan, crawfish chitosan, loaded with copper or amino copper, showed higher recovery rates of amino acids. Recovery from amino copper-crawfish chitosan columns was pH dependent, with reduction at higher pH values. Eluatc was completely free of copper ions when treated with a second crawfish chitosan column. The amino acids recovered by this treatment have potential application as seafood flavors in terms of their sensory attributes. 相似文献
108.
Jin Young Choi Young Soo Kim Injin Sah Hee Cheon NO Changheui Jang 《International Journal of Hydrogen Energy》2014
The hydrogen iodide (HI) decomposition process is a limiting step for the efficiency of the sulfur–iodine nuclear hydrogen production process, owing to its low kinetics and complicated reaction characteristics. Therefore, the Korea Advanced Institute of Science and Technology (KAIST) suggested a simple high-temperature HI decomposition process at 650–700 °C for higher efficiency. For practical application of the high-temperature HI decomposition process, along with the catalyst study, we performed structure material selection and corrosion resistance tests. A number of candidate alloys were considered in various aspects and exposed to the high-temperature HI gas environment, which is extremely corrosive, at 850 °C for 100 h. Nine alloys with different nickel and iron compositions have been tested and analyzed. Test results indicated the degrees of resistances to corrosion of each alloy, on the basis of weight change and cross-sectional micrographs. Thus, because of their resistance to internal oxidation and formation of stable external oxide layers, five types of alloys, Haynes 214, aluminizing and inter-diffusion heat-treated or electron beam surface-treated Alloy 617 are suggested as appropriate candidates for fabricating high-temperature HI decomposer. In particular, surface-treatment of Alloy 617 gave it a high stability because of the resultant formation of an Al-rich layer; this was confirmed by experimental results, so IDHT Alloy 617 is recommended as a suitable structural material for fabricating HI decomposer. However, further long-term testing is suggested to ensure safety and confirm applicability. 相似文献
109.
恒定加速度试验的研究 总被引:1,自引:0,他引:1
对恒定加速度试验进行了研究,并对在试验中出现的一些问题进行了探讨。 相似文献
110.