Characterisation of a novel Drosophila melanogaster testis specific PP1 inhibitor related to mammalian inhibitor-2: identification of the site of interaction with PP1

Paralytic shellfish poisoning (PSP) toxins are produced by certain dinoflagellate species such as Gymnodinium catenatum and Alexandrium tamarensis, during certain periods of the year influenced by several environmental factors, affecting the aquaculture industry and mainly bivalve molluscs. HPLC with fluorescence detection is a powerful analytical technique for the analysis of such toxins; several HPLC alternatives have been developed in order to improve the liquid chromatographic analysis, but due to the complexity of the sample matrix, important work has been focused recently on the clean-up of samples prior to HPLC analysis. Solid-phase extraction procedures offer advantages for this clean-up. In this work we focus on the study of three different clean-up methods prior to HPLC with fluorescence detection analysis of PSP toxin present in contaminated mussel samples; by spiking uncontaminated mussel samples with two different PSP toxin standards and by calculating the recovery values for these experiments. These recoveries must be taken into account in order to quantify the exact amount of PSP toxins present in the contaminated samples.     

Serum concentrations of soluble adhesion molecules in patients with colorectal cancer

The concentrations of the soluble adhesion molecules E-cadherin, E-selectin, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were investigated in 48 patients with colorectal cancer before treatment, and their relation to clinical, histological and routine laboratory parameters was examined. Data were collected on tumour stage at presentation, presence and sites of metastatic disease, tumour pathology and results of routine laboratory tests. Serum concentrations of ICAM-1 and VCAM-1 were significantly elevated in the patients with colorectal cancer in comparison with a group of healthy subjects (P < 0.00001). Levels of circulating ICAM-1 and VCAM-1 were increased both in patients with local and those with metastatic disease. Although elevated in some patients soluble E-cadherin and E-selectin concentrations were not significantly elevated compared with the control group (P = 0.71 and P = 0.052 respectively). The levels of circulating ICAM-1 were significantly correlated with those of VCAM-1 and E-selectin. A correlation was also found between the serum concentrations of E-selectin and ICAM-1 and alkaline phosphatase, total white cell count and platelet count. VCAM-1 was positively correlated with age and negatively with degree of tumour differentiation and haemoglobin concentration. The biological implications and possible clinical relevance of these findings are discussed.     

Pattern of crack formation in rice grain as influenced by shape and orientation of cells

Stress cracks usually develop across the grain in rice, although longitudinal cracks also develop occasionally on one axis. On examination of sections of rice grain it was noticed that orientation of elongated cells provided cell wall continuation across the grain and also along the length in the dorsiventral axis. Cracks were found to develop easily along these cell wall lines. Crack formation was difficult in the lateral axis along the length since cell wall continuity was absent. However, when severely dried grains were suddenly wetted cracks developed even in this axis by disruption of cells along the cleavage path.     

The role of estrogen and progesterone in the regulation of reproductive behaviour in female ring doves(Streptopelia risoria) under long vs. short photoperiods

The role of estrogen and progesterone in the regulation of reproductive behavior was studied in female ring doves (Streptopelia risoria) under long vs. short photoperiods. The doves held under long (16 hours/day) or short (8 hours/day) photoperiods were treated for 15 days with saline, estrogen, or progesterone, alone or in combination. 7 days after the start of hormone therapy, the doves were placed with reproductively active males for 4 hours/day for 9 days. No difference was seen in egg-laying, courtship or nest-building by control birds under the 2 photo regimes. Ovarian follicles remained small in all doves receiving hormone treatment. Oviducts of doves receiving progesterone alone remained small in the short-photoperiod group but at long photoperiods the oviducts became enlarged. Estrogen stimulated oviduct growth at both photoperiods wheras the combined hormone treatment resulted in greater oviduct development than estrogen alone (p less than .02). Female soliciting and nest-building activity remained low in progesterone-treated doves under short photoperiod but increased rapidly under long photoperiod. Doves treated with estrogen and estrogen plus progesterone performed considerable soliciting and nest-building. No marked tendency was seen for doves under long-photoperiod conditions to be more active in nest-oriented behavior. Copulatory behavior by the female occurred infrequently in all hormone-treated doves.