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61.
62.
Improved texture analysis for hydrogel characterization: Gel cohesiveness,adhesiveness, and hardness
Julia Hurler André Engesland Bahador Poorahmary Kermany Nataša Škalko-Basnet 《应用聚合物科学杂志》2012,125(1):180-188
The texture properties of formulation are an important parameter in optimization of topical formulations. These properties will affect applicability of the formulation at the administration site and therapy outcome. Our aim was to develop a fast and reliable method to characterize texture properties of hydrogels, namely cohesiveness, adhesiveness, and hardness. During the method development, we realized that the measurements setup needed to be adjusted for each hydrogel type, namely Carbopol, chitosan, and poloxamer hydrogels. The influence of the polymer concentration, pH, and incorporation of additives such as glycerol, drug solution, or liposomes on the texture properties, as determined by Texture Analyzer, was evaluated. In addition, the new method was applied to determine the changes during the accelerated stability testing. While Carbopol and poloxamer gels showed a linear relationship between the polymer concentration and texture properties, for low molecular weight chitosan gels the properties increased in exponential manner with increasing polymer concentration. The effect of incorporated liposomes on the gel properties was found to be dependent on the type of hydrogel. The hydrogel hardness was affected by the temperature as seen in accelerated stability testing. The method represents a valuable tool in pharmaceutical and cosmetics formulation development. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012 相似文献
63.
Jasna Bertoncelj Tomaž Polak Tina Pucihar Nataša Lilek Andreja Kandolf Borovšak Mojca Korošec 《International Journal of Food Science & Technology》2018,53(8):1880-1888
Bee pollen is a source of nutrients that are important for humans. There is growing interest in bee pollen, mainly due to consumers wishing to use natural products for a healthy diet or for their therapeutic effects. The composition of bee pollen varies according to botanical and geographical origin. The aim was to define for the first time the carbohydrate composition of bee pollen from Slovenia. A total of twenty‐eight samples of bee pollens were analysed for botanical origins and contents of water, sugars and soluble and insoluble dietary fibre. From the bee pollen samples analysed, ten were recognised as monofloral. Monosaccharides represented 96% of the sugar fraction, with ranges 13.2–27.8 g per 100 g dry weight for fructose and 10.6–28.5 g per 100 g dry weight for glucose. Levels of sucrose, maltose and melezitose were low. Total dietary fibre was 10.0–21.4 g per 100 g dry weight bee pollen, with 73–82% insoluble fibre. Bee pollen can thus provide a good source of dietary fibre. This study supports further nutritional proposals for Slovenian bee pollen. 相似文献
64.
The expression and activity of antioxidant enzymes in the liver of rats exposed to high‐fructose diet in the period from weaning to adulthood 下载免费PDF全文
65.
Impact of ultrasound on egg white proteins as a pretreatment for functional hydrolysates production 总被引:1,自引:0,他引:1
Andrea B. Stefanović Jelena R. Jovanović Sanja Ž. Grbavčić Nataša Ž. Šekuljica Verica B. Manojlović Branko M. Bugarski Zorica D. Knežević-Jugović 《European Food Research and Technology》2014,239(6):979-993
The objective of this study was to investigate the effects of different ultrasound pretreatment on enzymatic hydrolysis of egg white proteins (EWPs) by Alcalase as well as evaluating some functional and antioxidant properties of hydrolysates obtained by various proteases treatment and ultrasound technology. The effects of chosen ultrasound pretreatment parameters including frequency of ultrasonic waves (35 and 40 kHz), temperature (25 and 55 °C), time of pretreatment (15–60 min) and pH of egg white solution (7.00–10.00) were examined. It appeared that controlled ultrasound treatment can improved the hydrolysis process compared with untreated samples, but optimization of the power and length of sonication was important. The optimal ultrasound pretreatment at calorimetric power of 21.3 W and frequency of 40 kHz for 15 min at 25 °C and with naturally basic egg white (pH 9.25) resulted in increased initial rate and equilibrium degree of Alcalase hydrolysis by about 139.8 and 13.86 % compared with the control, respectively. EWP hydrolysates with ≈27.0 % degree of hydrolysis obtained with heat pretreatment and ultrasound pretreatments under optimal conditions were further separated by sequential ultrafiltration into 4 hydrolysate fractions (<1, 1–10, 10–30 and >30 kDa) which were investigated for protein content, peptide yield and antioxidant activity. The hydrolysis after heat pretreatment generated more peptides <1 kDa (19.04 ± 1.02 %) than ultrasound pretreatment did (11.90 ± 0.53 %), whereas the proportion of peptides <10 kDa were higher in the second case (28.80 ± 0.07 vs. 20.46 ± 0.39 %). The fraction obtained by the ultrasound pretreatment containing peptides with a molecular weight between 1 and 10 kDa demonstrated the strongest ABTS radical scavenging efficacy among the fractions (97.54 ± 0.30) with IC50 value of 4.31 mg/mL. Compared with single-enzyme processes, the two-stage enzymatic processes did not significantly improve both antioxidant and functional hydrolysates’ properties. 相似文献
66.
It has been shown theoretically that three dimensional graph structure and DNA self-assembly can be used to solve numerous
computational problems such as 3-SAT and 3-colorability in a constant number of laboratory steps. In this assembly, junction
molecules and duplex DNA molecules are the basic building blocks. This paper presents experimental results of DNA self-assembly
of non regular graphs using junction molecules as vertices and duplex DNA molecules as edge connections. 相似文献
67.
68.
Slavica Maletić Nataša Jović Orsini Mirjana Milić Jablan Dojčilović Amelia Montone 《应用聚合物科学杂志》2024,141(5):e54881
Epoxy-rich carbon-based composites are well recognized materials in industries owing to their good mechanical properties and thermal stability. Here, dielectric properties of composites based on bisphenol-A-epoxy resin loaded with 5, 6, 10, and 15 wt% of graphite flakes (GF) have been studied. The frequency and temperature dependence of the dielectric permittivity, dielectric loss, and ac conductivity have been examined in temperature (−103 to 97°C) and frequency (20 Hz–200 kHz) range. Influence of the filler surface chemistry have been studied for composites loaded with 5 wt% GF obtained: (i) under wet milling, without or with adding Triton-100x as a surfactant, or (ii) under dry milling in the presence of KOH. The composite made of epoxy loaded with 5 wt% exfoliated expanded graphite flakes (EEG), was also prepared. The surface treatment with KOH notably increased dielectric constant of the composite, keeping low dielectric loss, while treatment with Triton-100x significantly increased tanδ. The composite loaded with exfoliated expanded graphite shows higher ac conductivity than those obtained with flaky graphite, GF. Possibility to change dielectric properties of the composites without changing the loading content can be used as an approach in tailoring one with desired dielectric properties. 相似文献
69.
Ascorbic acid (AA) was encapsulated into dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylcholine/cholesterol (DPPC/chol) liposomes. We have shown that co-encapsulation of citric and ascorbic acids into liposomes results in considerable stabilization of the latter in model systems containing catalytic concentrations of copper ions. The rate of AA oxidation was decreased by up to 300-fold that of free AA. Incorporation of cholesterol into membranes resulted in lower stabilization efficacy at room temperature and better thermal stability at higher temperatures than pure DPPC liposomes. Total AA, extra-liposomal AA and leakage of AA out of liposomes during thermal treatment were evaluated using the free radical 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS). A modification of the ABTS based assay for liposome permeability makes it applicable to reducing agents and antioxidants as well as AA. Encapsulated AA was also stabilized in real food matrices. The rate of AA oxidation in apple juice, relative to that of free AA, was decreased by two orders of magnitude when encapsulated in DPPC liposomes and by more than one order of magnitude in DPPC/chol liposomes. Liposomal stabilization of AA in a fermented milk product was less pronounced. 相似文献
70.
Nataša Poklar Ulrih 《Critical reviews in food science and nutrition》2017,57(10):2144-2161
This mini review focuses on advances in biophysical techniques to study polyphenol interactions with proteins. Polyphenols have many beneficial pharmacological properties, as a result of which they have been the subject of intensive studies. The most conventional techniques described here can be divided into three groups: (i) methods used for screening (in-situ methods); (ii) methods used to gain insight into the mechanisms of polyphenol–protein interactions; and (iii) methods used to study protein aggregation and precipitation. All of these methods used to study polyphenol–protein interactions are based on modifications to the physicochemical properties of the polyphenols or proteins after binding/complex formation in solution. To date, numerous review articles have been published in the field of polyphenols. This review will give a brief insight in computational methods and biosensors and cell-based methods, spectroscopic methods including fluorescence emission, UV-vis adsorption, circular dichroism, Fourier transform infrared and mass spectrometry, nuclear magnetic resonance, X-ray diffraction, and light scattering techniques including small-angle X-ray scattering and small-angle neutron scattering, and calorimetric techniques (isothermal titration calorimetry and differential scanning calorimetry), microscopy, the techniques which have been successfully used for polyphenol–protein interactions. At the end the new methods based on single molecule detection with high potential to study polyphenol–protein interactions will be presented. The advantages and disadvantages of each technique will be discussed as well as the thermodynamic, kinetic or structural parameters, which can be obtained. The other relevant biophysical experimental techniques that have proven to be valuable, such electrochemical methods, hydrodynamic techniques and chromatographic techniques will not be described here. 相似文献