Stabilization of Glucagon by Trehalose Glycopolymer Nanogels

Glucagon is a peptide hormone used for the treatment of hypoglycemia; however, its clinical potential is limited by its insolubility and instability in solution. Herein, the encapsulation, stabilization, and release of glucagon by trehalose glycopolymer nanogels are reported. Methacrylate‐functionalized trehalose is copolymerized with pyridyl disulfide ethyl methacrylate using free radical polymerization conditions to form trehalose glycopolymers with thiol‐reactive handles. Glucagon is chemically modified to contain two thiol groups and is subsequently utilized as the cross‐linker to form redox‐responsive trehalose nanogels with greater than 80% conjugation yield. Nanogel formation and subsequent glucagon stabilization are characterized using polyacrylamide gel electrophoresis, dynamic light scattering, and transmission electron microscopy. It is determined that the solution stability of the glucagon increased from less than 24 h to at least three weeks in the nanogel form. Additionally, in vitro activity of the synthesized glucagon analog and released glucagon is investigated, demonstrating that the glucagon remains active after modification. It is anticipated that these glucagon–nanogel conjugates will be useful as a stabilizing glucagon formulation, allowing for cargo release under mild reducing conditions.     

Autonomic Self‐Healing of PEDOT:PSS Achieved Via Polyethylene Glycol Addition

Self‐healing electronic materials are of primary interest for bioelectronics and sustainable electronics. In this work, autonomic self‐healing of films obtained from mixtures of the conducting polymer poly(3,4‐ethylenedioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS) and polyethylene glycol (PEG) is reported. The presence of PEG in PEDOT:PSS films decreases the elastic modulus and increases the elongation at break, thus leading to a softer material with enhanced self‐healing characteristics. In situ imaging of the cutting/healing process shows that the healing mechanism is likely due to flowing back of the material to the damaged area right after the cutting.     

Changing Deliberative Norms on News Organizations' Facebook Sites

Comments posted to news sites do not always live up to the ideals of deliberative theorists. Drawing from theories about deliberation and group norms, this study investigates whether news organizations can affect comment section norms by engaging directly with commenters. We conducted a field study with a local television station in a top‐50 Designated Market Area. For 70 political posts made on different days, we randomized whether an unidentified staff member from the station, a recognizable political reporter, or no one engaged with commenters. We assessed if these changes affected whether the comments (n = 2,403) were civil, were relevant, contained genuine questions, and provided evidence. The findings indicate that a news organization can affect the deliberative behavior of commenters.     

The importance of native panel contrast and local dimming density on perceived image quality of high dynamic range displays

We evaluated the perceived image quality of High Dynamic Range (HDR) content rendered using different types of local dimming and organic light‐emitting diode (OLED) displays. Using an OLED display that is capable of achieving high contrast at a pixel level, we emulated local dimming displays to evaluate their image quality. In a set of subjective experiments, observers compared HDR images and videos rendered with different local dimming densities and native panel contrast. There was a strong effect of panel contrast on perceived quality and also a strong trend toward preference for a larger number of dimming zones. We also evaluated the panel contrast and number of local dimming zones necessary to achieve image quality comparable with OLED. The findings of these experiments demonstrated that the use of a high‐contrast panel remains of critical importance. Also, the preference for panel rendering mode remains robust to normal levels of indoor ambient light.     

Restarting automata with auxiliary symbols restricted by lookahead size

This paper presents a study on lookahead hierarchies for restarting automata with auxiliary symbols. We show that the class of languages for deterministic monotone or monotone restarting automaton, whose restart step and rewrite step are separated, coincides with that of the same type of restarting automaton whose restart and rewrite steps are not separated, for any fixed lookahead size. For the non-monotone deterministic case, the lookahead length must be approximately doubled. We then turn our attention to restarting automata with small lookahead. For the general restarting automaton model, we show that there are just two different classes of languages recognized, through the restriction of lookahead size: those with lookahead size 1 and those with lookahead size 2. We also show that the respective (left-) monotone restarting automaton models characterize the context-free languages and that the respective right–left-monotone restarting automata characterize the linear languages both with just lookahead length 2.     

Quasi‐isothermal DSC testing of epoxy adhesives using initial fast heating rates

Three major factors decrease the accuracy of the cure measurement in standard‐isothermal testing using differential scanning calorimetry (DSC). First, cure occurs during the heating step. Second, data are lost during the stabilization period between the dynamic and isothermal step. Third, the baseline selection requires a modification to the protocol. An alternative, which is explored in this study, is the use of fast ramps, which decrease the heating time, but this has been avoided due to overshoot that occurs between the dynamic and isothermal step, which is troublesome for systems with autocatalytic kinetics. By mitigating these factors, a quasi‐isothermal protocol was developed. Therefore, more complete cure kinetics were captured with the implementation of fast DSC to decrease the ramp time and through the optimization of furnace parameters to decrease stabilization time and temperature overshoot. The data suggested this quasi‐isothermal analysis more accurately measured the isothermal curing kinetics of a commercial epoxy adhesive at 110, 115, and 120 °C for fast ramps of 175, 350, and 500 K/min compared to the traditional ramp of 5 K/min. The enthalpy spike at the dynamic to isothermal transition remains an issue; however, an empirical shift can be used to compensate for the enthalpy signal lag. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134, 45425.     

Comparison of direct electrocaloric characterization methods exemplified by 0.92 Pb(Mg1/3Nb2/3)O3‐0.08 PbTiO3 multilayer ceramics

Electrocaloric device structures have been developed as multilayer ceramics (MLCs) based on fundamental research carried out on PMN‐8PT bulk ceramics. Two different MLC structures were prepared with nine layers each and layer thicknesses of 86 μm and 39 μm. The influence of the device design on its properties has been characterized by microstructural, dielectric, ferroelectric, and direct electrocaloric measurement. For direct characterization two different methods, ie temperature reading (thermistor and thermocouple) and heat flow measurement (differential scanning calorimetry), were used. A comparison of results revealed a highly satisfactory agreement between the different methods. This study confirms that MLCs are promising candidates for implementation into energy‐efficient electrocaloric cooling systems providing large refrigerant volume and high electrocaloric effect. Due to their micron‐sized active layers, they allow for the application of high electric fields under low operation voltages. We measured a maximum electrocaloric temperature change of ΔT=2.67 K under application/withdrawal of an electric field of ΔE=16 kV mm^?1, which corresponds to operation voltages below 1.5 kV.     

Soil CO<Subscript>2</Subscript>, CH<Subscript>4</Subscript>, and N<Subscript>2</Subscript>O fluxes over and between tile drains on corn,soybean, and forage fields under tile drainage management

Controlled tile drainage (CTD) can benefit the environment and crop production. However, CTD has the potential to increase soil greenhouse gas (GHG: CO₂, CH₄, N₂O) emissions by increasing soil water contents and elevating field water levels. A paired-field (CTD and uncontrolled tile drainage (UTD)) approach was used to compare soil GHG emissions for silt loam corn, soybean, and forage fields under CTD and UTD management in eastern Ontario, Canada during a drier and a wetter growing season. A total of five field pairs were examined. Soil GHG emissions directly over tile drains (OT) and between tile drains (BT) in the CTD fields were also assessed. Average soil GHG emissions did not significantly differ (p > 0.05) for CTD and UTD field pairs, except for CO₂ emissions (greater emissions from UTD fields) among two field pairs studied (forage in the drier growing season and soybean in the wetter growing season), and N₂O emissions from a soybean field pair in the wet growing season (greater emissions from CTD field). Significantly higher soil water contents in the UTD forage field may have augmented CO₂ fluxes there. There were some significantly higher N₂O (in the wetter growing season) and CO₂ emissions (in both growing seasons) BT relative to OT locations in some fields; but these differences were not translated significantly to other BT and OT site comparisons. The wetter growing season examined resulted in greater average daily soil CO₂ fluxes overall, but similar CH₄ and N₂O fluxes for soybean fields compared to soybean fields in the drier growing season. Overall, there were no spatially or temporally systematic differences in GHG emissions among CTD and UTD field pairs, or among BT and OT locations in CTD fields.     

An Economic Evaluation Of Contracting Out And Competitive Tendering In Australian Local Government

Competitive tendering and contracting out have been adopted throughout the developed world as a mechanism to enhance public sector efficiency, and now form an important part of local government reform programs in Australia. This paper seeks to review competitive tendering and contracting out in Australian local government. It examines the meaning of the terms competitive tendering and contracting out, the development of these instruments in Australia, their economic characteristics, and empirical evidence on the efficacy of competitive tendering and contract out.