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71.
We have examined the interactions of various peptides with the mouse class II major histocompatibility complex molecule I-Ak. The peptides were derived from the model protein hen egg white lysozyme (HEL). The immunodominant peptide of HEL is a 10-mer, residues 52-61. Our previous work established that this sequence contains the key residues for binding and presentation to T cells. Now we show that the binding of this 10-mer sequence resulted in complexes of I-Ak and peptide that, in SDS/PAGE (without boiling the protein), rapidly dissociated from the component alpha and beta chains. The binding interactions were studied in vitro, by incubating purified I-Ak and radiolabeled peptide, or ex vivo, by using antigen-presenting cells incubated with peptides. Peptides with additional residues at either the amino or carboxyl terminus behaved dramatically differently. Complexes of I-Ak with the longer peptides were stable to SDS/PAGE. Very few amino acid additions result in the change from unstable to stable complexes. The important issue here is that when cultured with HEL, antigen-presenting cells selected the HEL peptides containing the 52-61 sequences that favored stability [Nelson, C. A., Roof, R. W., McCourt, D. W. & Unanue, E. R. (1992) Proc. Natl., Acad. Sci. USA 89, 7380-7383]. Also, from other studies, such sequences correlate with a high immunogenicity of the peptide. We conclude that there are structural features of peptides that change the stability of the class II molecule and that are independent of the "core" peptide seen by the T cells. 相似文献
72.
Sanabria NR Peralta YM Monta?ez MK Rodríguez-Valencia N Molina R Moreno S 《Water science and technology》2012,66(8):1663-1668
The effluent from the anaerobic biological treatment of coffee wet processing wastewater (CWPW) contains a non-biodegradable compound that must be treated before it is discharged into a water source. In this paper, the wet hydrogen peroxide catalytic oxidation (WHPCO) process using Al-Ce-Fe-PILC catalysts was researched as a post-treatment system for CWPW and tested in a semi-batch reactor at atmospheric pressure and 25 °C. The Al-Ce-Fe-PILC achieved a high conversion rate of total phenolic compounds (70%) and mineralization to CO(2) (50%) after 5 h reaction time. The chemical oxygen demand (COD) of coffee processing wastewater after wet hydrogen peroxide catalytic oxidation was reduced in 66%. The combination of the two treatment methods, biological (developed by Cenicafé) and catalytic oxidation with Al-Ce-Fe-PILC, achieved a 97% reduction of COD in CWPW. Therefore, the WHPCO using Al-Ce-Fe-PILC catalysts is a viable alternative for the post-treatment of coffee processing wastewater. 相似文献
73.
The consequences of a vortex unbinding picture of two-dimensional superconductivity are worked out. Although there is no true finite-temperature phase transition, dirty superconducting films should display anomalous behavior below the BCS transition temperature and above an effective Kosterlitz-Thouless vortex unbinding temperature. In particular, both the conductivity and fluctuation diamagnetism behave like
+
2
in this regime, where + is the correlation length calculated by Kosterlitz, +
c
exp (B/T – T
c)1/2. We estimate c, B, and the vortex unbinding temperature, and determine the nonlinear resistivity below T
c. A recent theory of vortex dynamics, together with a time-dependent Ginzburg-Landau theory, lead to a determination of the frequency-dependent conductivity.This work was supported by the National Science Foundation under Grant No. DMR 77-10210. 相似文献
74.
75.
The multigrounded neutral system for power systems above 600 V is a reasonable and safe design. The multigrounded neutral system provides 1) safety, 2) low zero sequence impedance than the single-point grounded neutral system, 3) lightning-arrester sizes can be optimized, 4) freezing and arctic conditions have an adverse impact on the zero sequence impedance, 5) cost of equipment for the multigrounded system is lower. 相似文献
76.
Transformer oil/cellulose structures are often designed based on a cumulative stress criterion derived from experimental tests at power frequency. However, such structures must also meet stringent impulse requirements defined by a basic insulation level (BIL). The industry has tried to establish an equivalence factor to permit power frequency cumulative stress methods to be used to estimate impulse withstand strength. Since the mechanisms of failure differ substantially under surge conditions, there would seem no good reason to suppose that a universal equivalence factor is appropriate. Tests are reported using a 2.3 MV generator to document impulse failure of a number of bulk, creep and hybrid structures to establish the nature of this relationship through statistical comparisons with the established 50/60 Hz methods. Factors varied from 1.94 to 3.34, depending on the configuration. The methodology is described and the results discussed in the context of the design of oil-cellulose structures, having regard to complicating factors such as waveshape and electrode covering. The study permits some speculation about impulse design under hybrid situations (i.e. failure paths involving both creep and bulk liquid). 相似文献
77.
We present a method for autonomous learning of dextrous manipulation skills with multifingered robot hands. We use heuristics derived from observations made on human hands to reduce the degrees of freedom of the task and make learning tractable. Our approach consists of learning and storing a few basic manipulation primitives for a few prototypical objects and then using an associative memory to obtain the required parameters for new objects and/or manipulations. The parameter space of the robot is searched using a modified version of the evolution strategy, which is robust to the noise normally present in real-world complex robotic tasks. Given the difficulty of modeling and simulating accurately the interactions of multiple fingers and an object, and to ensure that the learned skills are applicable in the real world, our system does not rely on simulation; all the experimentation is performed by a physical robot, in this case the 16-degree-of-freedom Utah/MIT hand. Experimental results show that accurate dextrous manipulation skills can be learned by the robot in a short period of time. We also show the application of the learned primitives to perform an assembly task and how the primitives generalize to objects that are different from those used during the learning phase. 相似文献
78.
López Steven Regeser; Nelson Hipke Kathleen; Polo Antonio J.; Jenkins Janis H.; Karno Marvin; Vaughn Christine; Snyder Karen S. 《Canadian Metallurgical Quarterly》2004,113(3):428
The authors examined the role of family factors and the course of schizophrenia by carrying out additional assessments and analyses in 2 previously published studies of Mexican American and Anglo American patients and families. The authors found partial support for an attributional model of relapse for families who are low in emotional overinvolvement. Attributions of control, criticism, and warmth together marginally predicted relapse. The data also indicated that for Mexican Americans, family warmth is a significant protective factor, whereas for Anglo Americans, family criticism is a significant risk factor. These findings suggest that the sociocultural context shapes the pathways by which family processes are related to the course of illness. Moreover, the warmth findings suggest that families may contribute to preventing relapse. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
79.
Pedersoli E Capotondi F Cocco D Zangrando M Kaulich B Menk RH Locatelli A Mentes TO Spezzani C Sandrin G Bacescu DM Kiskinova M Bajt S Barthelmess M Barty A Schulz J Gumprecht L Chapman HN Nelson AJ Frank M Pivovaroff MJ Woods BW Bogan MJ Hajdu J 《The Review of scientific instruments》2011,82(4):043711
We present a compact modular apparatus with a flexible design that will be operated at the DiProI beamline of the Fermi@Elettra free electron laser (FEL) for performing static and time-resolved coherent diffraction imaging experiments, taking advantage of the full coherence and variable polarization of the short seeded FEL pulses. The apparatus has been assembled and the potential of the experimental setup is demonstrated by commissioning tests with coherent synchrotron radiation. This multipurpose experimental station will be open to general users after installation at the Fermi@Elettra free electron laser in 2011. 相似文献
80.
Nelson James Byron; Sanjuan María del Carmen; Vadillo-Ruiz Sandra; Pérez Joana; León Samuel P. 《Canadian Metallurgical Quarterly》2011,37(1):58
Two experiments with human participants are presented that differentiate renewal from other behavioral effects that can produce a response after extinction. Participants played a video game and learned to suppress their behavior when sensor stimuli predicted an attack. Contexts (A, B, & C) were provided by fictitious galaxies where the game play took place. In Experiment 1, participants who received conditioning in A, extinction in B, and testing in A showed some context specificity of conditioning during extinction and a recovery of suppression on test. Experiment 2 demonstrated recovery of extinguished responding when participants were conditioned in A, extinguished in B, and tested in C, a third, neutral context. The experiment also demonstrated that the context of extinction did not control performance by becoming inhibitory. Results are discussed in terms of mechanisms that can produce a response recovery after extinction. The experiments demonstrated a renewal effect: a response recovery that was not attributable to the contexts acting as simple conditioned stimuli and is the first work with human participants to conclusively do so. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献