Occurrence of human papillomavirus infection in cervical intraepithelial neoplasia. A retrospective histopathological study of 317 cases treated by laser conization

The aim of the study was to compare the histological outcome of the cone specimens with the diagnoses of the preoperative biopsies, to assess the distribution of histological features consistent with human papillomavirus (HPV) infection and, finally, to analyse the impact of cellular HPV features on classification of cervical intraepithelial neoplasia (CIN). The study comprised a population of 317 women treated for CIN by laser conization during the period 1983-85. A total of 634 cervical specimens (317 preoperative biopsies and their corresponding cones) were studied retrospectively for CIN classification and examined for morphological signs of HPV infection. For presentation of the results, we used a modified terminology for CIN. Low-grade (LG) CIN included borderline lesions and CIN I, while high-grade (HG) CIN included CIN II and CIN III. The blinded histopathological review revealed HG CIN both in the preoperative biopsies and the cones in 71% of the cases. LG CIN or benign lesions were found in the preoperative biopsies and their corresponding cone specimens in 6% of the study population. HPV features were present in 65% of the preoperative biopsies, and were most prevalent in women under 29 years of age (p < 0.001). Thirteen percent of the total biopsy material was downgraded. The downgrading was most prevalent among original CIN II (p = 0.009) and HPV-negative biopsies (p < 0.001). This study demonstrates that CIN lesions are frequently associated with HPV features, which are significantly more prevalent in the youngest women. Concomitant HPV features do not influence the CIN classification.     

Modification of method for determination of medium-molecular peptides and its application in pediatric surgery

Taking into account clinical significance of determination of medium-molecular peptides' concentration in various surgical diseases and limitation of blood sampling in children, modification of the method for determination of medium-molecular peptides in blood serum is proposed, which requires 0.1 ml of serum and is based on deproteinization of cooled ethanol followed by spectrophotometry of peptide solution at 210 nm. Comparison study of the results of examination by this method and routine one was carried out. Specificity and advantages of the suggested modification are discussed.     

Dupuytren''s disease--a study of the pattern of distribution and stage of contracture in the hand

A clinical surface electrocardiogram (ECG) in which ventricular reciprocal rhythm occurred occasionally three times and probably seven times in succession together with paroxysmal ventricular tachycardia and ventricular extrasystoles in competition with sinus rhythm causing incomplete A-V dissociation is described. An ECG tracing of ventricular reciprocal rhythm occurring in succession has never been reported previously as far as can be ascertained from published reports.     

Controlled reperfusion of cardiac grafts from non-heart-beating donors

BACKGROUND: Hearts harvested from non-heart-beating donors sustain severe injury during procurement and implantation, mandating interventions to preserve their function. We tested the hypothesis that limiting oxygen delivery during initial reperfusion of such hearts would reduce free-radical injury. METHODS: Rabbits sustained hypoxic arrest after ventilatory withdrawal, followed by 20 minutes of in vivo ischemia. Hearts were excised and reperfused with blood under conditions of high arterial oxygen tension (PaO2) (approximately 400 mm Hg), low PaO2 (approximately 60 to 70 mm Hg), high pressure (80 mm Hg), and low pressure (40 mm Hg), with or without free-radical scavenger infusion. Non-heart-beating donor groups were defined by the initial reperfusion conditions: high PaO2/ high pressure (n = 8), low PaO2/high pressure (n = 7), high PaO2/low pressure (n = 8), low PaO2/low pressure (n = 7), and high PaO2/high pressure/free-radical scavenger infusion (n = 7). RESULTS: After 45 minutes of reperfusion, low PaO2/ high pressure and high PaO2/low pressure had a significantly higher left ventricular developed pressure (63.6 +/- 5.6 and 63.1 +/- 5.6 mm Hg, respectively) than high PaO2/high pressure (40.9 +/- 4.5 mm Hg; p < 0.0000001 versus both). However, high PaO2/high pressure/free-radical scavenger infusion displayed only a trend toward improved ventricular recovery compared with high PaO2/ high pressure. CONCLUSIONS: Initially reperfusing nonbeating cardiac grafts at low PaO2 or low pressure improves recovery, but may involve mechanisms other than decreased free-radical injury.     

Carbohydrate adaptive substance for prophylaxis of immune disorders and correction of dysbacterioses

Effects of a new preparation, adaptokhit, on the intestinal microbiocenosis and some parameters of immune resistance were studied in Macaque rhesus during 13-day hypokinesia. Experimental animals were given adaptokhit as an alimentary supplement preventing dysbiotic consequences of the microecologic unbalance due to motor restraint. Results of the investigation showed certain distinctions in the intestinal microflora in the experimental and control animals as early as on day three of the experiment. Intestinal microflora of the experimental primates who daily consumed adaptokhit at 50 mg/kg of the body remained stable throughout the period of observation. In contrast, in their controls lactoflora was found to decrease in parallel to the growth of opportunistically pathogenic endobacteria including representatives of Proteus and Clostridia sp., and development of dysbacteriosis of categories II and III. Besides, in the experimental animals adaptokhit prevented decline in the proliferative activity of lymphocytes further into adaptation. Also, at the final stage of observation following canceling the preparation there were signs of normalization of the allergologic status of primates in the experimental group. Therefore, adaptokhit can be used as an alimentary supplement to strengthen the colony resistance of animals in extreme conditions.     

Clinical and laboratory characteristics of cryptosporidiosis in Turkmenistan

AIM: The study of clinical symptoms of gastrointestinal lesions in subjects invaded with cryptosporidia. MATERIALS AND METHODS: From 1994 to 1997 383 patients with monocryptosporidiasis were observed. 75.7% of them were children. Cryptosporidia oocysts were identified in fecalia using Fulleborn technique. The specimens were stained according to Cill-Nilsson. RESULTS: Clinically, the invasion was characterized by acute onset, severe course in children, involvement of the whole gastrointestinal and respiratory tracts. CONCLUSION: Monocryptosporidiasis runs in Turkmenistan a more severe course compared to countries with moderately hot climate.