Molecular mobility of polymeric implants and acute inflammatory response: An experimental study in mice

An overlooked factor in biomaterial research is the surface molecular flexibility for polymer based implants. The mobility of the polymer chains provides a way for the surface to adapt itself to the environment. This is relevant when the implant comes in contact with a biological fluid and its constituents. By changing the length of the alkyl side chain of poly(alkyl methacrylates) (PAMAs) an interesting opportunity is provided where it is possible to study the surface molecular mobility without changing the surface hydrophobicity, nor does it introduce any additives or any changes in the degree of polymer cross-linking. Four variants of PAMAs were implanted in the peritoneum of Balb/c mice using a well described setup. End points were taken after 18 h and estimations of inflammatory cell recruitment and implant-associated cells were studied. Relationship between surface molecular mobility and inflammatory cell recruitment as well as surface-associated cells was noted.     

Dual-trap technique for reduction of low-frequency noise in force measuring optical tweezers

High-resolution long-time force measurements by optical tweezers are often limited by low-frequency (1/f) noise. A dual-trap technique is presented that can reduce such noise in the force signal. It incorporates a second trap (a reference trap) that probes the noise in the system and it is based upon the assumption that the low-frequency parts of the noise from the two traps are correlated. A subtraction of the low-frequency signal from the reference trap from the signal from the force measuring trap will therefore yield a net signal that is significantly less influenced by noise. It is shown that this dual-trap technique can reduce the noise in the force signal up to 60% depending on detection bandwidth.     

2.24 Structural and electrical properties of discontinuous gold films on glass

In the present work thin gold films are investigated in the thickness interval 10–160 Å. The films are deposited in UHV on to glass substrates at room temperature and with an electric field in the substrate plane. In the thickness interval 10–45 Å the film is discontinuous and the size distribution of the islands as determined from electron micrographs is described with a log-normal distribution function. The onset of the in situ electrical conduction at the average thickness 45 Å makes it possible to measure the film resistance as a function of the film thickness during the condensation. At the thickness 53 Å stable metallic continuous paths are formed and the film achieves metallic properties. The fraction of the surface covered with material and the island density are measured 20 h after the deposition. Both these entities change rapidly at the stage when the film grows to be metallic continuous.     

3.4 Influence of condensation surface temperature on macrostructure in evaporated chromium films

Chromium, with a Vickers diamond hardness of up to 1200 has been vapor deposited on die cast zinc substrates. In each coating cycle up to six specimens with equal initial condition but different thicknesses could be made. A macrostructure of cracks was found in the films. It is very like the cracking network obtained in electrodeposited chromium. The cracks are caused by intrinsic stress in the films. The ‘mean distance between cracks’ was measured as a function of film thickness, deposition rate and bulk substrate temperature.The hardness is given as a function of ‘mean distance between cracks’. The condensation surface is defined and its temperature dependence on deposition parameters is discussed. Using the concept of condensation surface temperature and the linear expansion coefficient of the film material an atomic model is given to explain the ‘mean distance between cracks’ dependence on the deposition parameters.     

Plastic deformation at a stably growing crack tip

The deformations around the tip of a straight, through-the-thickness crack in sheets of cellulose nitrate, celluloid, in tension have been studied, as well before as after the onset of stable crack growth. A tensile testing apparatus and a device for engraving a fine grid directly on the specimen sheet have been designed. The development of the plastic enclave also after the start of stable crack extension has been studied in connexion with investigations of the deformations in gross fields of size up to 30 × 40 mm² performed on a digital computer (line element length 1 or 0.5 mm, virgin crack length 80 mm). Unexpected deformations very close to the crack tip were found in the near field where line elements of length 50 µm were analyzed. A modified Dugdale model where the cohesive stresses vary linearly is examined and is applied on polyvinylehloride, PVC, specimen the thickness of which is strongly reduced near the crack tip. A method for measurement of thickness changes of the order of a few µm by tracing isohypses is described. Finally, to enable comparison with the experimental results, the elastic deformation pattern for a square mesh in the plane strain and plane stress case is obtained by a digital computer.

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Zusammenfassung Es wurden die Verformungen an der Spitze eines geraden, über die gesamte Dicke durchgehenden Risses in Spannungen unterworfenen Tafeln aus Zellulosenitrat (Zelluloïd) untersucht. Dies geschah sowohl vor als auch nach der Einstellung einer gleichmäßigen Rißfortpflanzungsgeschwindigkeit. Hierzu wurde eine Zugversuchsapparatur sowie ein Verfahren zur Gravierung eines Rasters, direkt auf die Probe, entwickelt.Die Ausbildung der plastischen Enklave, auch nach Einstellung einer gleichmäßigen Rißausweitung, wurde in Verbindung mit Studien der Verformung in Grobrastern his 30 × 40 mm mittels Digitalrechnern, untersucht (Länge einer Elementarlinie l oder 0,5 mm; Originallänge des Risses 80 mm). Direkt an der Rißspitze, wo Elementarlinien mit einer Länge von 50 µm untersucht wurden, konme man unerwartete Verformungen feststellen. Es wurde ein abgewandeltes Dugdale-Modell bei dem die Kohäsionsspannungen sich linear verändern untersucht and auf Polyvinylchlorid PVC-Proben, deren Dicke sich an der Rißspitze stark reduziert, angewandt. Es wird ein Verfahren zur Messung von Änderungen der Dicke um wenige µm beschrieben. Um einen Vergleich mit den experimentellen Ergebnissen zu ermöglichen, wird zum Schluß mit Hilfe eines Digitalrechners das elastische Verformungsmodell für Quadratraster, im Falle planer Verformung and eines planen Spannungszustandes berechnet.

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Résumé On a étudié les déformations qui se produisent au voisinage de l'extrémité d'une fissure droite et traversante dans des feuilles de nitrate de cellulose (celluloid) soumises à traction, et ce avant et après établissement d'un régime stable de propagation. A cet effet, on a mis au point un équipement d'essai de traction et une technique permettant de tracer une grille directement sur l'éprouvette. Le développement d'une enclave plastique dès que l'éxtension de la fissure devient stable a été etudie en recherchant les déformations survenant dans des réseaux grossiers, allant jusqu'à 30 × 40 mm², a l'áide d'un calculateur digital (longueur d'une ligne élémentaire: 1 ou 0,5 mm, longueur de fissure initiale: 80 mm). Dans un réseau plus fin, où les lignes élémentaires avaient une longueur de 50 microns, on a analysé, au voisinage immédiat de l'éxtrémité de la fissure, des déformations inattendues.On a examiné l'ápplication d'un modèle du Dugdale modifié, où les contraintes de cohésion varient linéairement, à des éprouvettes de PVC, dont l'épaisseur subit des réductions considérables près de l'extrémité de la fissure. On décrit une méthode de mesure des variations d'épaisseur de l'órdre de quelques microns, qui est basée sur le tracé de courbes de niveaux.Enfin, pour permettre une comparaison avec les résultats expérimentaux, on a établi par un calculateur digital l'állure de la déformation élastique dans un réseau carré pour les cas d'état plan de déformation et d'état plan de tension.

     

1.4 Fast volume determination using a differential capacitance manometer

The reliable pressure readings with a capacitance manometer are the basis for a new type of instrument designed for accurate and rapid measurements of capillary volumes. The basic measuring principle is based on expansion of air atmospheric pressure enclosed in a known volume into a pre-evacuated cylinder so that the enclosed volume will cause a reduced pressure in the cyllinder. The same is done with the air in an unknown volume into another identical pre-evacuated cylinder. A differential capacitance manometer is then used to measure the difference in pressure between the two cylinders with a high degree of accuracy. This difference in pressure is shown to be ainear function of the difference in volume between the unknown and the known volume. The accuracy and repeatability of the instrument is better than 0.5%.