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291.
S Clemens DM Antosiewicz JM Ward DP Schachtman JI Schroeder 《Canadian Metallurgical Quarterly》1998,95(20):12043-12048
Nonessential metal ions such as cadmium are most likely transported across plant membranes via transporters for essential cations. To identify possible pathways for Cd2+ transport we tested putative plant cation transporters for Cd2+ uptake activity by expressing cDNAs in Saccharomyces cerevisiae and found that expression of one clone, LCT1, renders the growth of yeast more sensitive to cadmium. Ion flux assays showed that Cd2+ sensitivity is correlated with an increase in Cd2+ uptake. LCT1-dependent Cd2+ uptake is saturable, lies in the high-affinity range (apparent KM for Cd2+ = 33 microM) and is sensitive to block by La3+ and Ca2+. Growth assays demonstrated a sensitivity of LCT1-expressing yeast cells to extracellular millimolar Ca2+ concentrations. LCT1-dependent increase in Ca2+ uptake correlated with the observed phenotype. Furthermore, LCT1 complements a yeast disruption mutant in the MID1 gene, a non-LCT1-homologous yeast gene encoding a membrane Ca2+ influx system required for recovery from the mating response. We conclude that LCT1 mediates the uptake of Ca2+ and Cd2+ in yeast and may therefore represent a first plant cDNA encoding a plant Ca2+ uptake or an organellar Ca2+ transport pathway in plants and may contribute to transport of the toxic metal Cd2+ across plant membranes. 相似文献
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The attentional myopia model of behavioral control was tested in a study of food consumption by chronic dieters. According to the model, when individuals' attentional capacity is limited, their behavior will be disproportionately influenced by highly salient internal and external cues to the exclusion of more distal stimuli. In situations that normally feature conflicting behavioral pressures, such a state of narrowed attention is likely to result in abnormally disinhibited behavior when cues promoting action are more salient and to result in abnormally restrained behavior when inhibiting cues are more salient. In addition to supporting the predictions of the model, this study provided the first evidence for enhanced behavioral control under conditions of limited attention. Participants' responses to a thought-reporting measure also provided evidence for the role played by attentional myopia in the control of eating. Implications for a broad array of regulatory successes and failures are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
294.
Ward C. Smith M. Jeffries A. Adams D. Hudson J. 《Electronics & Communication Engineering Journal》1996,8(4):191-200
Cellular communications have become the success story of the late 20th century. In just 15 years the global cellular subscriber base has grown to 60 million and it is forecast to exceed 350 million by early next century. This rapid growth has created the dichotomy for cellular operators of increasing pressure from customers to improve coverage, capacity and quality within their networks whilst the ever growing competition in the cellular marketplace demands that operational costs be reduced to the lowest possible level. One development which can assist network operators in reconciling these conflicting demands is the smart antenna. However, the successful deployment of smart antennas will require a much greater understanding of the characteristics of signals within the mobile/base-station transmission path. The measurement equipment described in this paper has enabled the acquisition of data which contributes significantly towards the understanding of these propagation path characteristics 相似文献
295.
D Ornadel JA Ledermann K Eagle RB Pedley G Boxer SE Ward Y Olabiran J Bomanji 《Canadian Metallurgical Quarterly》1998,77(1):103-109
In this study, we examined the acute anticonvulsant spectrum of (1) dizocilpine (0.03-3 mg/kg), CGS 19755 (1-10 mg/kg), and 7-chlorokynurenic acid (1-100 nmol) (NMDA receptor/ionophore complex antagonists); (2) muscimol (0.1-10 nmol; direct GABA(A) agonist); (3) YM90K (3-10 mg/kg; AMPA receptor antagonist); and (4) diazepam (2 and 5 mg/kg) and carbamazepine (5 and 20 mg/kg), two standard anticonvulsants, using the partially-kindled hippocampal model for epileptic seizures in freely moving rats. The anticonvulsant effect of these compounds were assessed by determining (1) the afterdischarge (AD), which is indicative of the severity of the seizure and related to seizure maintenance, and (2) the pulse number threshold (PNT), which is indicative of the seizure threshold or initiation. In addition, ataxia, a measure of CNS dysfunction, was assessed for each compound. Overall, our results indicated that the anticonvulsant compounds examined could be classified into three categories based on effects on the AD and PNT: (1) elevation of PNT (carbamazepine, dizocilpine, CGS 19755 and 7-chlorokynurenic acid); (2) reduction of AD (diazepam and muscimol); and (3) mixed action, i.e., increased PNT and decreased AD (YM90K). Behavioral data indicated that all compounds, except carbamazepine, produced a dose- or concentration-dependent ataxia. Overall, our results suggest that NMDA receptors play a role in seizure initiation, whereas the GABA(A) receptors appear to be involved in seizure maintenance and AMPA receptors may be involved in both phenomena. 相似文献
296.
Finite element ring rolling simulation by conventional Lagrangian codes carries an excessive computational cost. The main reason for this is the large number of incremental stages typically required to complete a full simulation. The nature of ring rolling however means that the amount of deformation taking place in a given increment is relatively small compared with typical metal forming processes. This paper describes measures that make the analysis of ring rolling a practicable proposition. The resulting model is based on a threefold approach, comprising the finite element flow formulation, an arbitrary Lagrangian Eulerian update strategy, and a novel iterative solution scheme called the successive preconditioned conjugate gradient method. The approach exploits the slowly evolving nature of the problem with the effect of reducing the time penalty for each deformation increment. In addition, a number of issues specific to ring rolling have been addressed including the problem of how the mandrel interface is dealt with for arbitrarily shaped rollers. The importance of addressing this particular issue is also illustrated. The method is validated by comparison with earlier experimental work and previously developed models for both pure radial, and radial–axial ring rolling. 相似文献
297.
RA Malik S Tesfaye SD Thompson A Veves A Hunter AK Sharma JD Ward AJ Boulton 《Canadian Metallurgical Quarterly》1994,48(2):236-245
Morphometric techniques were employed to assess perineurial capillary abnormalities in the sural nerve of 20 diabetic patients with neuropathy and 10 normal control subjects. Structural abnormalities were related to quantitative neurophysiological and neuropathological measures of neuropathy. Perineurial capillary endothelial cell area (P < 0.001) and endothelial cell profile number (P < 0.01) were increased and luminal area (P < 0.001) was reduced in diabetic patients when compared with control subjects. A significant relationship was observed between endothelial cell hyperplasia and measures of neuropathic severity. These findings provide evidence for perineurial capillary luminal occlusion due primarily to both endothelial cell hypertrophy and hyperplasia. Such a reduction in luminal size is expected to reduce transperineurial and hence endoneurial blood flow, resulting in endoneurial hypoxia and hence human diabetic neuropathy. 相似文献
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Myxococcus xanthus is a gram-negative bacterium which has a complex life cycle. Autochemotaxis, a process whereby cells release a self-generated signaling molecule, may be the principal mechanism facilitating directed motility in both the vegetative swarming and developmental aggregation stages of this life cycle. The process requires the Frz signal transduction system, including FrzZ, a protein which is composed of two domains, both showing homology to the enteric chemotaxis response regulator CheY. The first domain of FrzZ (FrzZ1), when expressed as bait in the yeast two-hybrid system and screened against a library, was shown to potentially interact with the C-terminal portion of a protein encoding an ATP-binding cassette (AbcA). The activation domain-AbcA fusion protein did not interact with the second domain of FrzZ (FrzZ2) or with two other M. xanthus response regulator-containing proteins presented as bait, suggesting that the FrzZ1-AbcA interaction may be specific. Cloning and sequencing of the upstream region of the abcA gene showed the ATP-binding cassette to be linked to a large hydrophobic, potentially membrane-spanning domain. This domain organization is characteristic of a subgroup of ABC transporters which perform export functions. Cloning and sequencing downstream of abcA indicated that the ABC transporter is at the start of an operon containing three open reading frames. An insertion mutation in the abcA gene resulted in cells displaying the frizzy aggregation phenotype, providing additional evidence that FrzZ and AbcA may be part of the same signal transduction pathway. Cells with mutations in genes downstream of abcA showed no developmental defects. Analysis of the proposed exporter role of AbcA in cell mixing experiments showed that the ABC transporter mutant could be rescued by extracellular complementation. We speculate that the AbcA protein may be involved in the export of a molecule required for the autochemotactic process. 相似文献