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71.
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PB DePetrillo 《Canadian Metallurgical Quarterly》1997,68(5):1863-1869
Calcium-activated neutral protease activity was determined in PC12 cells exposed to ethanol for 96 h using a fluorescence-based assay with N-succinyl-Leu-Tyr 7-amido-4-methylcoumarin as the substrate. Stimulated activity was measured at high (1,400 microM) or low (140 microM) Ca2+ concentrations in the presence of 20 microM ionomycin. Kinetic parameters were derived by fitting a model relating fluorescence intensity to time: F(t) = F(final)*(1 - e(-k(obs)t). Cell extracts were subjected to nondenaturing gel electrophoresis and casein zymography with quantification of the activity of the two calpain isoforms. Exposure to ethanol significantly decreased whole cell calpain activity measured by k(obs) beginning at 20 mM, to 27.8% of control at 1,400 microM Ca2+ and 29.2% of control at 140 microM Ca2+ in the presence of 20 microM ionomycin. No changes in mu-calpain or m-calpain activities were found in cell extracts from cells exposed to 20 mM ethanol, whereas at 40 and 80 mM ethanol, significant decreases in both mu-calpain and m-calpain activities were discovered. 相似文献
73.
Aortic valve endocarditis commonly leads to the formation of a root abscess, but fistulae are uncommon. The echocardiographic findings in a patient with Streptococcus viridans endocarditis of a prosthetic aortic valve associated with a fistula between the aorta and the left atrium are presented. The diagnosis was made by transthoracic echocardiography, although the transesophageal study gave higher resolution views and allowed a more confident exclusion of mitral valve involvement. 相似文献
74.
GJ Harris EH Aylward CE Peyser GD Pearlson J Brandt JV Roberts-Twillie PE Barta SE Folstein 《Canadian Metallurgical Quarterly》1996,53(4):316-324
During oogenesis in Drosophila, germ cells appear in sequential clusters of 16 interconnected cells. The events surrounding the differentiation of these cells are not fully understood. Here we present genetic and morphological analysis of mutations in the gene stand still (stil). Through complementation analyses we have refined the location of this gene to cyological region 49B-C. Our analyses of ovaries from ethylmethane sulfonate (EMS)-induced mutant alleles of this gene suggest that mutations in the stil gene produce a wide range of phenotypic abnormalities, from the absence of germ cells in the most severe alleles, to egg chambers with cytoskeletal defects in the less severe alleles. Our results suggest a role for this gene in specifying or maintaining a cytoskeletal component, with consequences during oogenesis and possibly during germ line sex determination. 相似文献
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Ha W. Gambin V. Bank S. Wistey M. Yuen H. Seongsin Kim Harris J.S. Jr. 《Quantum Electronics, IEEE Journal of》2002,38(9):1260-1267
The boom in fiber-optic communications has caused a high demand for GaAs-based lasers in the 1.3-1.6-μm range. This has led to the introduction of small amounts of nitrogen into InGaAs to reduce the bandgap sufficiently, resulting in a new material that is lattice matched to GaAs. More recently, the addition of Sb has allowed further reduction of the bandgap, leading to the first demonstration of 1.5-μm GaAs-based lasers by the authors. Additional work has focused on the use of GaAs, GaNAs, and now GaNAsSb barriers as cladding for GaInNAsSb quantum wells. We present the results of photoluminescence, as well as in-plane lasers studies, made with these combinations of materials. With GaNAs or GaNAsSb barriers, the blue shift due to post-growth annealing is suppressed, and longer wavelength laser emission is achieved. Long wavelength luminescence out to 1.6 μm from GaInNAsSb quantum wells, with GaNAsSb barriers, was observed. In-plane lasers from these samples yielded lasers operating out to 1.49 μm, a minimum threshold current density of 500 A/cm2 per quantum well, a maximum differential quantum efficiency of 75%, and pulsed power up to 350 mW at room temperature 相似文献
77.
78.
When the lines go down [telecommunications] 总被引:1,自引:0,他引:1
Telecommunications planning for national security and emergency preparedness in the post-divestiture (of AT&T) environment is examined. To ensure emergency telecommunications, a web of emergency structures, plans, and procedures are in place, with links from the local provider level to the national level. Involved in this web are: individual companies and providers, regional and national corporate entities, and state and local emergency management centers. Some of the measures in place are discussed. The augmentations or other equipment that can be deployed in any emergency and the role of satellite and wireless communication are described. The importance of human preparation is addressed. Lessons learned in past emergencies and the potential impact of emerging technologies in future emergencies are discussed 相似文献
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