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61.
VL Beggs SB Willis EL Maislen TM Stokes D White M Sanford A Becker S Barber PC Pawlow C Downs 《Canadian Metallurgical Quarterly》1998,12(4):72-86
Short hospitalizations for patients undergoing coronary artery bypass grafting (CABG) require continuous nursing evaluation of patients' discharge education. Six institutions collaborated in surveying 300 postoperative patients with CABG to identify learning priorities and patients' perceptions of the effectiveness of discharge education. Data analysis from the self-administered questionnaire demonstrated consistent patient priorities across institutions. Differences in teaching methods and content did not affect perceived preparedness or importance scores. Regional experience demonstrates that variable teaching efforts meet patients' priorities and provide high overall patient preparedness for discharge. Patients with the shortest hospitalizations had higher preparedness scores. 相似文献
62.
Both estradiol and nonylphenol (NP) inhibited hepatic microsomal 7-ethoxyresorufin O-deethylase (EROD) activity of beta-naphthoflavone-treated rats. Enzyme kinetic analyses (Lineweaver-Burk plots) using different estradiol and NP concentrations with graded increases in the concentrations of the substrate, ethoxyresorufin, showed that the inhibition was of a competitive nature at all concentrations of estradiol or NP used. Thus, the mechanism by which NP inhibits EROD activity is similar to that of estradiol. NP, however, was much less potent than estradiol. Young rats treated in vivo with 80 mg/kg body weight of NP demonstrated a slight but significant decrease in their hepatic microsomal EROD activity and CYP1A protein as measured by western blot analysis. In addition, treatment with NP led to a decrease in the steady-state levels of hepatic CYP1A mRNA in rats, suggesting that NP acted at the pre-translational level. The competitive nature of inhibition by NP on hepatic microsomal EROD activity indirectly suggests that this compound is a possible substrate of the CYP1A enzyme. Furthermore, NP had a moderate modulating effect on the expression of CYP1A in rat liver. 相似文献
63.
JC Meltzer V Sanders PC Grimm N Chiasson HJ Hoeltke KL Garrett AH Greenberg DM Nance 《Canadian Metallurgical Quarterly》1998,19(8-9):1351-1355
The application of nonradioactive RNA probes for Northern blotting offers the advantage of a rapid turn-around time for results without the loss of sensitivity for target mRNA detection. However, a problem that has impeded the widespread use of nonradioactive RNA probes for use in Northern blotting is the difficulty in stripping these probes from nylon membranes after hybridization. In this report we describe two protocols for stripping digoxigenin (Dig)-labeled RNA probes from nylon membranes. One protocol utilizes a phosphate-buffered formamide stripping solution to remove nonchemically modified (regular) RNA probes while the other method utilizes strippable probes that were produced with a chemically modified nucleotide (CTP) and removed by a specific stripping solution. This latter method was developed by Ambion Inc. and is called Strip-EZ. We also describe a protocol for the detection of two separate rat mRNAs using both biotin and digoxigenin-labeled RNA probes that does not require stripping the membrane after hybridization. Finally, we describe the use of another new labeling technology, called Chem-Link, that quickly and conveniently labels RNA for use in Northern blotting. 相似文献
64.
65.
Fernando Ruiz 《微电脑世界》2012,(11):128-130
随着Android终端设备的快速普及,这个移动互联的大时代,不仅促成了智能设备的如日中天,同时也为罪恶之手打开了一扇全新的大门。Android.FakeInstaller就是这样一个广泛传播的移动恶意软件系列。它曾假冒奥运会赛事成绩应用、Skype、Flash Player、Opera和许多其他流行应用程序。事实上,在移动恶意软件界,FakeInstaller系列是我 相似文献
66.
A method of compensating for the lag of the video cameras typically used in angiographic systems is presented for use in sequences of digitized X-ray images. The lag effect is reduced by a straightforward weighted subtraction, which has the undesirable side effect of increasing noise. By superimposing several lag-corrected and appropriately shifted images, however, the signal-to-noise ratio can be restored. The algorithm uses the phase-correlation method to measure the two-dimensional shift of a mobile coronary arterial structure. Processing is confined to a rectangular area of interest (AOI), which encloses a feature of clinical significance. The differences of the phases of the Fourier transforms of two frames is computed, combined with an appropriate filter, and inverse Fourier-transformed to produce a phase-correlation image. The vector separation from the origin of image space of the peak of the phase-correlation image is the estimate of the shift of the artery's position in the second frame as compared to the first. The isolation of the AOI from the surrounding image is achieved by the application of a window and correction for any linear trend in the background intensity. 相似文献
67.
"This paper is a report on continuing research on the application of age profiles in migration analysis. In particular, it is an update of previous work published in this journal with an analysis of 1981 Census data for local authority areas in England. The changes in migration patterns over the intercensal period from 1971 to 1981 are described." The focus is on internal migration 相似文献
68.
A. V. Sverdlin G. E. Totten C. Bates L. M. Jarvis 《Metal Science and Heat Treatment》1996,38(6):248-251
The development of methods for evaluating and predicting the variation of the properties of polymer quenching media in operation requires an analysis of the quenching factor, which characterizes the degree of decomposition of the solid solution in quenching, which decreases the strength (hardness) of the alloy by a given value. In the present paper the quenching factorQ is used to compare the properties of aluminum alloys quenched in hot water and aqueous solutions of polymers.Translated from Metallovedenie i Termicheskaya Obrabotka Metallov, No. 6, pp. 14 – 17, June, 1996. 相似文献
69.
70.
Bates Paul A.; McGregor Malcolm J.; Islam Suhail A.; Sattentau Quentin J.; Sternberg Michael J.E. 《Protein engineering, design & selection : PEDS》1989,3(1):13-21
A predicted three-dimensional structure of the two N-terminalextracellular domains of human CD4 antigen, a cell surface glycoprotein,is reported. This region of CD4, particularly the first domain,has been identified as containing the binding region for theenvelope gp120 protein of the human immuno-deficiency virus.The model was predicted based on the sequence homology of eachdomain with the variable light chain of immunoglobulins. Theframework ß-sheet regions were taken from the crystalcoordinates of REI. For one region in the first domain of CD4there was an ambiguity in the alignment with REI and two alternatemodels are presented. Loops connecting the framework were modeledfrom fragments selected from a database of main chain coordinatesfrom all known protein structures. Residues identified as involvedin binding gp120 have been located in several other studieswithin the first domain of CD4. Epitopes from eight monoclonalantibodies have been mapped onto residues in both domains. Competitionof these antibodies with each other and with gp120 can be interpretedfrom the structural model. 相似文献