全文获取类型
收费全文 | 1823篇 |
免费 | 1篇 |
专业分类
化学工业 | 14篇 |
机械仪表 | 1篇 |
建筑科学 | 7篇 |
能源动力 | 1篇 |
轻工业 | 7篇 |
石油天然气 | 17篇 |
无线电 | 1篇 |
一般工业技术 | 2篇 |
冶金工业 | 1747篇 |
自动化技术 | 27篇 |
出版年
2022年 | 1篇 |
2021年 | 1篇 |
2020年 | 1篇 |
2019年 | 1篇 |
2018年 | 1篇 |
2017年 | 1篇 |
2016年 | 1篇 |
2015年 | 1篇 |
2014年 | 7篇 |
2013年 | 4篇 |
2012年 | 3篇 |
2011年 | 1篇 |
2010年 | 5篇 |
2009年 | 5篇 |
2008年 | 1篇 |
2007年 | 3篇 |
2006年 | 2篇 |
2005年 | 5篇 |
2004年 | 2篇 |
2003年 | 17篇 |
2002年 | 6篇 |
2001年 | 4篇 |
2000年 | 3篇 |
1999年 | 54篇 |
1998年 | 522篇 |
1997年 | 292篇 |
1996年 | 228篇 |
1995年 | 110篇 |
1994年 | 98篇 |
1993年 | 104篇 |
1992年 | 11篇 |
1991年 | 30篇 |
1990年 | 32篇 |
1989年 | 23篇 |
1988年 | 37篇 |
1987年 | 22篇 |
1986年 | 22篇 |
1985年 | 13篇 |
1983年 | 4篇 |
1982年 | 10篇 |
1981年 | 12篇 |
1980年 | 11篇 |
1978年 | 4篇 |
1977年 | 36篇 |
1976年 | 71篇 |
1975年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有1824条查询结果,搜索用时 15 毫秒
41.
We have isolated an insertional mutant of Dictyostelium discoideum that aggregated rapidly and formed spores and stalk cells within 14 h of development instead of the normal 24 h. We have shown by parasexual genetics that the insertion is in the rdeA locus and have cloned the gene. It encodes a predicted 28 kDa protein (RdeA) that is enriched in charged residues and is very hydrophilic. Constructs with the DNA for the c-Myc epitope or for the green fluorescent protein indicate that RdeA is not compartmentalized. RdeA displays homology around a histidine residue at amino acid 65 with members of the H2 module family of phosphotransferases that participate in multistep phosphoryl relays. Replacement of this histidine rendered the protein inactive. The mutant is complemented by transformation with the Ypd1 gene of Saccharomyces cerevisiae, itself an H2 module protein. We propose that RdeA is part of a multistep phosphorelay system that modulates the rate of development. 相似文献
42.
VL Beggs SB Willis EL Maislen TM Stokes D White M Sanford A Becker S Barber PC Pawlow C Downs 《Canadian Metallurgical Quarterly》1998,12(4):72-86
Short hospitalizations for patients undergoing coronary artery bypass grafting (CABG) require continuous nursing evaluation of patients' discharge education. Six institutions collaborated in surveying 300 postoperative patients with CABG to identify learning priorities and patients' perceptions of the effectiveness of discharge education. Data analysis from the self-administered questionnaire demonstrated consistent patient priorities across institutions. Differences in teaching methods and content did not affect perceived preparedness or importance scores. Regional experience demonstrates that variable teaching efforts meet patients' priorities and provide high overall patient preparedness for discharge. Patients with the shortest hospitalizations had higher preparedness scores. 相似文献
43.
Both estradiol and nonylphenol (NP) inhibited hepatic microsomal 7-ethoxyresorufin O-deethylase (EROD) activity of beta-naphthoflavone-treated rats. Enzyme kinetic analyses (Lineweaver-Burk plots) using different estradiol and NP concentrations with graded increases in the concentrations of the substrate, ethoxyresorufin, showed that the inhibition was of a competitive nature at all concentrations of estradiol or NP used. Thus, the mechanism by which NP inhibits EROD activity is similar to that of estradiol. NP, however, was much less potent than estradiol. Young rats treated in vivo with 80 mg/kg body weight of NP demonstrated a slight but significant decrease in their hepatic microsomal EROD activity and CYP1A protein as measured by western blot analysis. In addition, treatment with NP led to a decrease in the steady-state levels of hepatic CYP1A mRNA in rats, suggesting that NP acted at the pre-translational level. The competitive nature of inhibition by NP on hepatic microsomal EROD activity indirectly suggests that this compound is a possible substrate of the CYP1A enzyme. Furthermore, NP had a moderate modulating effect on the expression of CYP1A in rat liver. 相似文献
44.
JC Meltzer V Sanders PC Grimm N Chiasson HJ Hoeltke KL Garrett AH Greenberg DM Nance 《Canadian Metallurgical Quarterly》1998,19(8-9):1351-1355
The application of nonradioactive RNA probes for Northern blotting offers the advantage of a rapid turn-around time for results without the loss of sensitivity for target mRNA detection. However, a problem that has impeded the widespread use of nonradioactive RNA probes for use in Northern blotting is the difficulty in stripping these probes from nylon membranes after hybridization. In this report we describe two protocols for stripping digoxigenin (Dig)-labeled RNA probes from nylon membranes. One protocol utilizes a phosphate-buffered formamide stripping solution to remove nonchemically modified (regular) RNA probes while the other method utilizes strippable probes that were produced with a chemically modified nucleotide (CTP) and removed by a specific stripping solution. This latter method was developed by Ambion Inc. and is called Strip-EZ. We also describe a protocol for the detection of two separate rat mRNAs using both biotin and digoxigenin-labeled RNA probes that does not require stripping the membrane after hybridization. Finally, we describe the use of another new labeling technology, called Chem-Link, that quickly and conveniently labels RNA for use in Northern blotting. 相似文献
45.
46.
Fernando Ruiz 《微电脑世界》2012,(11):128-130
随着Android终端设备的快速普及,这个移动互联的大时代,不仅促成了智能设备的如日中天,同时也为罪恶之手打开了一扇全新的大门。Android.FakeInstaller就是这样一个广泛传播的移动恶意软件系列。它曾假冒奥运会赛事成绩应用、Skype、Flash Player、Opera和许多其他流行应用程序。事实上,在移动恶意软件界,FakeInstaller系列是我 相似文献
47.
48.
49.
CM Otto MC Mickel JW Kennedy EL Alderman TM Bashore PC Block JA Brinker D Diver J Ferguson DR Holmes 《Canadian Metallurgical Quarterly》1994,89(2):642-650
BACKGROUND: To identify predictors of long-term outcome after balloon aortic valvuloplasty, we analyzed data on 674 adults (mean age, 78 +/- 9 years; 56% were women) undergoing this procedure at 24 clinical centers who had a mean initial increase in aortic valve area of 0.3 cm2. METHODS AND RESULTS: Baseline data included clinical, echocardiographic, and catheterization variables. Follow-up data included mortality, cause of death, rehospitalization, 6-month echocardiography, and functional status. Kaplan-Meier curves and log-rank tests were used to evaluate survival in subgroups. Multivariate Cox regression models were used to identify independent predictors of survival. Overall survival was 55% at 1 year, 35% at 2 years, and 23% at 3 years, with the majority of deaths (70%) classified as cardiac by an independent review committee. Rehospitalization was common (64%), although 61% of survivors at 2 years reported improved symptoms. Echocardiography at 6 months (n = 115) showed restenosis from the postprocedural valve area of 0.78 +/- 0.31 cm2 to 0.65 +/- 0.25 cm2 (P < .0001). With stepwise multivariate analysis, sequentially adding clinical, echocardiographic, and catheterization variables, the overall model identified independent predictors of survival as baseline functional status, baseline cardiac output, renal function, cachexia, female gender, left ventricular systolic function, and mitral regurgitation. Baseline and postprocedural variables were examined to identify which subgroup of patients has the best outcome after aortic valvuloplasty. A "lower-risk" subgroup (28% of the study population), defined by normal left ventricular systolic function and mild clinical functional limitation, had a 3-year survival of 36% compared with 17% in the remainder of the study group. CONCLUSIONS: Long-term survival after balloon aortic valvuloplasty is poor with 1- and 3-year survival rates of 55% and 23%, respectively. Although survivors report fewer symptoms, early restenosis and recurrent hospitalization are common. 相似文献
50.