Compressive strength of coated rigid-rod polymer fibres

One limitation to the use of high-strength/high-modulus rigid-rod polymer fibres like poly-(p-phenylene benzobisthiazole) (PBZT) and poly-(p-phenylene benzobisoxazole) (PBZO) in composite structures is their low compressive strength. Various theories have been developed to predict compressive strength of rigid-rod fibres. In this study the critical buckling stress for rigid-rod fibres with stiff external coatings has been theoretically modelled assuming that the failure mode in compression is the microbuckling of the fibrils in shear. Our model predicts that significant improvement in fibre compressive strength will occur only when relatively thick coatings, with thickness to diameter (t/D) ratios in excess of > 0.05, are used. Experimentally measured compressive strength of aluminium coated PBZT fibres shows values in good agreement to the theory at t/D ratios of 0.006 and below. Factors related to the selection of suitable coating materials and problems associated with establishing coating performance are identified.Nomenclature P axial compressive load - P _f axial compressive load on the fibre - P _c axial compressive load on the coating - P _cr ⁱ critical buckling load in the ith case - _cr critical buckling stress - _co compressive strength of the uncoated fibre - _c compressive strength of the coated fibre - v(x) lateral deflection of a buckled fibril or coating - V _m amplitude of the lateral deflection in the mth mode - m number of half-sine waves in the deflection mode - x coordinate distance along axial direction - y coordinate distance along radial direction - coordinate distance along circumferential direction - l length of the buckling unit - N number of fibrils in the fibre - D fibre diameter - d fibril diameter - t coating thickness - I _f moment of inertia of the fibril - A _f cross-sectional area of the fibril - E _f tensile modulus of the fibre - E _c tensile modulus of the coating material - E tensile modulus of the coated fibre - G torsional shear modulus of the fibre - v_c Poisson's ratio of the coating material - _f density of the fibre - _c density of the coating material - density of the coated fibre - U _f strain-energy change in the fibre - U _c strain-energy change in the coating - T _f external work done on the fibre - T _c external work done on the coating - d/D - t/D     

Energy metabolism and fuel mobilization: from the perioperative period to recovery

PURPOSE: Previous studies of monochromatic visual evoked potentials confirm the strong suppression of (the cortical representation of) paracentral retinal areas of functionally amblyopic eyes, by a flat response to a blue stimulus. A clinical trial stimulating these areas with blue light was encouraging, and justified a prospective comparison of this treatment with conventional classic treatment. SUBJECTS AND METHODS: In 50 strabismic children with amblyopia, the blue filter treatment protocol (flash stimulation with, and the wearing of, a blue filter during occlusion of the better eye for one hour daily) was compared with the classical treatment (full time total occlusion by patch) in a prospective matched and randomized study. Patients 3 to 7 years old without previous treatment and a visual acuity up to 0.3 were admitted to the study. Visual acuity and fixation behavior were used as the parameters of comparison. Visual Evoked Potentials by monochromatic flashes were also studied. Results of treatment were compared after 6 months. RESULTS: The visual acuity outcome for the blue filter treatment was "statistically significantly" better (p=0.005). The greatest improvement was seen in the subgroup of children with eccentric fixation (p=0.01). Fixation behavior also showed a better outcome from the blue filter treatment (p=0.05) favoring especially children between 3 and 5 years. In children of this age with a visual acuity better than 0.1 we found a very "statistically significant" difference between the two treatments (p=0.004). In children 3 to 5 years old with poorer visual acuity we also found a "statistically significant" difference in the two treatments (p=0.04). The interocular difference of amplitude on the Visual Evoked Potentials also demonstrated more improvement in children treated with the blue filter. This treatment improved especially the cortical response to blue flash stimulation, correlating to paracentral retinal areas. CONCLUSIONS: The blue filter treatment protocol provided better results for treating amblyopia than the conventional classic occlusion treatment method. We propose that stimulation of these paracentral retinal areas triggers a better disinhibition of a functionally amblyopic eye.     

Estrogen-induced alteration of mu-opioid receptor immunoreactivity in the medial preoptic nucleus and medial amygdala

The mu-opioid receptor (mu-OR), like most G-protein-coupled receptors, is rapidly internalized after agonist binding. Although opioid peptides induce internalization in vivo, there are no studies that demonstrate mu-OR internalization in response to natural stimuli. In this study, we used laser-scanning microscopy to demonstrate that estrogen treatment induces the translocation of mu-OR immunoreactivity (mu-ORi) from the membrane to an internal location in steroid-sensitive cell groups of the limbic system and hypothalamus. Estrogen-induced internalization was prevented by the opioid antagonist naltrexone, suggesting that translocation was largely dependent on release of endogenous agonists. Estrogen treatment also altered the pattern of mu-ORi at the bright-field light microscopic level. In the absence of stimulation, the majority of immunoreactivity is diffuse, with few definable mu-OR+ cell bodies or processes. After stimulation, the density of distinct processes filled with mu-ORi was significantly increased. We interpreted the increase in the number of mu-OR+ processes as indicating increased levels of internalization. Using this increase in the density of mu-OR+ fibers, we showed that treatment of ovariectomized rats with estradiol benzoate induced a rapid and reversible increase in the number of fibers. Significant internalization was noted within 30 min and lasted for >24 hr after estrogen treatment in the medial preoptic nucleus, the principal part of the bed nucleus, and the posterodorsal medial amygdala. Naltrexone prevented the increase of mu-OR+ processes. These data imply that estrogen treatment stimulates the release of endogenous opioids that activate mu-OR in the limbic system and hypothalamus providing a "neurochemical signature" of steroid activation of these circuits.     

Model selection for covariance structures analysis in nursing research

Covariance structures analysis is often used in nursing research to appraise statistical models reflecting complex human health processes. The model selection approach in covariance structures analysis is designed to select the "best" model from a specified set of theoretically defensible, competing alternatives, all of which are viewed as approximations. Model selection criteria explicitly incorporate both model misfit in the population and sampling error to evaluate the set of models. The result is that interpretability of model parameters and goodness-of-fit are enhanced simultaneously. Relative merits of the model selection approach are identified in light of technical concerns, parsimony, and use of scientific theory in nursing.     

Novel aspects of oxygen diffusion in silicon

Normal diffusion of interstitial oxygen atoms (O_i ) accounts for the rate of oxygen aggregation in silicon for T > 500C. There is evidence for the dissociation of SiO₂ precipitates (Ostwald ripening) and the formation of self-interstitials (I-atoms) to accommodate the local increase in volume. For T < 500 C, measurements of the loss of oxygen atoms from solution indicate that O₂ dimer formation is the rate-limiting process, but dissociation of dimers must be taken into account when modelling this process. Large clusters of up to 10–20 O_i atoms, usually assigned to thermal donor (TD) defects cannot form unless dimer diffusion is much greater (by a factor of 10⁴ to 10⁷ ) than diffusion of O_i atoms and unless there is dissociation of clusters with the emission of dimers. Hydrogen impurities enhance O_i diffusion by a catalytic process and speed up donor formation. Infrared absorption measurements reveal H-O_i complexes and there is also partial passivation of TD defects to produce shallow thermal donors (STDs).     

Immunomicroscopy: resin techniques and on-section labelling with immunocolloidal gold or immunoperoxidase--planning a protocol

On-section immunocytochemistry is divided into two parts: (i) processing of biological tissue for section microscopy and (ii) immunolabelling of sections. Many of the more successful microscopical methods employ delicate aldehyde fixation of biological tissue followed by "sympathetic" processing into an acrylic resin. Processing regimens do not have to be complicated. Simple and cost effective room temperature protocols utilising partial dehydration have been devised and they can be as effective as the more complex low temperature techniques in preserving both ultrastructure and antigenic reactivity. The embedded material can be investigated by either light or electron microscopy. Frozen sections can be cut and immunolabelled but only if the tissue is chemically fixed first, as in resin embedding. Fixation with low concentrations of aldehyde will normally better preserve tissue immunoreactivity but this may be at the expense of good ultrastructure with these protocols. If so, low temperature resin embedding methods or rapid freezing and cryosubstitution can be tried. The choice of processing protocol will determine which acrylic resin to use, as will the preference for subsequent immunolabelling with either colloidal gold or peroxidase/diaminobenzidine (DAB). Both types of labelling system offer advantages to localisation studies and can be used in combination for double or even triple labelling. Silver enhancement of the colloidal gold or DAB allows for improved observation by light microscopy.     

Amperometric biosensor for formic acid in air

The possibility of developing a simple, inexpensive and specific personal passive “real-time” air sampler incorporating a biosensor for formic acid was investigated. The sensor is based on the enzymatic reaction between formic acid and formate dehydrogenase (FDH) with nicotinamide adenine dinucleotide (NAD⁺) as a co-factor and Meldola's blue as mediator. An effective way to immobilise the enzyme, co-factor and Meldola's blue on screen-printed, disposable, electrodes was found to be in a mixture of glycerol and phosphate buffer covered with a gas-permeable membrane. Steady-state current was reached after 4–15 min and the limit of detection was calculated to be below 1 mg/m³. However, the response decreased by 50% after storage at −15°C for 1 day.