Ganglioside content of human platelets--differences in resting and activated platelets

Gangliosides may play functional roles in platelet physiology, therefore this study has been designed to evaluate whether changes in ganglioside composition may occur as a consequence of platelet activation. The results obtained indicate that lactosylceramide and GM3 are the major glycosphingolipids of human platelets. The lipid-bound sialic acid (LBSA) content was 1.27 +/- 0.04 micrograms/mg of protein. Resting platelets did not express GD3; GD3 was synthesized upon platelet activation (24 +/- 8 ng/mg of protein). The stimulation of platelets with adenosine diphosphate showed the appearance of GD3 even in the absence of degranulation. Finally, incorporation of pyrene-labeled GM3 into platelet membranes, followed by stimulation with adenosine diphosphate, resulted in the appearance of a fluorescent band comigrating with GD3. The present studies indicate that sialytransferase activation may occur as an early event following platelet stimulation, leading to GD3 synthesis mainly from the GM3 pool.     

Recognizing arabic handwritten characters using deep learning and genetic algorithms

Automated techniques for Arabic content recognition are at a beginning period contrasted with their partners for the Latin and Chinese contents recognition. There is a bulk of handwritten Arabic archives available in libraries, data centers, historical centers, and workplaces. Digitization of these documents facilitates (1) to preserve and transfer the country’s history electronically, (2) to save the physical storage space, (3) to proper handling of the documents, and (4) to enhance the retrieval of information through the Internet and other mediums. Arabic handwritten character recognition (AHCR) systems face several challenges including the unlimited variations in human handwriting and the leakage of large and public databases. In the current study, the segmentation and recognition phases are addressed. The text segmentation challenges and a set of solutions for each challenge are presented. The convolutional neural network (CNN), deep learning approach, is used in the recognition phase. The usage of CNN leads to significant improvements across different machine learning classification algorithms. It facilitates the automatic feature extraction of images. 14 different native CNN architectures are proposed after a set of try-and-error trials. They are trained and tested on the HMBD database that contains 54,115 of the handwritten Arabic characters. Experiments are performed on the native CNN architectures and the best-reported testing accuracy is 91.96%. A transfer learning (TF) and genetic algorithm (GA) approach named “HMB-AHCR-DLGA” is suggested to optimize the training parameters and hyperparameters in the recognition phase. The pre-trained CNN models (VGG16, VGG19, and MobileNetV2) are used in the later approach. Five optimization experiments are performed and the best combinations are reported. The highest reported testing accuracy is 92.88%.

     

Design Guidelines for the Noise Optimization of a 0.18 μm CMOS Low-Noise Amplifier

This paper presents the design considerations for the noise optimization of fully integrated tuned low-noise amplifiers (LNA) based on the four noise parameters and two-port noise theory. Specifically, this paper provides the design guidelines for a 0.18 μm CMOS tuned LNA. These guidelines give a useful indication to the design tradeoffs associated with noise figure, power dissipation and gate overdrive voltage for the LNA designed using this technology. As a case study, a 10 GHz LNA has been designed using 0.18 μm CMOS technology for a wireless LAN application. The amplifier has a 2.4 dB noise figure with a −13 dBm third-order input intercept point, while drawing 5 mW from a 1.8 V power supply. The results show that the proposed theoretical contours of constant noise figure which relate the gate overdrive voltage and power dissipation can accurately predict the noise performance of a 0.18 μm CMOS LNA design Ahmed A. Youssef received the B.Sc. (Hon.) and M.Sc. degrees both in electrical engineering from Ain Shams University, Cairo, Egypt, in 1998 and 2002, respectively. Since 2003, he has been with the University of Calgary, AB, Canada, where he is currently working toward the Ph.D. degree in RF integrated circuits and systems. Mr. Youssef has joined the Wireless Research Center at TRLab, Alberta, Canada as a research associate in 2004. His research interests include the analog high speed integrated circuit for the wireless LAN applications. Mr. Youssef is the recipient of the Mobinil Telecommunication Inc. Pre-master Fellowship in 1999. He also received the Young Scientist award at the Maastricht General Assembly of the International Union of Radio Science in 2002 and an Honorable Mention at 2003 in the Symposium of the Microelectronics Research & Development in Canada, Montreal. Mr. Youssef received the Gordon Lewis Hedberg Doctoral Scholarship in 2005.     

CBF beta-SMMHC, expressed in M4Eo AML, reduced CBF DNA-binding and inhibited the G1 to S cell cycle transition at the restriction point in myeloid and lymphoid cells

CBF beta-SMMHC is expressed from the inv(16) chromosome in M4Eo AML. Mice lacking CBF subunits or expressing the CBF beta-SMMHC or AML1-ETO oncoproteins failed to develop definitive hematopoiesis. To investigate these effects on hematopoiesis, we expressed CBF beta-SMMHC from the metallothionein promoter, in both 32D cl3 myeloid cells and Ba/F3 B-lymphoid cells. Addition of zinc increased CBF beta-SMMHC levels more than tenfold, with higher levels evident in Ba/F3 lines. Levels obtained in 32D cl3 cells were similar to those of endogenous CBF beta. Indirect immunofluorescence revealed zinc-inducible speckled, nuclear staining in Ba/F3 cells and diffuse nuclear staining in 32D cl3 cells. CBF beta-SMMHC reduced endogenous CBF DNA-binding fivefold in both cell types, increased cell generation time 1.9-fold, on average, in 32D cl3 cells and 1.5-fold in Ba/ F3 cells and decreased tritiated thymidine incorporation into DNA correspondingly. CBF beta-SMMHC increased the proportion of cells in G1 1.7-fold, on average, in 32D cl3 and Ba/F3 cells, and decreased the proportion of cells in S phase by a similar degree. CBF beta-SMMHC induced a marked increase in hypophosphorylated Rb, but did not alter IL-3 Receptor alpha or beta subunit levels. Neither apoptosis nor 32D differentiation was induced by zinc in IL-3 in these lines. Induction of CBF beta-SMMHC in 32D cl3 cells did not inhibit their differentiation to neutrophils or their expression of myeloperoxidase mRNA in G-CSF, and did not produce an eosinophilic phenotype. Additional, proliferative genetic changes in M4eo AMLs might potentiate inhibition of differentiation by CBF beta-SMMHC by allowing its increased expression.     

Pulmonary and allergy subspecialty care in adults with asthma. Treatment, use of services, and health outcomes

This study examined the relationship of several maternal variables to the duration of exclusive breast-feeding and the total duration of breast-feeding, along with attitudes, perceptions, and beliefs about breast-feeding among women living in poor neighborhoods of Managua, Nicaragua. The field work was carried out in December 1992 and January 1993 using qualitative and quantitative methods. A structured questionnaire was administered in interviews with 556 mothers of children under 12 months of age, and meetings of four directed discussion groups were held, in which a total of 20 women participated. At one week of age, almost all the children of the mothers who were surveyed had been breast-fed, but only 45% had been exclusively breast-fed. At 12 weeks old, 30% were already completely weaned. The discussion groups revealed the coexistence of positive opinions about both breast-feeding and bottle-feeding. However, exclusive breast-feeding was considered harmful for the mother, and breast milk was not thought to be sufficient nourishment for the child. Previous experience was strongly related to the duration of exclusive breast-feeding and to total breast-feeding duration. Attitudes, social support, and work situation were important factors influencing the total length of time women breast-fed a child. In general, the results obtained through the interviews and in the discussion groups were in agreement and showed that the elements needed to promote exclusive breast-feeding were social support, a favorable community environment, and policies that dealt with problems faced by working mothers. The complementary research methods were useful for obtaining information about the relative importance of different factors that determine the duration of breast-feeding and for understanding that practice in greater depth from the mother's point of view.     

A new scalable multicast routing algorithm for interactive real-time applications

Quality of service (QoS) provisioning generally assumes more than one QoS measure that implies that QoS routing can be categorized as an instance of routing subject to multiple constraints: delay jitter, bandwidth, cost, etc. We study the problem of constructing multicast trees to meet the QoS requirements of real-time interactive applications where it is necessary to provide bounded delays and bounded delay variation among the source and all destinations while keeping overall cost of the multicast tree low. The main contribution of our work is a new strategy for constructing multiconstrained multicast trees. We first derive mathematically a new delay-variation estimation scheme and prove its efficiency. Thereafter, we propose a simple and competitive (in terms of running time) heuristic algorithm, for delay and delay variation constrained routing problem based on the proposed delay-variation estimation scheme and using the Extended Prim-Dijkstra tradeoffs’ algorithm. Our contribution also extends previous works in providing some properties and analyses of delay bounded paths satisfying delay variation constraints. Extensive simulation results show that our algorithm outperforms DVDMR in terms of multicast delay variation with the same time complexity as DVDMR.     

Structure and properties of the cell nucleus chromatin in the brain of rats exposed to gamma-radiation]

Exposure of rats to radiation in a dose of 1 Gy changes sensitivity of chromatin in the cerebral cortex cells to the action of DNAase 1, which promotes an increase of the DNA hydrolysis level and content of dissolved chromatin fractions. A day after irradiation the chromatin structure restores only partially and then (7-30 days after irradiation) it passes into a new, less compact state. The irradiation changes the chromatin ability to aggregation in the presence of Mg2+ and spermidine.     

Dyeing of cationised cotton with acid dyes

The production of cationised cotton fabrics, via Sandene 8425, 1, 1-dimethyl-3-hydroxy azetidinium chloride and 1, 1-diethyl-3-hydroxy azetidinium chloride, greatly enhances the exhaustion and fixation of acid dyes. This paper shows that these dyes can be used in the absence of salt in a neutral medium. Wash fastness, light fastness and other properties were studied.     

Generalized Thermoelastic Infinite Layer Subjected to Ramp-Type Thermal and Mechanical Loading under Three Theories—State Space Approach

A problem of thermoelastic interactions in an elastic infinite layer 0 ≤ x ≤ h with an elevated temperature field arising from ramp-type heating and loading has been constructed. The governing equations are written in a unified system from which the field equations for coupled thermoelasticity as well as for generalized thermoelasticity can be easily obtained as particular cases. Due attention has been paid to the finite rise times of temperature and stress. The problem has been solved analytically by using a state-space approach. Solutions from the derived analytical expressions have been computed for a specific situation. The solution for the half-space when (h → ∞) has been found also. Numerical results for the temperature distribution and thermal stress are represented graphically. A comparison was made among the results predicted by the theories.     

Influence du dodecyl sulfate de sodium sur la tension interfaciale du système biphasé butanol-eau

Interfacial tension between the two phases formed by the partially miscible system composed of butanol-1 and water has been investigated. Using the method of drop volume tensiometry, the effect of a surface-active agent, sodium dodecyl sulfate, on interfacial tension was investigated. Addition of surfactant to the butanol-rich phase, which forms the droplet, has been found to bring about a decrease in interfacial tension the magnitude of which was influenced by the time of contact. When the surfactant was added to the aqueous (bulk) phase, the decrease in interfacial tension was greater and it was equal to that observed when the surfactant was added to both phases. It is concluded that the orientation of phases in the instrument has an important effect on the apparent interfacial tension observed.