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91.
An EPR investigation of-irradiated PETN (Pentaerythritol Tetranitrate) was conducted. Low temperature irradiation accumulates at least two different radicals. The more stable radical is the NO2 radical presumably produced by photon cleavage of the NO2 groups and/or by radical reactions in excited states resulting in detached NO2. The spectrum of the more reactive radical is obtained by computer substraction. Decay kinetics are proposed for the NO2 and the unknown highly reactive radical. 相似文献
92.
Role of primate medial vestibular nucleus in long-term adaptive plasticity of vestibuloocular reflex
1. Fifteen hundred and thirty cells were recorded in the medial vestibular nucleus (MVN) of alert monkeys whose vestibuloocular reflex (VOR) had been adapted to one of two kinds of spectacles. The "high-gain" sample was recorded from monkeys that had worn 2.0 x telescopic spectacles; the gain of the VOR in the dark (eye velocity divided by head velocity) was greater than 1.5. The "low-gain" sample was recorded from monkeys that had worn goggles providing a visual field that was fixed with respect to the freely turning head; the gain of the VOR was less than 0.4. 2. Cells showing modulation of firing rate related to imposed head velocity were grouped into four categories: pure vestibular (10), vestibular-plus-saccade (10), vestibular-plus-position (10), and vestibular-plus-head/body (24). Sensitivity to head velocity was measured from averaged responses to sinusoidal, 0.4-Hz whole-body oscillation in the horizontal plane. Almost all cells (98%) having increased firing during ipsilateral head rotation received inputs from the horizontal semicircular canals. Conversely, 82% of cells having increased firing during contralateral head rotation received inputs from the vertical canals. 3. There were no statistically significant differences in resting discharge rate, phase shift, or sensitivity to head velocity between the high- and low-gain samples of any of the cell types. Nonetheless, there was a consistent tendency, evident in all the functionally defined cell groups, for the sensitivity to be about 20% greater in the high-gain samples. However, this difference is small by comparison with the fourfold difference in VOR gain. 4. Detailed scrutiny of the response properties of individual cells suggested that the small differences in sensitivity reflect small changes distributed throughout the population, rather than large and potentially significant changes within a small sub-population. 5. Our data indicate that large, adaptive changes in the gain of the VOR are accompanied by only minor changes in the vestibular sensitivity and no changes in the phase shift or resting discharge rates of cells in the MVN. It remains possible that large changes in vestibular sensitivity occurred in cells we did not sample or in subgroups we could not identify. We argue that this is unlikely and that the major changes underlying VOR plasticity occur after the first central synapse in the VOR pathways. 相似文献
93.
Raitt L. Miles 《Lakes & Reservoirs: Research and Management》2012,17(4):275-289
The present study examined spatial, seasonal and depth variations in phytoplankton biomass and primary production (PP), compared with those reported for other tropical African lakes, to determine whether or not measured phytoplankton changes might be linked to climate warming. The biomass of three major phytoplankton classes (Cyanophyceae; Chlorophyceae; Bacillariophyceae) and net PP were measured during the midwinter and midsummer at six different depths at 35 sampling sites distributed across the lake’s five basins. A more rigorous sampling regime was used in the fifth basin, with phytoplankton biomass and PP rates measured every second month over a 24 month period at six different depths at ten sampling sites located in riverine and lacustrine waters. Cyanophyceae, which displayed a gradient of decreasing biomass from Basins 2 to 5, contributed 69% of the total phytoplankton biomass in the lake’s five basins during summer. This percentage was approximately four times greater than that contributed by the Bacillariophyceae and about ten times greater than that contributed by the Chlorophyceae. During winter, Bacillariophyceae biomass was equivalent to that of the Cyanophyceae, but displayed an opposing gradient of increasing biomass from Basins 1 to 3, with a subsequent biomass decline from Basins 3 to 5. Chlorophyceae exhibited no distinct biomass gradient across the five lake basins, being undetectable during winter. The biomass of all three phytoplankton classes and the net PP varied in magnitude and direction monthly between the lacustrine and riverine waters, with increasing water depth and with no distinct seasonal patterns being evident. The monthly variations in biomass were related to the thermal stratification cycle, hydrological gradients and the extent of water mixing, being similar to those reported for other tropical African lakes. It is noteworthy that total phytoplankton biomass and PP in Lake Kariba have declined by about 95% and 50%, respectively, since the 1980s. These declines correspond to an upward shift in the depth of the thermocline, associated with an average temperature increase of 1.9 °C and a 50% reduction in the depth of the euphotic zone, since the 1960s. 相似文献
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Two new methods of activation were developed to graft enzymes on collegen films. They involved chemical modifications of surface groups of collagen either by Woodward's reagent "K" or by EDC, a water-soluble derivative of carbodiimide. EDC was a better coupling agent and a detailed study was conducted with this agent. It could be used either in a global method of activation and coupling, or in a two-step procedure of activation of collagen, followed by spontaneous coupling of enzyme. All enzymes tested were successfully bound: malate dehydrogenase, lactate dehydrogenase, aspartate aminotransferase, urease, creatine kinase, hexokinase. The influence on the yield of grafted enzyme, of pretreatment of films, time and temperature of EDC activation, concentration of EDC and enzyme, protecting agents was studied. Stability of enzyme activity on storage was greatly increased after grafting. A co-grafted dual system creatine kinase/heoxkinase, was achieved which exhibited a good efficiency. A striking renaturing process at 0-4degreesC after thermal denaturation, was observed with hexokinase. 相似文献
96.
1. The 129 MHz (non-proton decoupled) and 36.4 MHz (proton decoupled) 31P NMR spectra arising from unsonicated aqueous dispersions of well defined species of phospholipid have been investigated. The phospholipids employed (and the parameters varied) include phosphatidylcholine (temperature), phosphatidylethanolamine (temperature), phosphatidic acid (temperature and p2H) and phosphatidylglycerol (temperature, p2H and Ca2+ (or Mg2+)) concentration. 2. At p2H = 7 the 31 P MNR spectra arising from saturated species of phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol become progressively broader as the temperature is reduced below the phase transition, demonstrating reduced motion in the phosphate region of the polar headgroup. 3. In the liquid crystalline state at p2H = 7 the molecular dipolar order parameters obtained for saturated species of phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol and very similar, and are independent of the acyl chain length for species derived from lauric and myristic acid. Thus the motion in the methylene-phosphate-methylene region is similar for these different liquid crystaline phospholipid species. 4. The 31 P NMR spectra of aqueous dispersions of 14:0/14:0 phosphatidic acid display anomalous temperature and p2H dependences. The effective chemical shift anistropy (delta v CSA EFF) at 5 degrees C varies from 71 ppm at p2H = 8.5 to 38 ppm at p2H = 2.5. Further, the motion in the phosphate region is relatively insensitive to the gel or liquid crystalline nature of the hydrocarbon chains. 5. The addition of 40 mol% Ca2+ (or Mg2+) to saturated species of phosphatidylglycerol causes an increase of approx. 20 degrees C in the hydrocarbon phase transition temperature as indicated by 31 P NMR. Equimolar concentrations of Ca2+ increase the transition temperature by approx. 70 degrees C, and no 31P NMR signal could be observed for the very condensed precipitate formed below this temperature. In the liquid crystalline state the motion in the phosphate region of the polar headgroup is not significantly affected by the presence of Ca+ or Mg2+. 6. The 31P NMR spectra obtained from 18 : 1c/18 : 1c phosphatidylethanolamine are consistent with a phase transition from a lamellar to an hexagonal (HII) phase in the region 10-15 degrees C. 7. The observed narrowing of the 31 P NMR spectra of aqueous dispersions of phospholipids as the temperature is raised toward the hydrocarbon transition temperature is discussed in terms of the "pretransition" observed in calorimetric studies. 相似文献
97.
Miles Felicity J.; Everitt Barry J.; Dalley Jeffrey W.; Dickinson Anthony 《Canadian Metallurgical Quarterly》2004,118(6):1331
Food-reinforced conditioned activity and instrumental responding were measured in rats after 50 weeks of continuous access to a cocaine-saccharin solution in their home cages. The elevation of conditioned activity produced by acute access to the cocaine-saccharin solution in the home cage during testing was abolished by long-term preexposure to the cocaine solution, an effect that was reversed by systemic administration of cocaine immediately prior to testing. By contrast, chronic cocaine preexposure enhanced instrumental responding for both cocaine-sucrose and pure sucrose solutions. These results support the idea that long-term cocaine exposure enhances subsequent reinforcement. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
98.
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100.
Miles Glendinning 《The Journal of Architecture》2016,21(4):630-650
Following the downfall of fascism in World War II, the old alliance of modernism and socialism dispersed, as architecture was sucked into the confrontation of the Cold War. In response, a new type of international architectural organisation emerged, shaped not by utopian idealism but by cultural diplomacy and international goodwill, along with consensual policy objectives, such as advancement of the architect's professional status. The most influential international organisation of architects — the ‘United Nations’ of architecture — was the Union Internationale des Architectes/International Union of Architects (UIA/IUA), a Paris-based society headed by the Hungarian-born Pierre Vago. Although only a small organisation, like many NGOs in symbolic areas of cultural activity, the UIA exploited the polarisations of the Cold War, carving out an influential mediating niche for itself. This paper focuses on the UIA congresses during the period of greatest Cold War instability — Moscow, 1958; London, 1961; Havana/Mexico City, 1963 — showing how the first and third projected the Union into hotbeds of Cold War instability. 相似文献