Hypoxia-ischemia induces a rapid elevation of ubiquitin conjugate levels and ubiquitin immunoreactivity in the immature rat brain

Postnatal rats at 7 and 21 days of age were subjected to unilateral hypoxia-ischemia (H/I) by right carotid artery ligation followed by 1.5 to 2 hours of hypoxia (8% oxygen). Brains were frozen at specific intervals of recovery from 0 to 24 hours. Western blots of samples of right and left forebrain were immunodeveloped with a monoclonal antibody specific for ubiquitin, RHUb1. An elevation of ubiquitin conjugate levels in the right compared with the left forebrain of 7-day-old animals was detectable immediately following H/I and increased by close to 60% of control level within 1 hour of recovery. The conjugate immunoreactivity remained at this level for 6 hours but had declined to control levels by 24 hours of recovery. No such increase was observed in response to hypoxia alone. Similar changes were observed in samples from the 21-day-old rat brain. However, the elevation of ubiquitin conjugate levels was of slower onset and persisted longer than observed for the 7-day-old animals. Immunocytochemical studies of brain fixed by immersion in formaldehyde/acetone/methanol showed that ubiquitin-like immunoreactivity was increased in the right, but not left, cerebral cortex and hippocampus of animals subjected to H/I. The data suggest that elevated ubiquitination may represent a neuroprotective response to H/I.     

Integrator backstepping techniques for the tracking control ofpermanent magnet brush DC motors

An integrator backstepping technique is introduced for the design of high-performance motor controllers. The approach is applied to the design of embedded computed torque and output feedback controllers for permanent magnet brush DC (BDC) motors. The proposed controllers are simulated and experimentally verified on a user-developed digital signal processor (DSP) based data acquisition and control (DAC) system. Although the BDC motor is well researched, the results motivate extensions of the proposed techniques to the development of similar controllers for more complex electromechanical systems, such as separately excited DC, permanent magnet stepper, brushless DC, switched reluctance, and AC induction servo motors     

Novel Thermally Conductive Thermoplastic/Ceramic Composite Foams

Multifunctional materials that are lightweight and thermally conductive but electrically insulating are important for modern electronics, computer, and telecommunication technologies. Here, a novel foam structure of a polymer/matrix composite filled with ceramic platelets with improved thermal conductivity is reported. Such improvement is caused by the stress‐induced alignment of thermally conductive fillers in the cell wall of the plastic foam. The foam structure is very promising for use as a lightweight electronic packaging material owing to its light weight, thermal conduction ability, electrical insulation, and good processability.

     

A Structural Rationale for N-Methylbicuculline Acting as a Promiscuous Competitive Antagonist of Inhibitory Pentameric Ligand-Gated Ion Channels

Bicuculline, a valued chemical tool in neurosciences research, is a competitive antagonist of specific GABA_A receptors and affects other pentameric ligand-gated ion channels including the glycine, nicotinic acetylcholine and 5-hydroxytryptamine type 3 receptors. We used a fluorescence-quenching assay and isothermal titration calorimetry to record low-micromolar dissociation constants for N-methylbicuculline interacting with acetylcholine-binding protein and an engineered version called glycine-binding protein (GBP), which provides a surrogate for the heteromeric interface of the extracellular domain of the glycine receptor (GlyR). The 2.4 Å resolution crystal structure of the GBP:N-methylbicuculline complex, sequence and structural alignments reveal similarities and differences between GlyR and the GABA_A receptor–bicuculline interactions. N-methylbicuculline displays a similar conformation in different structures, but adopts distinct orientations enforced by interactions and steric blocks with key residues and plasticity in the binding sites. These features explain the promiscuous activity of bicuculline against the principal inhibitory pentameric ligand-gated ion channels in the CNS.     

Spots before the eyes: Can ink jet printers match expectations?†

The mechanism by which the coloured image is formed by digital ink jet printing is very different from that of conventional screen printing methods and success depends on the use of sophisticated software and hardware. Some commercially available systems are listed in this paper and the basic principles of the standardisation of scanners, monitors and jet printers given. Ink jet printing machines at present available for textiles are reviewed and their limitations with regard to dye selection, shade gamuts and reproducibilty are quantified. Repeatability and shade reproducibility tests of digitally printed textiles are reported showing that improvements are required in the transformation of pattern data to the printed output.     

Structural and functional analogy between pneumolysin and proaerolysin

Pneumolysin and proaerolysin are bacterial toxins that form pores in host cells by oligomerization. We propose that they may have similar structures despite a poor sequence identity. The crystal structure of proaerolysin reveals a protein composed of four domains, arranged in the shape of an elongated comma. Electron microscopy of the pneumolysin monomer shows a similar arrangement of domains. The sequence of pneumolysin recognizes the template of proaerolysin from a library of protein folds. A three-dimensional model of pneumolysin has been constructed by the comparative approach using the structure of proaerolysin. This model, together with results on the activity of site- specific mutants and the positions of antigenic sites, has been used to propose functional roles of individual domains.      

Biosynthesis and synthesis of natural colours

In nature, the detection of colours requires an organism having some type of eye with a retina and two or three types of photoreceptor connected to a nervous system, which can interpret the signals received. Evidence that certain simple organisms were coloured 1 billion years ago, and some more advanced creatures, which could have possessed eyes a few hundreds of million years ago, is exemplified. A vast array of chemicals essential to life are produced by living organisms and their biosynthesis depends upon individual genetic patterns, which determine the enzyme catalysts involved. Plants photosynthesise many pigments, which are essential for them to maximise the absorption of energy from the sun, while others offer protection from any harmful radiation. Such pigments, for example chlorophylls and the carotenoids, flavonoids and betalains, have traditionally been used as natural dyes, food colorants and medicines. This review compares the chemical processes involved in their biosynthesis and the laboratory methods adopted to confirm their chemical structure. Some engineered biosynthetic methods are now used for commercial production of natural colours and these methods may involve the controversial use of genetic engineering.     

Quantification of tool wear using white light interferometry and three-dimensional computational metrology

Although several wear modes can result from machining, the most common tend to be what are referred to as flank wear and crater wear. Flank wear can be easily measured directly from images of a worn cutting tool, and this is the typical method used to quantify the condition of a tool. On the other hand, crater wear is difficult to quantify, and thus has typically been treated in a qualitative manner. The inability to characterize and compare the two wear modes in a quantitative way is an increasingly important problem as the precision of machining operations improves and cutting moves almost exclusively to the nose radius of cutting tools. This paper introduces a new approach to this problem by proposing a technique to quantify both wear modes for direct comparison. The technique measures the volumetric wear loss in the two regions by comparing three-dimensional wear data obtained by white light interferometry with ideal representations of unworn cutting tools. The resulting wear measurements are compared and related to changes in the cutting process, specifically increases in cutting forces and changes in the topography of machined surfaces.     

Cell synchrony and periodic behaviour in yeast populations

The underlying basis for the behaviour of a population of cells is the cell growth and division cycles of the individual cells. Under certain special circumstances, oscillations in cell cultures can be observed that are a reflection of metabolic oscillations, such as in glycolysis, or cell cycle oscillations from a partially synchronized population. These oscillations can sometimes occur spontaneously, as in the chemostat culture of Saccharomyces cerevisiae at low dilution rates, or can be induced using chemical blockers or entrainment by a periodic nutrient environment. Continuous methods for inducing a synchronized culture include the pulsed chemostat, continuous phasing and self-cycling fermentation. Results obtained by studying both spontaneously oscillating cultures and synchronized cultures have provided insight into the mechanisms responsible for the progress and control of the cell cycle as well as the intimate relationship that exists between the cellular dynamics and the dynamics of the environment.