Interactions of EGF, Wnt and HOM-C genes specify the P12 neuroectoblast fate in C. elegans

We investigate how temporal and spatial interactions between multiple intercellular and intracellular factors specify the fate of a single cell in Caenorhabditis elegans. P12, which is a ventral cord neuroectoblast, divides postembryonically to generate neurons and a unique epidermal cell. Three classes of proteins are involved in the specification of P12 fate: the LIN-3/LET-23 epidermal growth factor signaling pathway, a Wnt protein LIN-44 and its candidate receptor LIN-17, and a homeotic gene product EGL-5. We show that LIN-3 is an inductive signal sufficient to promote the P12 fate, and the conserved EGF signaling pathway is utilized for P12 fate specification; egl-5 is a downstream target of the lin-3/let-23 pathway in specifying P12 fate; and LIN-44 and LIN-17 act synergistically with lin-3 in the specification of the P12 fate. The Wnt pathway may function early in development to regulate the competence of the cells to respond to the LIN-3 inductive signal.     

Hydroxylation of Progesterone and Its Derivatives by the Entomopathogenic Strain Isaria farinosa KCh KW1.1

Progesterone biotransformation is worth studying because of the high industrial value of its derivatives. This study investigated the catalytic ability of the entomopathogenic filamentous fungus strain Isaria farinosa KCh KW1.1 to transform progesterone derivatives: 11α-hydroxyprogesterone, 17α-hydroxyprogesterone, 16α,17α-epoxyprogesterone and pregnenolone. In the culture of Isaria farinosa KCh KW1.1, 11α-hydroxyprogesterone was effectively transformed into only one product: 6β,11α-dihydroxyprogesterone. Transformation of 17α-hydroxyprogesterone gave three hydroxy derivatives: 6β,17α-dihydroxyprogesterone, 12β,17α-dihydroxyprogesterone and 6β,12β,17α-trihydroxyprogesterone. Two products: 6β-hydroxy-16α,17α-epoxyprogesterone and 6β,11α-dihydroxy-16α,17α-epoxyprogesterone, were obtained from the 16α,17α-epoxyprogesterone transformation. We isolated two compounds from the biotransformation medium with pregnenolone: 11α-hydroxy-7-oxopregnenolone and 5α,6α-epoxy-3β,11α-dihydroxypregnan-7,20-dione. In this study, we observed only mono- and dihydroxy derivatives of the tested substrates, and the number of obtained products for each biotransformation did not exceed three.     

Molecular Framework of Mouse Endothelial Cell Dysfunction during Inflammation: A Proteomics Approach

A key aspect of cytokine-induced changes as observed in sepsis is the dysregulated activation of endothelial cells (ECs), initiating a cascade of inflammatory signaling leading to leukocyte adhesion/migration and organ damage. The therapeutic targeting of ECs has been hampered by concerns regarding organ-specific EC heterogeneity and their response to inflammation. Using in vitro and in silico analysis, we present a comprehensive analysis of the proteomic changes in mouse lung, liver and kidney ECs following exposure to a clinically relevant cocktail of proinflammatory cytokines. Mouse lung, liver and kidney ECs were incubated with TNF-α/IL-1β/IFN-γ for 4 or 24 h to model the cytokine-induced changes. Quantitative label-free global proteomics and bioinformatic analysis performed on the ECs provide a molecular framework for the EC response to inflammatory stimuli over time and organ-specific differences. Gene Ontology and PANTHER analysis suggest why some organs are more susceptible to inflammation early on, and show that, as inflammation progresses, some protein expression patterns become more uniform while additional organ-specific proteins are expressed. These findings provide an in-depth understanding of the molecular changes involved in the EC response to inflammation and can support the development of drugs targeting ECs within different organs. Data are available via ProteomeXchange (identifier PXD031804).     

Bars on blocks: A cellular automata model of crime and liquor licensed establishment density

Criminologists have extensively researched the problems generated by licensed establishments. Violent offending and disorderly behavior resulting from pubs, taverns, dance clubs and bars are of particular interest to this field of study. The relative density of these liquor establishments has been found to be associated with the level of violence and disorder in surrounding areas. A complex systems approach can be used to further understand the dynamic interplay between licensed establishments, violent offending and disorder, and urban planning decisions. The model presented here utilizes cellular automata as the mathematical framework to view the varying impact of liquor licensing density on crime. This study uses a sample of liquor establishments and crime data from the City of Vancouver in British Columbia. The cellular automata model incorporates transition rules which govern the change of city blocks from low-risk blocks to high-risk blocks. The results represented by a 50 × 50 cellular grid show that high-risk blocks multiply when liquor licenses are grouped. Two scenarios are presented to contrast the impact of grouping high-risk blocks which contain more liquor establishments and dispersing such blocks. A third scenario demonstrates how increasing the positive influence in a grouped scenario stops high-risk blocks from taking over the entire grid. Future iterations of this model will incorporate census data, public transportation data, land use data and entertainment districts from other cities to further analyze the effect of licensed establishments on the distribution of crime.     

Achieving Optimal Inter-Node Communication in Graph Partitioning Using Random Selection and Breadth-First Search

Processing large graph datasets represents an increasingly important area in computing research and applications. The size of many graph datasets has increased well beyond the processing capacity of a single computing node, thereby necessitating distributed approaches. As these datasets are processed over a distributed system of nodes, this leads to an inter-node communication cost problem that negatively affects system performance. Previously proposed algorithms implemented breadth-first search (BFS) for graph searching and focused on the execution, parallel performance and not the communication. In this paper a new methodology is proposed that combines BFS with random selection in order to partition large graph datasets and effectively minimize inter-node communication. The new method is discussed and applied to the single-source shortest path and PageRank algorithms using three graphs that are representative of real-world scenarios. Experimental results show that graph inter-node communication for canonical graphs representative of real-world data is improved up to 42 % in case of Powerlaw graph, up to 27 % in case of Random near K-regular graph (with low degree), and up to 7 % in case of Random near K-regular graph (with high degree).     

居民区雷电保护系统的试验:火箭触发雷电和电流冲击发生器试验数据的比较

We present a comparison of data obtained during testing of lightning protective system of a residential structure in rocket-triggered lightning experiment at the International Center for Lightning Research and Testing(ICLRT)at Camp Blanding,Florida,and current surge generator experiment at Rzeszow University of Technology in Poland.Three different configurations of LPS were tested in Poland with the dc grounding resistances of the entire system 4.09 Ω(LPS 1a),1.65 Ω(LPS 1b),and 2.88 Ω(LPS 2).For LPS 1a with three ground rods the value of the peak current entering the electrical circuit neutral was about 56% of the injected current peak,and for LPS 1b with two additional ground rods and two 5 m long buried horizontal conductors it was about 16%.For LPS 2 with five ground rods interconnected by a buried loop conductor this ratio was 21%.The current waveshapes in the ground rods differed from the injected current waveshapes and the current waveshapes in other parts of the test system,especially,for poorer-grounding LPS 1a.The surge-generator results are consistent with those of triggered-lightning experiments at Camp Blanding,Florida(DeCarlo et al.,2008 [2]).     

Entanglement energetics in the ground state

Abstract

We show how many-body ground state entanglement information may be extracted from sub-system energy measurements at zero temperature. A precise relation between entanglement and energy fluctuations is demonstrated in the weak coupling limit. Examples are given with the two-state system and the harmonic oscillator, and energy probability distributions are calculated. Comparisons made with recent qubit experiments show this type of measurement provides another method to quantify entanglement with the environment.     

Effectiveness of traffic light vs. boom barrier controls at road–rail level crossings: A simulator study

Although collisions at level crossings are relatively uncommon occurrences, the potential severity of their consequences make them a top priority among safety authorities. Twenty-five fully-licensed drivers aged between 20 and 50 years participated in a driving simulator study that compared the efficacy, and drivers’ subjective perception, of two active level crossing traffic control devices: flashing lights with boom barriers and standard traffic lights. Because of its common usage in most states in Australia, a stop sign-controlled level crossing served as the passive referent. Although crossing violations were less likely at the level crossings controlled by active devices than at those controlled by stop signs, both kinds of active control were associated with a similar number of violations. Further, the majority (72%) of drivers reported preferring flashing lights to traffic lights. Collectively, results indicate that the installation of traffic lights at real-world level crossings would not be likely to offer safety benefits over and above those provided already by flashing lights with boom barriers. Furthermore, the high rate of violations at passively controlled crossings strongly supports the continued practice of upgrading level crossings with active traffic control devices.     

优化建筑物内无线网络

正1引言移动数据流量的很大部分发生在室内。确保室内高密度用户的最佳网络质量显得特别重要。为了规划、实施和优化室内无线网络,首选必须对所有广泛使用的网络的无线信道质量进行可靠和全面评估。极其轻便和紧凑的路测扫描仪非常适合这一目标。甚至采用背包系统就能并行测量所有相关标准,因此极大缩短了测量时间。因为宽带市场上涌现出智能手机和平板电脑,移动宽带数据业务应用急速增加。仅2012年,全球移动数据