Shear Strengthening Masonry Panels with Sheet Glass-Fiber Reinforced Polymer

This paper investigates strengthening masonry walls using glass-fiber reinforced polymer (GFRP) sheets. An experimental research program was undertaken. Both clay and concrete brick specimens were tested, with and without GFRP strengthening. Single-sided strengthening was considered, as it is often not practicable to apply the reinforcement to both sides of a wall. Static tests were carried out on six masonry panels, under a combination of vertical preload, and in-plane horizontal shear loading. The mechanisms by which load was carried were observed, varying from the initial, uncracked state, to the final, fully cracked state. The results demonstrate that a significant increase of the in-plane shear capacity of masonry can be achieved by bonding GFRP sheets to the surface of masonry walls. The experimental data were used to assess the effectiveness of the GFRP strengthening, and suggestions are made to allow the test results to be used in the design of sheet GFRP strengthening for masonry structures.     

Three-Dimensional Numerical Modeling of Mixing at River Confluences

Lateral mixing of a pollutant is considered as a slow process that is usually complete within 100–300 river widths. Recent studies on flow dynamics at river confluences revealed that lateral mixing can be markedly enhanced when the tributary channel is shallower than the main channel. This study uses a three-dimensional model to examine mixing processes immediately downstream of confluences as well as further downstream in the mainstream. Simulations are presented for a concordant and discordant laboratory junction and a field confluence for a low and a high flow condition. The decrease in standard deviation at a cross section of a tracer over a distance of 5 channel widths is 30% for discordant beds but only 10% for concordant beds in the laboratory simulation. At the natural site, bed discordance is more important at the low flow than at the high flow with corresponding decreases in the standard deviation of 31 and 18% over 3.5 channel widths. Mixing is completed after a distance of 25 and 37 channel widths for the low and high flow conditions, respectively. Further downstream, mixing is mainly affected by planform curvature of the channel.     

Prelimbic cortex specific lesions disrupt delayed-variable response tasks in the rat.

The role of the prelimbic cortex (PL) in rats was investigated with excitotoxic lesions. PL lesions altered the alternation scores in spontaneous and reinforced spatial delayed-alternation tasks. PL lesions induced a delay in conditioning under a temporal go/no-go alternation schedule but not under a continuous food-reinforcement schedule in a runway. PL lesions had no effect on the acquisition of a standard radial-arm-maze task nor on a fixed-goal location task but disrupted the acquisition of a variable-goal location task in a radial-arm maze. The present results indicate that PL lesions replicated most of the behavioral deficits obtained with larger prefrontal lesions. PL lesions disrupted the acquisition of delayed-variable response tasks while leaving unaffected fixed-response tasks. These results are discussed in relation with a working-memory, a response-selection, and an attentional hypothesis. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Care transitions in the outpatient surgery preoperative process: facilitators and obstacles to information flow and their consequences

Patient care transitions have been shown to be critical points at which failure as well as recovery from potential failure may occur. The purpose of this research was to identify transitions in patient care and the flow of associated information at different steps in the outpatient surgery preoperative care process and, in turn, attempt to identify breakdowns in the information flow process and their ramifications. A study of one organization’s preoperative process for outpatient surgery was conducted, employing four means of data collection to gather information on preoperative work processes: employee shadowing, patient shadowing, clinic observation, and dictated feedback. Various facilitators and obstacles in information flow were found to be present in the preoperative care process. Obstacles often resulted in negative consequences for healthcare providers and patients. Helping care providers understand how their actions affect the various elements of the preoperative process, through improved awareness, may be one way to improve information flow problems within the outpatient surgery process.     

Reduction efficiency of the neurotoxin β‐ODAP in low‐toxin varieties of Lathyrus sativus seeds by solid state fermentation with Aspergillus oryzae and Rhizopus microsporus var chinensis

Solid state fermentation of several low‐toxin varieties of grass pea (Lathyrus sativus L) seeds with Aspergillus oryzae and Rhizopus microsporus var chinensis removed the neurotoxin β‐ODAP (3‐N‐oxalyl‐L ‐2,3‐diaminopropanoic acid) to a considerable degree from the seed meal. The detoxification efficiency was statistically significant and ranged from 52.4% (p < 0.01) to 82.2% (p < 0.001), which was lower than for a high‐toxin variety processed by the same fermentation procedure (94.8%, p < 0.001). While the content of β‐ODAP decreased, those of other free protein amino acids, especially glutamic acid, histidine, threonine, tyrosine, valine and lysine, increased dramatically in the fermented seeds. Efforts to remove the neurotoxin from Lathyrus sativus either by breeding or by food processing to obtain toxin‐free grass pea seeds have been made worldwide for several decades. The efficiencies of various reported processing methods are summarised and compared. © 2000 Society of Chemical Industry     

Intravesicular Genomic DNA Enriched by Size Exclusion Chromatography Can Enhance Lung Cancer Oncogene Mutation Detection Sensitivity

Extracellular vesicles (EVs) are cell-derived structures surrounded by a lipid bilayer that carry RNA and DNA as potential templates for molecular diagnostics, e.g., in cancer genotyping. While it has been established that DNA templates appear on the outside of EVs, no consensus exists on which nucleic acid species inside small EVs (<200 nm, sEVs) are sufficiently abundant and accessible for developing genotyping protocols. We investigated this by extracting total intravesicular nucleic acid content from sEVs isolated from the conditioned cell medium of the human NCI-H1975 cell line containing the epidermal growth factor (EGFR) gene mutation T790M as a model system for non-small cell lung cancer. We observed that mainly short genomic DNA (<35–100 bp) present in the sEVs served as a template. Using qEV size exclusion chromatography (SEC), significantly lower yield and higher purity of isolated sEV fractions were obtained as compared to exoEasy membrane affinity purification and ultracentrifugation. Nevertheless, we detected the EGFR T790M mutation in the sEVs’ lumen with similar sensitivity using digital PCR. When applying SEC-based sEV separation prior to cell-free DNA extraction on spiked human plasma samples, we found significantly higher mutant allele frequencies as compared to standard cell-free DNA extraction, which in part was due to co-purification of circulating tumor DNA. We conclude that intravesicular genomic DNA can be exploited next to ctDNA to enhance EGFR T790M mutation detection sensitivity by adding a fast and easy-to-use sEV separation method, such as SEC, upstream of standard clinical cell-free DNA workflows.     

Reaction of α, ω-dicarboxy polyamides in the melt: synthesis of a model

Summary The synthesis of N-dodecanoyl-11-aminoundecanoic acid as a model for carboxylic polyamides 11 is described. The solubilization reaction of these polyamides with trifluoroacetic anhydride is studied by ¹H and ¹³C NMR spectroscopy. The resulting soluble trifluoroacetylated compounds are stable enough to allow an easy GPC study in CH₂Cl₂ on routine instument and columns. The participation of the residual dodecanoic acid in the polycondensation equilibrium of carboxylic polyamides has been established.     

Molecular Properties of Human Guanylate Cyclase-Activating Protein 3 (GCAP3) and Its Possible Association with Retinitis Pigmentosa

The cone-specific guanylate cyclase-activating protein 3 (GCAP3), encoded by the GUCA1C gene, has been shown to regulate the enzymatic activity of membrane-bound guanylate cyclases (GCs) in bovine and teleost fish photoreceptors, to an extent comparable to that of the paralog protein GCAP1. To date, the molecular mechanisms underlying GCAP3 function remain largely unexplored. In this work, we report a thorough characterization of the biochemical and biophysical properties of human GCAP3, moreover, we identified an isolated case of retinitis pigmentosa, in which a patient carried the c.301G > C mutation in GUCA1C, resulting in the substitution of a highly conserved aspartate residue by a histidine (p.(D101H)). We found that myristoylated GCAP3 can activate GC1 with a similar Ca²⁺-dependent profile, but significantly less efficiently than GCAP1. The non-myristoylated form did not induce appreciable regulation of GC1, nor did the p.D101H variant. GCAP3 forms dimers under physiological conditions, but at odds with its paralogs, it tends to form temperature-dependent aggregates driven by hydrophobic interactions. The peculiar properties of GCAP3 were confirmed by 2 µs molecular dynamics simulations, which for the p.D101H variant highlighted a very high structural flexibility and a clear tendency to lose the binding of a Ca²⁺ ion to EF3. Overall, our data show that GCAP3 has unusual biochemical properties, which make the protein significantly different from GCAP1 and GCAP2. Moreover, the newly identified point mutation resulting in a substantially unfunctional protein could trigger retinitis pigmentosa through a currently unknown mechanism.