The relationship between fatty acid peroxidation and α-tocopherol consumption in isolated normal and transformed hepatocytes

The response of normal and transformed rat hepatocytes to oxidative stress was investigated. Isolated normal rat hepatocytes and differentiated hepatoma cells (the Fao cell line was derived from the Reuber H 35 rat hepatoma) in suspension were incubated with the ADP/Fe³⁺ chelate for 30 min at 37°C. Membrane lipid oxidation was assessed by measuring (i) free malondialdehyde (MDA) production by a high-performance liquid chromatography (HPLC) procedure, (ii) membrane fatty acid disappearance as judged by capillary gas chromatography, and (iii) α-tocopherol oxidation as determined by HPLC and electrochemical detection. The addition of iron led to increased MDA production in normal as well as in transformed cells, and to simultaneous consumption of polyunsaturated fatty acids (PUFA) and α-tocopherol. In addition, in Fao cells more α-tocopherol was consumed during lipid peroxidation while less PUFA was oxidized. Lipid peroxidation was lower in tumoral hepatocytes than in normal cells. This could be due to a difference in membrane lipid composition because of a lower PUFA content and a higher α-tocopherol level in Fao cells. During oxidation, Fao cells produced 1.5 to 2 times less MDA than normal cells, while in the tumoral cells the amount of oxidized PUFA having 3 or more double bonds was 7 to 8 times lower. Therefore, measuring MDA alone as an index of lipid peroxidation did not allow for proper comparison of the membrane lipid oxidizability of transformed cellsvs. the membrane lipid oxidizability of normal cells.     

Three-Dimensional Numerical Modeling of Mixing at River Confluences

Lateral mixing of a pollutant is considered as a slow process that is usually complete within 100–300 river widths. Recent studies on flow dynamics at river confluences revealed that lateral mixing can be markedly enhanced when the tributary channel is shallower than the main channel. This study uses a three-dimensional model to examine mixing processes immediately downstream of confluences as well as further downstream in the mainstream. Simulations are presented for a concordant and discordant laboratory junction and a field confluence for a low and a high flow condition. The decrease in standard deviation at a cross section of a tracer over a distance of 5 channel widths is 30% for discordant beds but only 10% for concordant beds in the laboratory simulation. At the natural site, bed discordance is more important at the low flow than at the high flow with corresponding decreases in the standard deviation of 31 and 18% over 3.5 channel widths. Mixing is completed after a distance of 25 and 37 channel widths for the low and high flow conditions, respectively. Further downstream, mixing is mainly affected by planform curvature of the channel.     

Soy protein isolate nanocomposite film enriched with eugenol,an antimicrobial agent: Interactions and properties

Nanocomposites films were designed from soy protein isolates (SPI), clays (Na⁺‐MMT), and eugenol an antimicrobial agent. Interactions between Na⁺‐MMT and eugenol were evidenced by a shift of the d‐spacing by X‐ray diffraction analysis. The addition of Na⁺‐MMT (5 and 7.5% w/w) in SPI solution increased its shear thinning behavior and its consistency. Accordingly, a good exfoliation of clays in SPI films was observed. The glass transition temperature of SPI films was impacted by the clays addition but not the water vapor permeability. In contrast, the addition of eugenol in SPI solution did not affected the consistency but induced a decrease of the SPI film T_g and an increase of the water vapor permeability. The presence of eugenol counterbalanced the effect of clays on consistency of film‐forming solution. The clay intercalation process was facilitated and the water vapor permeability and active agent release were modified. The presence of clay did not affect the antibacterial effect of eugenol/SPI films. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018 , 135, 45941.     

Offset printing inks based on rapeseed oil and sunflower oil. Part II: Varnish and ink formulation

Two sets of variable oil length, alkyd resins modified by sunflower oil (SOA) and by rapeseed oil (ROA), were evaluated in offset formulations with mineral oils as diluent. The more suitable alkyds for this kind of application were determined. In a second experiment, hydrocarbon solvents were substituted by the fatty acid methyl esters derived from rapeseed oil or sunflower oil to produce ecologically friendly offset printing inks. Finally, the ROA and the SOA were associated with the methyl esters derived from the same vegetable oil. New properties of the varnishes composed of a vegetable diluent were evaluated. The quickset formulations with the methyl esters do not need important modifications, as opposed to the heatset formulations.     

Pine forest investigation using high resolution P-band Pol-InSAR data

A Pol-InSAR dataset acquired at P-band over a maritime pine forest (Nezer forest of Landes, France) has been investigated and compared with tree structural ground measurements in order to identify scatterers associated with elementary scattering mechanisms in the canopy layer. Since this managed forest is constituted by different height stands, it allows to investigate mechanism change in the vertical direction during the forest growth. It has been observed that VV channel was the most elevated, which can be accounted for a maximum attenuation in the vertical direction and partially for the presence of sub-vertical branches in the top canopy layer, and that this channel was the most sensitive to the tree density in reason of an important ground reflectivity. The most pure double-bounce scattering mechanism component is received in HH-VV channel where the corresponding phase center reaches the ground level for large Diameter at Breast Height. It is also supposed that HH channel, which receives double bounce backscatter, was affected by the sub-horizontal primary branch development, which induces an elevation of the phase center over oldest trees while tree density diminution enhances backscatter from the ground level. Finally, the crosspolar channel appeared the less perturbed by the tree density than VV channel since it receives predominantly the canopy contribution.     

Apoptosis during ZIKA Virus Infection: Too Soon or Too Late?

Cell death by apoptosis is a major cellular response in the control of tissue homeostasis and as a defense mechanism in the case of cellular aggression such as an infection. Cell self-destruction is part of antiviral responses, aimed at limiting the spread of a virus. Although it may contribute to the deleterious effects in infectious pathology, apoptosis remains a key mechanism for viral clearance and the resolution of infection. The control mechanisms of cell death processes by viruses have been extensively studied. Apoptosis can be triggered by different viral determinants through different pathways as a result of virally induced cell stresses and innate immune responses. Zika virus (ZIKV) induces Zika disease in humans, which has caused severe neurological forms, birth defects, and microcephaly in newborns during the last epidemics. ZIKV also surprised by revealing an ability to persist in the genital tract and in semen, thus being sexually transmitted. Mechanisms of diverting antiviral responses such as the interferon response, the role of cytopathic effects and apoptosis in the etiology of the disease have been widely studied and debated. In this review, we examined the interplay between ZIKV infection of different cell types and apoptosis and how the virus deals with this cellular response. We illustrate a duality in the effects of ZIKV-controlled apoptosis, depending on whether it occurs too early or too late, respectively, in neuropathogenesis, or in long-term viral persistence. We further discuss a prospective role for apoptosis in ZIKV-related therapies, and the use of ZIKV as an oncolytic agent.     

Molecular Properties of Human Guanylate Cyclase-Activating Protein 3 (GCAP3) and Its Possible Association with Retinitis Pigmentosa

The cone-specific guanylate cyclase-activating protein 3 (GCAP3), encoded by the GUCA1C gene, has been shown to regulate the enzymatic activity of membrane-bound guanylate cyclases (GCs) in bovine and teleost fish photoreceptors, to an extent comparable to that of the paralog protein GCAP1. To date, the molecular mechanisms underlying GCAP3 function remain largely unexplored. In this work, we report a thorough characterization of the biochemical and biophysical properties of human GCAP3, moreover, we identified an isolated case of retinitis pigmentosa, in which a patient carried the c.301G > C mutation in GUCA1C, resulting in the substitution of a highly conserved aspartate residue by a histidine (p.(D101H)). We found that myristoylated GCAP3 can activate GC1 with a similar Ca²⁺-dependent profile, but significantly less efficiently than GCAP1. The non-myristoylated form did not induce appreciable regulation of GC1, nor did the p.D101H variant. GCAP3 forms dimers under physiological conditions, but at odds with its paralogs, it tends to form temperature-dependent aggregates driven by hydrophobic interactions. The peculiar properties of GCAP3 were confirmed by 2 µs molecular dynamics simulations, which for the p.D101H variant highlighted a very high structural flexibility and a clear tendency to lose the binding of a Ca²⁺ ion to EF3. Overall, our data show that GCAP3 has unusual biochemical properties, which make the protein significantly different from GCAP1 and GCAP2. Moreover, the newly identified point mutation resulting in a substantially unfunctional protein could trigger retinitis pigmentosa through a currently unknown mechanism.     

Self-ordered particle trains in inertial microchannel flows

Controlling the transport of particles in flowing suspensions at microscale is of interest in numerous contexts such as the development of miniaturized and point-of-care analytical devices (in bioengineering, for foodborne illnesses detection, etc.) and polymer engineering. In square microchannels, neutrally buoyant spherical particles are known to migrate across the flow streamlines and concentrate at specific equilibrium positions located at the channel centerline at low flow inertia and near the four walls along their symmetry planes at moderate Reynolds numbers. Under specific flow and geometrical conditions, the spherical particles are also found to line up in the flow direction and form evenly spaced trains. In order to statistically explore the dynamics of train formation and their dependence on the physical parameters of the suspension flow (particle-to-channel size ratio, Reynolds number and solid volume fraction), experiments have been conducted based on in situ visualizations of the flowing particles by optical microscopy. The trains form only once particles have reached their equilibrium positions (following lateral migration). The percentage of particles in trains and the interparticle distance in a train have been extracted and analyzed. The percentage of particles organized in trains increases with the particle Reynolds number up to a threshold value which depends on the concentration and then decreases for higher values. The average distance between the surfaces of consecutive particles in a train decreases as the particle Reynolds number increases and is independent of the particles size and concentration, if the concentration remains below a threshold value related to the degree of confinement of the suspension flow.     

Antimicrobial soy protein isolate‐based films: physical characterisation,active agent retention and antifungal properties against Penicillium italicum

Soy protein isolates (SPI) films were evaluated as carriers of citral and limonene, and their physical and antifungal properties were evaluated. The presence of antimicrobials in SPI films resulted in changes on colour without affecting the transparency. Films with citral added were more extensible; however, a reduction in tensile strength and elastic modulus was observed in films with limonene added. Aroma compounds addition in films induced a slight increase in water vapour properties in relation to discontinuity of network proteins evidenced by SEM. Besides, FTIR spectra evidenced a partial alteration of SPI secondary structure. Citral was less retained than limonene. The increase in limonene release with high relative humidity was explained by increase in protein chain mobility. SPI films enriched with limonene exhibited strong antifungal activity against the postharvest decay pathogen Penicillium italicum under storage conditions.     

Investigating effects of bromide ions on trihalomethanes and developing model for predicting bromodichloromethane in drinking water

Chlorination for drinking water can form brominated trihalomethanes (THMs) in the presence of bromide ions. Recent studies have reported that bromodichloromethane (BDCM) has a stronger association with stillbirths and neural tube defects than other THMs species. In this paper, the results of an experimental investigation into the factors forming THMs in the presence of bromide ions are presented. The experiments were conducted using synthetic water samples with different characteristics (e.g., pH, temperature, dissolve organic content). Different combinations of these characteristics were considered in the experimental program. The results showed that increased bromide ion concentrations led to increases in the formation of total THMs, with higher BDCM and dibromochloromethane (DBCM), and lower chloroform formation. By increasing the pH from 6 to 8.5, increased chloroform and decreased BDCM and DBCM formation were observed. Higher bromide ions to chlorine ratios increased BDCM and DBCM and decreased chloroform formation, while higher temperatures increased BDCM, DBCM and chloroform formation. In most cases, bromoform (CHBr₃) concentrations were found to be below the detection limit. Significant factors influencing BDCM formation were identified using a statistical analysis. A model for BDCM formation was estimated from 44 experiments and statistical adequacy was assessed using appropriate diagnostics, including residual plots and an R² of 0.97. The model was validated using external data from 17 water supply systems in Newfoundland, Canada. The predictive performance of the model was found to be excellent, and the resulting model could be used to predict BDCM formation in drinking water and to perform risk-cost balance analyses for best management practices.