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971.
Raman spectroscopic studies on photoreactive retinal proteins are comprehensively described, including the basic physics of Raman scattering and illustrative examples of the types of information on the structure and function of the retinal chromophore and its environment which can be obtained from the vibrational Raman spectra. In addition, practical advice and recipes are given which should enable the reader to plan and eventually perform a Raman experiment in a photolabile retinal protein. A dominant role is played by the resonance Raman (RR) experiment with visible laser excitation which selectively probes the retinal chromophore. Much discussion is devoted to bacteriorhodopsin (bR) and its photocycle as a paradigm for a light-induced reaction of a retinal protein. Various time-resolved techniques are described to study the temporal evolution of the bR chromophore by probing RR spectra of intermediate states. Vibrational Raman spectra are interpreted in terms of structure and structural changes of the chromophore. RR spectroscopic studies on halorhodopsin, sensory rhodopsin, and visual pigments are reported, as well as on modified proteins in which retinal analogues are incorporated, and on site-specific mutants. Results of ultraviolet RR experiments which selectively probe the aromatic side chains in the protein backbone are reported. In addition, a promising new technique of near-infrared Raman excitation is discussed. Finally, application of coherent anti-Stokes Raman spectroscopy (CARS) to retinal proteins is reported. 相似文献
972.
The ElectroScan environmental scanning electron microscope (ESEM) enables wet samples to be observed by eliminating air but allowing water vapour into the sample chamber. However, evaporation from, and condensation on, the sample may occur during the pumpdown sequence used to reach this state, which means that the sample may not be in its natural state when viewed if due care is not taken. In this paper, the pumping system of the ESEM is described mathematically and expressions are derived for the evaporation and condensation. This treatment is then used to calculate the optimum pumpdown sequence. The importance of using the optimized procedure is illustrated by micrographs of fat emulsions. 相似文献
973.
对经固溶处理的二种Al-Li合金(1.94wt.%Li和2.75wt.%Li)的时效过程进行了研究。结果表明:δ’的生长速率遵从Ostward粗化动力学。1.94wt.%Li合金δ’产生于时效过程中,而2.75wt.%Li合金的δ’产生于时效的前期阶段。前者的表现体积分数明显大于根据相图估算的数伍,真实体积分数则与此值接近;后者的体积分数低于根据相图估算的数值,这可能归咎于δ’固相线的位置、统计学误差及Li更易在时效过程中贫化。 相似文献
974.
We propose a new principle for a low temperature semiconductor detector of charged particles with possibly submicron lateral resolution based on point contacts formed in the 2-dimensional electron gas (2DEG) of a GaAs-AlxGa1-xAs heterostructure using split-gates. The detector operates up to liquid nitrogen temperatures. Impinging particles excite locally extra donors in the doping layer of the heterostructure. By measuring simultaneous increase in the conductances of 3 point contacts due to the impacts of charged particles the position of penetration is calculated using the Thomas-Fermi approximation for screening in 2DEG. 相似文献
975.
EPA regulations force owners of PCB (polychlorinated biphenyl) equipment to carefully review regulatory compliance and develop PCB risk management programs. The EPA is currently planning new PCB regulations that may add additional PCB management requirements. The author briefly reviews the EPA regulations, highlighting the more commonly violated requirements. Several risk management techniques are presented and discussed. A method of quantifying PCB equipment owning cost is presented 相似文献
976.
977.
V. V. Bobrovskii V. R. Shevchenko 《Journal of Engineering Physics and Thermophysics》1994,66(1):26-29
The reaction between chlorine and an alkaline solution of hydrogen peroxide is suggested for the investigation of chemothermocapillary convection [1].Vitebsk Branch of the Institute of Solid State and Semiconductors Physics, Academy of Sciences of Belarus, Vitebsk, Belarus. Translated from Inzhenerno-Fizicheskii Zhurnal, Vol. 66, No. 1, pp. 30–33, January, 1994. 相似文献
978.
R Gouider E LeGuern J Emile S Tardieu F Cabon M Samid J Weissenbach Y Agid P Bouche A Brice 《Canadian Metallurgical Quarterly》1994,44(12):2250-2252
Fistulas secondary to Crohn's disease occur in about 33% of patients. A colobronchial fistula complicating Crohn's disease is very rare, having been reported only twice previously. We present an unusual fistula secondary to Crohn's colitis that originated from the splenic flexure and crossed the diaphragm to involve the bronchial tree. 相似文献
979.
Investigates adaptive digital notch filters for the elimination of powerline noise from biomedical signals. Since the distribution of the frequency variation of the powerline noise may or may not be centered at 60 Hz. Three different adaptive digital notch filters are considered. For the first case, an adaptive FIR second-order digital notch filter is designed to track the center frequency variation. For the second case, the zeroes of an adaptive IIR second-order digital notch filter are fixed on the unit circle and the poles are adapted to find an optimum bandwidth to eliminate the noise to a pre-defined attenuation level. In the third case, both the poles and zeroes of the adaptive IIR second-order filter are adapted to track the center frequency variation within an optimum bandwidth. The adaptive process is considerably simplified by designing the notch filters by pole-zero placement on the unit circle using some suggested rules. A constrained least mean-squared algorithm is used for the adaptive process. To evaluate their performance, the three adaptive notch filters are applied to a powerline noise sample and to a noisy EEG as an illustration of a biomedical signal 相似文献
980.
Stanisic B.R. Verghese N.K. Rutenbar R.A. Carley L.R. Allstot D.J. 《Solid-State Circuits, IEEE Journal of》1994,29(3):226-238
This paper describes new techniques for the simulation and power distribution synthesis of mixed analog/digital integrated circuits considering the parasitic coupling of noise through the common substrate. By spatially discretizing a simplified form of Maxwell's equations, a three-dimensional linear mesh model of the substrate is developed. For simulation, a macromodel of the fine substrate mesh is formulated and a modified version of SPICE3 is used to simulate the electrical circuit coupled with the macromodel. For synthesis, a coarse substrate mesh, and interconnect models are used to couple linear macromodels of circuit functional blocks. Asymptotic Waveform Evaluation (AWE) is used to evaluate the electrical behavior of the network at every iteration in the synthesis process. Macromodel simulations are significantly faster than device level simulations and compare accurately to measured results. Synthesis results demonstrate the critical need to constrain substrate noise and simultaneously optimize power bus geometry and pad assignment to meet performance targets 相似文献