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161.
Site-directed mutagenesis was used to identify the veratryl alcohol binding site of lignin peroxidase. The cDNA encoding isozyme H8 was mutated at Glu146 to both an Ala and a Ser residue. The H8 polypeptide was produced by E. coli as inclusion bodies and refolded to yield active enzyme. The wild type recombinant enzyme and the mutants were purified to homogeneity and characterized by steady state kinetics. The kcat is decreased for both mutants of Glu146. The reactivity of mutants (kcat/Km) toward H2O2 were not affected. In contrast, the kcat/Km of the mutants for veratryl alcohol were decreased by at least half. The oxidation of guaiacol by these mutants were more significantly affected. These results collectively suggest that E146 plays a central role in the binding of veratryl alcohol by lignin peroxidase. 相似文献
162.
163.
RW McCallum JD Chen Z Lin BD Schirmer RD Williams RA Ross 《Canadian Metallurgical Quarterly》1998,114(3):456-461
BACKGROUND & AIMS: No effective treatment is available for patients with gastroparesis refractory to standard medical therapy. The aim of this study was to investigate the effects of gastric pacing on gastric electrical activity, gastric emptying, and symptoms in patients with gastroparesis. METHODS: Nine patients with gastroparesis participated in this study. Four pairs of cardiac pacing wires were implanted on the serosa of the stomach. The protocol consisted of two portions: a temporary inpatient study period and an outpatient study for a period of 1 month or more. RESULTS: Gastric pacing entrained the gastric slow wave in all subjects and converted tachygastria in 2 patients into regular 3-cpm slow waves. Gastric emptying was significantly improved after the outpatient treatment with gastric pacing. The gastric retention at 2 hours was reduced from 77.0% +/- 3.3% to 56.6% +/- 8.6% (P < 0.05). Symptoms of gastroparesis were substantially reduced at the end of the outpatient treatment (1.51 +/- 0.46 vs. 2.84 +/- 0.61; P < 0.04). Eight of 9 patients no longer relied on jejunostomy tube feeding, and no adverse events were noted related to the pacing unit. CONCLUSIONS: Gastric pacing seems to be able to improve symptoms of gastroparesis and to accelerate gastric emptying in patients with gastroparesis. More controlled studies are necessary to further investigate the role of gastric pacing in clinical practice. 相似文献
164.
165.
N. Balchev K. Nenkov B. Kunev J. Pirov G. Mihova G. Fuchs 《Journal of Superconductivity and Novel Magnetism》2007,20(4):333-340
Samples with nominal compositions RuSr2R1.4Ce0.6Cu2O10–δ (R = Eu, Sm) were synthesized and their superconducting (SC) and magnetic properties were compared. A coexistence of AFM
and FM ordering below the FM transition temperature was established in the two samples. It was shown that their SC properties
are affected by a spontaneous vortex phase (SVP). The first critical fields of the two samples were calculate — Hcl≈ 70 Oe for R=Eu and 60 Oe for R=Sm. It was shown that the improved superconducting properties of the Sm-containing sample
do not affect its FM behavior. A sizable magnetoresistance was observed in the two samples at T<T
c. 相似文献
166.
Diana Nanova Sebastian Beck Andreas Fuchs Tobias Glaser Christian Lennartz Wolfgang Kowalsky Annemarie Pucci Michael Kroeger 《Organic Electronics》2012,13(7):1237-1244
The degree of charge transfer in thin films of organic charge transfer (CT)-complexes, which are deposited via thermal evaporation, is examined via infrared-spectroscopy. We demonstrate a linear relationship between the shift in the excitation energy of the CN-stretching mode of CT-complexes with the acceptor 7,7,8,8-tetracyanoquinodimethane (TCNQ) and the charge transfer. The measured correlation corresponds very well with DFT calculations. For Na-TCNQ we observe a splitting in the peak of the CN-stretching mode, which can be explained by the coupling of two modes and was confirmed by the calculations. In CT-complexes with partial charge transfer the appearance of an electronic excitation is demonstrated. 相似文献
167.
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169.
This paper presents a new method in TLM for very efficient computation of highly conducting thin shielding walls. Firstly, the impulse response of a finite conducting sheet is computed analytically via inverse Laplace transform. Subsequently, a recursive convolution integral is formulated with the analytical impulse response. This allows a very efficient and accurate calculation of the diffusion process through imperfectly conducting walls. 相似文献
170.
JK Collins BH Arjmandi PL Claypool P Perkins-Veazie RA Baker BA Clevidence 《Nutrition journal》2004,3(1):1-7