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81.
Amino acid changes S180A (S-->A at site 180), H197Y, Y277F, T285A, and A308S are known to shift the maximum wavelength of absorption (lambda max) of red and green visual pigments toward blue, essentially in an additive fashion. To test the generality of this "five-sites" rule, we have determined the partial amino acid sequences of red and green pigments from five mammalian orders (Artiodactyla, Carnivora, Lagomorpha, Perissodactyla, and Rodentia). The result suggests that cat (Felis catus), dog (Canis familiaris), and goat (Capra hircus) pigments all with AHYTA at the five critical sites have lambda max values of approximately 530 nm, whereas rat (Rattus norvegicus) pigment with AYYTS has a lambda max value of approximately 510 nm, which is accurately predicted by the five-sites rule. However, the observed lambda max values of the orthologous pigments of European rabbit (Oryctolagus cuniculus), white-tailed deer (Odocoileus virginianus), gray squirrel (Sciurus carolinensis), and guinea pig (Cavia procellus) are consistently more than 10 nm higher than the predicted values, suggesting the existence of additional molecular mechanisms for red and green color vision. The inferred amino acid sequences of ancestral organisms suggest that the extant mammalian red and green pigments appear to have evolved from a single ancestral green-red hybrid pigment by directed amino acid substitutions. 相似文献
82.
PS Estes J Roos A van der Bliek RB Kelly KS Krishnan M Ramaswami 《Canadian Metallurgical Quarterly》1996,16(17):5443-5456
Presynaptic terminals contain several specialized compartments, which have been described by electron microscopy. We show in an identified Drosophila neuromuscular synapse that several of these compartments-synaptic vesicle clusters, presynaptic plasma membrane, presynaptic cytosol, and axonal cytoskeleton-labeled by specific reagents may be resolved from one another by laser scanning confocal microscopy. Using a panel of compartment-specific markers and Drosophila shibire(ts1) mutants to trap an intermediate stage in synaptic vesicle recycling, we have examined the localization and redistribution of dynamin within single synaptic varicosities at the larval neuromuscular junction. Our results suggest that dynamin is not a freely diffusible molecule in resting nerve terminals; rather, it appears localized to synaptic sites by association with yet uncharacterized presynaptic components. In shi(ts1) nerve terminals depleted of synaptic vesicles, dynamin is quantitatively redistributed to the plasma membrane. It is not, however, distributed uniformly over presynaptic plasmalemma; instead, fluorescence images show "hot spots" of dynamin on the plasma membrane of vesicle-depleted nerve terminals. We suggest that these dynamin-rich domains may mark the active zones for synaptic vesicle endocytosis first described at the frog neuromuscular junction. 相似文献
83.
AM Ballangrud PJ Wilson K Brown GG Miller RB Moore MS McPhee J Tulip 《Canadian Metallurgical Quarterly》1996,19(4):471-479
OBJECTIVE: To compare the prevalence of conduct problems (CP) according to level of urbanization and to determine which factors account for the potential difference in prevalence rates. METHOD: Study 1 used a questionnaire survey of a nationally representative sample of 10,462 Norwegian adolescents. Study 2 used a questionnaire survey of a representative sample of 1,346 adolescents living in Oslo. Self-reported CP included most DSM-III-R criteria for conduct disorder. RESULTS: CP rates were similar in all levels of urbanization, except for the only semimetropolitan city in the country, the capital Oslo, which had CP rates twice those of the rest of the country. This increase rate could not be explained by a series of commonly advocated explanations: family structure and parental practices, social network, socioeconomic status, integration in community activities, religious involvement, and race. However, involvement in "soft" drugs and associating with antisocial peers could explain the statistically differential rates. Furthermore, in the Oslo study, adolescents' CP did not vary according to density of population or region within the city. CONCLUSIONS: The results support previous studies showing increased rates of CP in urban areas. However, urbanization must pass a certain threshold before it has this effect. Moreover, the lack of support for commonly advocated explanations for the difference between urban and nonurban areas suggests that investigations specifically addressing potential explanations for this difference should be conducted. The results indicate that the increased rates of substance use in highly urbanized areas may account for the difference in CP rates by prolonging and aggravating CP. 相似文献
84.
RB Brown 《Canadian Metallurgical Quarterly》1996,49(3):230-251
This paper examines popular concepts and "types" of organizational commitment in light of the definition of commitment and common factors that pertain to all commitments. It argues that a commitment is best conceptualized as a single, fundamental construct that may vary according to differences in focus, terms, and time-specific evaluation. Analysis of affective commitment measures indicates that certain measures contain more than one set of terms and make assumptions about evaluation that add constraints to the basic meaning of commitment. Recommendations include the argument that commitment measures address one set of terms and avoid evaluative phraseology. 相似文献
85.
Borodin, Nielsen and Rackoff [13] introduced the class of priority algorithms as a framework for modeling deterministic greedy-like algorithms. In this paper we address the effect of randomization in greedy-like algorithms. More specifically, we consider approximation ratios within the context of randomized priority algorithms. As case studies, we prove inapproximation results for two well-studied optimization problems, namely facility location and makespan scheduling. 相似文献
86.
Jens Clausen Allan Larsen Jesper Larsen Natalia J. Rezanova 《Computers & Operations Research》2010,37(5):809-821
This paper provides a thorough review of the current state-of-the-art within airline disruption management of resources, including aircraft, crew, passenger and integrated recovery. An overview of model formulations of the aircraft and crew scheduling problems is presented in order to emphasize similarities between solution approaches applied to the planning and recovery problems. A brief overview of research within schedule robustness in airline scheduling is included in the review, since this proactive measure is a natural complement to disruption management. 相似文献
87.
Allan James Samantha Low Choy Kerrie Mengersen 《Environmental Modelling & Software》2010,25(1):129-145
Expert elicitation is the process of retrieving and quantifying expert knowledge in a particular domain. Such information is of particular value when the empirical data is expensive, limited or unreliable. This paper describes a new software tool, called Elicitator, which assists in quantifying expert knowledge in a form suitable for use as a prior model in Bayesian regression. Potential environmental domains for applying this elicitation tool include habitat modelling, assessing detectability or eradication, ecological condition assessments, risk analysis and quantifying inputs to complex models of ecological processes. The tool has been developed to be user-friendly, extensible and facilitate consistent and repeatable elicitation of expert knowledge across these various domains. We demonstrate its application to elicitation for logistic regression in a geographically based ecological context. The underlying statistical methodology is also novel, utilizing an indirect elicitation approach to target expert knowledge on a case-by-case basis. For several elicitation sites (or cases), experts are asked simply to quantify their estimated ecological response (e.g. probability of presence), and its range of plausible values, after inspecting (habitat) covariates via GIS. 相似文献
88.
Laxman U. S. Nayak Lee Priest Allan P. White 《Universal Access in the Information Society》2010,9(4):367-374
In this cross-sectional study, the principles of a technology acceptance model were used to identify variables related to
the level of Internet usage by older adults. Community-dwelling older adults aged 60–88 years completed a postal questionnaire
survey that elicited responses on the use of the Internet. Out of a sample of 592 older adults (236 males and 356 females),
50.7% used the Internet. A multiple linear regression analysis was carried out on the Internet users sample using the self-reported
number of hours of Internet usage per week as the dependent variable. The results indicated that attitude toward using the
Internet and good health status were statistically significant predictors of the level of Internet usage. A second multiple
regression analysis using Internet activity as the dependent variable showed that attitude, usefulness, good health, and gender
(males) were significant predictor variables. 相似文献
89.
Benchmarking Image Segmentation Algorithms 总被引:1,自引:0,他引:1
We present a thorough quantitative evaluation of four image segmentation algorithms on images from the Berkeley Segmentation
Database. The algorithms are evaluated using an efficient algorithm for computing precision and recall with regard to human
ground-truth boundaries. We test each segmentation method over a representative set of input parameters, and present tuning
curves that fully characterize algorithm performance over the complete image database. We complement the evaluation on the
BSD with segmentation results on synthetic images. The results reported here provide a useful benchmark for current and future
research efforts in image segmentation. 相似文献
90.
Milosavljevic S Gregory DE Pal P Carman AB Milburn PD Callaghan JP 《Applied ergonomics》2011,42(6):801-806
Wool handling is an important rural occupation where workers process 200 or more fleeces daily, separating them into various quality components. Loads and postures they experience carry substantial risk of low back pain (LBP). Although a formal skill training structure exists, interaction with loads and LBP is unknown. We examined whether skill and LBP influenced trunk postures and loads of 60 wool handlers representing 3 skill levels. LBP prevalence ranged from 20% for junior (lowest skill) to 45% for open class (highest skill) wool handlers. Open class wool handlers demonstrated increased lateral bend and more axially twisted postures, generating greater medio-lateral shear forces and lateral bend and axial twist moments. LBP was associated with open class wool handlers spending more time in severe axially twisted postures. These findings suggest that skill-based training needs to be reviewed to reduce the quantity of axially twisted posture which may help reduce the prevalence of LBP in this workforce. 相似文献