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121.
The cytochemical properties of metaphase chromosomes from Chinese hamster and human cells were studied by flow cytometry. This technique allows precise quantitation of the fluorescence properties of individual stained chromosome types. Chromosomes were stained with the following fluorescent DNA stains: Hoechst 33258, DAPI, chromomycin A3, ethidium bromide, and propidium iodide. The relative fluorescence of individual chromosome types varied depending on the stain used, demonstrating that individual chromosome types differ in chemical properties. Flow measurements were performed as a function of stain and chromosome concentration to characterize the number and distribution of stain binding sites. Flow analysis of double stained chromosomes show that bound stains interact by energy transfer with little or no binding competition. For most hamster chromosomes, there is a strong correlation between relative fluorescence and stain base preference suggesting that staining differences may be determined primarily by differences in average base composition. A few hamster chromosome types exhibit anomalous staining which suggests that some other property, such as repetitive DNA sequences, also may be an important determinant of chromosomal staining. 相似文献
122.
A review of 227 cases of invasive carcinoma of the cervix was undertaken to determine the efficacy of procedures using in the staging of this disease. All patients had a pretreatment chest radiography and intravenous pyelogram. 96.5% had pretreatment cystoscopy, 98.6% had pretreatment proctoscopy, and 92% had a pretreatment barium enema. These patients were retrospectively staged on the findings of physical examination only. Each additional procedure was then evaluated by comparison with the initial staging. Cystoscopy and chest film findings each would have changed the clinical stage in fewer than 1% of cases, barium enema in 1.4%, and proctoscopy in 2.2%. No patient had a positive barium enema without a positive proctoscopy, while two patients had positive proctoscopies with negative barium enemas. The overall yield of positive findings by pretreatment intravenous pyelography was 7.3%. Lymphangiography, although inconsistently done, yielded positive findings in all stages. Chest radiographs, intravenous pyelography, proctoscopy, and lymphangiography are recommended as part of the pretreatment workup of patients with carcinoma of the cervix. Routine barium enema is no longer recommended, and cystoscopy is only recommended in patients with clinical stage IIB disease or greater. 相似文献
123.
GENOMIC HARDWIRING AND PHENOTYPIC PLASTICITY OF TERPENOID-BASED DEFENSES IN CONIFERS 总被引:8,自引:0,他引:8
Over evolutionary history, conifers have faced a myriad of threats from phloem- and xylem-feeding insects, defoliating insects, and fungal pathogens. Among the trees defenses, terpenoids appear to play a major role by harming, disabling, deterring, repelling, or otherwise reducing the fitness of potential invaders. Each of the three classes of terpenoids in conifers, monoterpenes, sesquiterpenes, and diterpenes, are composed of a large number of representative compounds. In most cases, the presence of a particular terpenoid compound in the oleoresin or volatile emissions from a specific conifer can be accounted for by the expression of one of many committed terpene synthase (TPS) genes. However, while each TPS may produce one or a few major products, many produce a variety of minor products with relatively constant component ratios in the product blends. TPS genes exist in conifers in large and functionally diverse, yet monophyletic, gene families. Within these gene families, new biochemical functions of TPS appear to have evolved by gene duplication and changes in the amino acid sequence of the enzymes active site. In addition, TPS genes may be differentially expressed prior to, during, and following attack by insects or pathogens. Thus, while the production of any particular terpenoid is hardwired into a conifers genome, these trees have the capacity to change the mixture of terpenoids in oleoresin secretions and volatile emissions. Anatomical changes may also accompany induced terpenoid production, supplementing the plasticity of the molecular and biochemical events. 相似文献
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TJ Hall M Cooper RG Hughes B Levin DB Skinner AR Moossa 《Canadian Metallurgical Quarterly》1977,134(5):544-548
The clinical, surgical, and pathologic findings in a five year prospective study of 192 patients referred with a high probability of pancreatic cancer are reported. We have defined the requirements of any pancreatic imaging procedure as its ability to distinguish a normal pancreas from pancreatic cancer or chronic pancreatitis and the capability of detecting tumors less than 5 cm in diameter. There was a 47 percent incidence of pancreatic disease (27 percent pancreatic cancer and 20 percent chronic pancreatitis). Prospective radionuclide imaging as routinely performed was found to be of little clinical value in this patient population; it was neither specific nor sensitive to pancreatic cancer or chronic pancreatitis. Preliminary data with longitudinal multiplane emission tomography show an improved diagnostic accuracy and the ability to detect resectable tumors, but its efficacy needs to be prospectively compared with other screening tests on a carefully defined patient population. 相似文献
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The paper (a) assesses the social and economic demands for water-related recreational, leisure and amenity facilities, (b) reviews the ways in which these demands are currently satisfied for lakes, reservoirs, rivers and canals, and (c) identifies possible future trends. The significant degree of interaction with other uses of water is also discussed. 相似文献
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J. Hensman R. Mills S.G. Pierce K. Worden M. Eaton 《Mechanical Systems and Signal Processing》2010,24(1):211-223
A standard technique in the field of non-destructive evaluation is to use acoustic emissions to characterise and locate the damage events that generate them. The location problem is typically posed in terms of the times of flight of the waves and results in an optimisation problem, which can at times be ill-posed. A method is proposed here for learning the relationship between time of flight differences and damage location using data generated by artificially stimulated acoustic emission (AE)—a classic problem of regression. A structure designed to represent a complicated aerospace component was interrogated using a laser to thermoelastically generate AE at multiple points across the structure's surface. Piezoelectric transducers were mounted on the surface of the structure, and the resulting waveforms were recorded. A Gaussian process (GP) with RBF kernels was chosen for regression. Since during AE monitoring not all events can be guaranteed to be detected by all sensors, a GP was trained on data for all possible combinations (subsets) of sensors. The inputs to the GPs were the differences in time of flight between sensors in the set, and the targets were the locations of the source of ultrasonic stimulation. Subsequent (test) data points were located by every possible GP, given the active sensors. It is shown that maps learned on a given structure can generalise effectively to nominally identical structures. 相似文献