The Use of Flash Chromatography for the Isolation and Purification of Glucosinolates (Mustard Oil Glycosides)

A rapid method for the isolation of several important glucosinolates in large (gram) amounts using flash chromatography is described. Emphasis has been paid to the major glucosinolates of the meal and green material of rape, Brassica napus, to enable further studies on their bioactivity and to supply standards for analytical purposes. The application of the method for the isolation of other glucosinolates is discussed.     

Levels of the herbicide glyphosate in well water

The prognostic significance of tumour cell proliferation was investigated in a series of 418 gastric carcinomas using the monoclonal antibody MIB-1. Owing to strong intratumoural heterogeneity of MIB-1 expression three different proliferation indices (PIs) were determined in all carcinomas: (1) PImax in areas of maximal tumour cell proliferation, (2) PIrand in areas randomly distributed over the whole tumour. (3) PIfront in areas exclusively located at the tumour invasion front. There was a strong intertumoral heterogeneity with PImax ranging from 4.9% to 92.2%, PIrand ranging from 3.4% to 81.4% and PIfront ranging from 4.2% to 87.1%. The mean values were 51.3% +/- 19.7 for PImax, 34.2% +/- 18.3 for PIrand and 37.2% +/- 19.5 for PIfront. Whereas no statistically significant correlation could be found between proliferative activity and the clinicopathological parameters depth of invasion, lymph node involvement or grade of tumour differentiation, there was a positive correlation between a high proliferation index at the tumour invasion front (PIfront) and the presence of blood or lymphatic vessel invasion. No significant correlation could be demonstrated between the different proliferation indices and survival, even when different subgroups of patients were analysed separately. The present results suggest that the immunohistochemical evaluation of the proliferation activity has no predictive value for the prognosis of gastric cancer patients or the identification of subgroups of patients who may be at higher risk.     

Euthanasia: going Dutch?

From 1970 to 1990 the authors treated 4 patients with deep colitis (DCC). There were 2 males and 2 females aged from 19 to 48 years (average age 28.7 years). A local form of DCC with involvement of the rectum (infero- and preampullar part) was recognized in all cases. The only method for DCC treatment is an operative intervention whose volume is determined by the form of the disease and the results of emergency intraoperative morphological study of the affected parts of the large intestine. All 4 patients underwent organ- and sphincter-preserving operation-transanal resection of the rectum. The postoperative period was uneventful. None of the patients had a recurrence of the disease in follow-up periods of 3 to 7 years.     

Clinical comparison of resorbable and non-resorbable barriers for guided periodontal tissue regeneration

The purpose of this study was to compare the clinical results of guided periodontal tissue regeneration (GPTR) using a resorbable barrier manufactured from a copolymer of polylactic and polyglycolic acids (Resolut Regenerative Material) with those of non-resorbable e-PTFE barrier (Gore-Tex Periodontal Material). 12 subjects participated, 6 with similarly paired class II furcations and 6 with 2 similar 2, 3-wall periodontal lesions. The resorbable and non-resorbable barriers were randomly assigned to 1 defect in each subject. Non-resorbable barriers were removed in six weeks. Plaque index (PlI), gingival index (GI), probing depth (PD), clinical attachment level (CAL) and gingival recession (R) were recorded at baseline, (i.e., immediately prior to surgery) and at 12 months postsurgically. The clinical healing was similar and uneventful in both groups. Intrabony pockets depicted significant changes from baseline (p < 0.05) for probing depth reduction and gain in clinical attachment levels. No differences were found between treatments. Class II furcations showed significant improvements from baseline (p < or = 0.05) for probing depth reduction and clinical attachment gain. No differences were detected between treatments. It is concluded that the resorbable barrier tested is as effective as the nonresorbable e-PTFE barrier for the treatment of class II furcations and intrabony defects.