全文获取类型
收费全文 | 5610篇 |
免费 | 1篇 |
国内免费 | 1篇 |
专业分类
化学工业 | 37篇 |
机械仪表 | 8篇 |
建筑科学 | 5篇 |
能源动力 | 5篇 |
轻工业 | 21篇 |
水利工程 | 4篇 |
无线电 | 42篇 |
一般工业技术 | 43篇 |
冶金工业 | 5434篇 |
原子能技术 | 1篇 |
自动化技术 | 12篇 |
出版年
2023年 | 2篇 |
2021年 | 4篇 |
2020年 | 1篇 |
2019年 | 1篇 |
2018年 | 1篇 |
2014年 | 3篇 |
2013年 | 12篇 |
2012年 | 6篇 |
2011年 | 7篇 |
2010年 | 1篇 |
2009年 | 1篇 |
2008年 | 2篇 |
2007年 | 5篇 |
2006年 | 11篇 |
2005年 | 5篇 |
2004年 | 8篇 |
2003年 | 15篇 |
2002年 | 5篇 |
2001年 | 3篇 |
2000年 | 7篇 |
1999年 | 165篇 |
1998年 | 1661篇 |
1997年 | 904篇 |
1996年 | 642篇 |
1995年 | 328篇 |
1994年 | 314篇 |
1993年 | 333篇 |
1992年 | 39篇 |
1991年 | 58篇 |
1990年 | 86篇 |
1989年 | 67篇 |
1988年 | 67篇 |
1987年 | 85篇 |
1986年 | 63篇 |
1985年 | 58篇 |
1984年 | 3篇 |
1983年 | 13篇 |
1982年 | 34篇 |
1981年 | 35篇 |
1980年 | 40篇 |
1979年 | 1篇 |
1978年 | 10篇 |
1977年 | 143篇 |
1976年 | 337篇 |
1975年 | 13篇 |
1974年 | 1篇 |
1971年 | 2篇 |
1968年 | 2篇 |
1965年 | 1篇 |
1955年 | 5篇 |
排序方式: 共有5612条查询结果,搜索用时 296 毫秒
71.
RL Prestidge PM Grandison DW Chuk RJ Booth JD Watson 《Canadian Metallurgical Quarterly》1995,164(1):129-132
The 19-kDa antigen (19Ag) of Mycobacterium tuberculosis (Mt) is a lipoprotein which is released from the organism during growth. In order to study the possible involvement of this antigen in the host protective response against Mt infection, it would be helpful to obtain high-level production of 19Ag from a recombinant organism. We have found that overexpression of the native 19Ag gene in Escherichia coli or yeast leads to products which are aggregated and insoluble. By site-directed mutagenesis of the 19Ag lipoprotein leader sequence, we have generated a mutant gene which directs the production of 19Ag into the periplasmic space of E. coli, from where it can be easily purified in high yield. 19Ag obtained from this mutant construct lacks the lipid-modified N-terminal Cys residue found in the native 19Ag, and is not glycosylated, but is otherwise indistinguishable from 19Ag isolated from Mt culture supernatant. 相似文献
72.
73.
74.
75.
76.
SA Syed KS Zahir JR Zink O Saglaam RJ Restifo JG Thomson 《Canadian Metallurgical Quarterly》1997,38(4):396-403
Reinstatement and spontaneous recovery of previously extinguished nicotine-taking behavior were examined in rats. Male subjects were trained to self-administer nicotine (30 microg/kg per infusion, IV; one 60-min session per day for 3 weeks). Extinction sessions were then given for 5-10 days during which saline was substituted for nicotine. Subsequently, in the first set of tests for nicotine seeking, the reinstatement of lever presses that previously delivered nicotine was examined after priming injections of saline and nicotine (75, 150 and 300 microg/kg, SC; and 30 and 60 microg/kg, IV). In the second set of tests for nicotine-seeking, rats were tested after an additional 21-day drug-free period during which they were not exposed to the self-administration chambers (a test for the spontaneous recovery of drug seeking), and after priming injections of nicotine (150 and 300 microg/kg, SC). Reinstatement of extinguished food-reinforced behavior after exposure to nicotine was also determined. Priming injections of nicotine reinstated nicotine seeking regardless of the route of administration. In addition, previously extinguished nicotine seeking recovered spontaneously after a 21-day period during which rats were not exposed to the drug-taking environment. Nicotine also reinstated extinguished food-reinforced behavior in rats with a history of nicotine self-administration, but not in drug-naive rats. The present results extend previous work with opioid and stimulant drugs on reinstatement of drug seeking by the self-administered drug. It also appears that, as with other positive reinforcers, the mere passage of time is a sufficient condition for the spontaneous recovery of extinguished nicotine seeking. 相似文献
77.
Coronary angiography in a 52-year-old woman with angina-type chest pain showed tumor circulation with feeding arteries arising from the circumflex artery. Transthoracic echocardiography and magnetic resonance imaging both failed to show any intracardiac masses. A sessile mass measuring 1.5 cm in diameter attached to the atrial septum was readily detected by transesophageal echocardiography. Histologic analysis confirmed the tumor to be a myxoma. Coronary angiography may provide the first clue to the presence of a small myxoma. Transesophageal echocardiography is the imaging modality of choice for further evaluation. 相似文献
78.
P Dhordain O Albagli RJ Lin S Ansieau S Quief A Leutz JP Kerckaert RM Evans D Leprince 《Canadian Metallurgical Quarterly》1997,94(20):10762-10767
79.
Damage to cochlear efferents in chinchillas was assessed using transmission electron microscopy following unilateral treatment with the cholinotoxin ethylcholine mustard aziridinium ion (AF64A). AF64A was diluted in artificial perilymph to concentrations ranging from 0.5 to 100 microM. Survival times ranged from 1 to 12 weeks. At concentrations above 10 microM, widespread damage was noted to efferent fibers within the inner spiral bundle (ISB), tunnel spiral bundle (TSB), tunnel radial fibers (TRF) and efferent terminals at the base of OHCs. This damage included degeneration of fibers and terminals, delamination of mitochondria, vacuolization, and loss of cell membrane. However, at high concentrations, non-specific damage was also noted as thinnings or discontinuities of the membrane of OHCs and afferent fibers. At concentrations between 3 and 10 microM, selective damage was observed to efferent fibers within the ISB, TSB, TRF, and to terminals at the base of the OHCs, with all other structures appearing normal. At concentrations of 0.5 and 1 microM, damage was limited to efferent fibers within the TSB and ISB below the inner hair cells. In general, insult was greatest to middle- and basal-turn efferents, and longer survival times did not produce greater damage to, or loss of, efferents. These data suggest that at low concentrations, AF64A produces a partial yet selective degeneration of cochlear efferents within both the medial and lateral tracts, and that at the lowest concentrations used in these studies, AF64A produces a preferential insult on lateral olivocochlear efferents. 相似文献
80.
Abnormal hepatic mitochondrial respiration and cytochrome C oxidase activity in rats with long-term copper overload 总被引:2,自引:0,他引:2
RJ Sokol MW Devereaux K O'Brien RA Khandwala JP Loehr 《Canadian Metallurgical Quarterly》1993,105(1):178-187
BACKGROUND: Dietary copper overload in the rat is associated with morphological abnormalities and lipid peroxidation of hepatic mitochondria. This study was designed to determine if copper hepatotoxicity was associated with functional alterations in mitochondrial respiration in conjunction with lipid peroxidation. METHODS: Weanling male rats were pair-fed for 8 weeks on diets containing normal or high levels of copper in combination with sufficient vitamin E. Serum and liver samples were obtained, and hepatic mitochondria were isolated by differential centrifugation. RESULTS: Oxidant injury (decreased levels of hepatic glutathione and alpha tocopherol and increased levels of mitochondrial thiobarbituric acid-reacting substances) was present in the copper-overloaded rats. Serum aminotransferase levels correlated with concentrations of mitochondrial copper and thiobarbituric acid-reacting substances. Copper overload caused a decrease in state 3 respiration and the respiratory control ratio in hepatic mitochondria when several electron donors were used. Analysis of the oxidoreductase activities of the four mitochondrial electron transport protein complexes showed that complex IV (cytochrome C oxidase) activity was reduced by 60% in copper overload. CONCLUSIONS: Functional abnormalities of mitochondria accompany lipid peroxidation and the morphological alterations caused by copper overload, supporting the hypothesis that the mitochondrion is one of the major intracellular targets in copper hepatotoxicity. 相似文献