Report from the Wellcome Trust/EBA “Perspectives in Clinical Proteomics” retreat--a strategy to implement next-generation proteomic analyses to the clinic for patient benefit: pathway to translation

Proteomics has long been thought to hold the promise of producing results of clinical utility which will influence patient treatment and outcomes. A recent Wellcome Trust/EBI meeting and retreat--“Perspectives in Clinical Proteomics”--brought together experts from a broad range of stakeholder groups with an interest in ensuring proteomics achieves this aim. This viewpoint presents views derived from these forums, proposing a pathway for the development of next-generation proteomic analyses in the clinical setting from selection of candidates through to their validation and ultimate demonstration of utility through health technology assessments. Although not meant to be all encompassing, important elements for proteomics researchers to consider are presented.     

A cross-junction channel valveless-micropump with PZT actuation

A gas-jet micro pump with novel cross-junction channel has been designed and fabricated using a Si micromachining process. The valveless micro pump is composed of a piezoelectric lead zirconate titanate (PZT) diaphragm actuator and fluidic network. The design of the valveless pump focuses on a cross-junction formed by the neck of the pump chamber and one outlet and two opposite inlet channels. The structure of cross-junction allows differences in fluidic resistance and fluidic momentum inside the channels during each PZT diaphragm vibration cycle, which leads to the gas flow being rectified without valves. The flow channels were easily fabricated by using silicon etching process. To investigate the effects of the structure of the cross-junction on the gas flow rate, two types of pump with different cross-junction were studied. The design and simulation were done using ANSYS-Fluent software. The simulations and experimental data revealed that the step-nozzle structure is much more advantageous than the planar structure. A flow rate of 5.2 ml/min was obtained for the pump with step structure when the pump was driven at its resonant frequency of 7.9 kHz by a sinusoidal voltage of 50 V_p–p.     

Creating broadcast interactive drama in an NVE

By exploiting a networked virtual environment architecture, we produced the gaming part of an interactive television drama series, TypoToons. For decades TV show makers have been inventing tools and tricks to enhance the stories they tell. Equivalents of, and additions to these conventional tricks in interactive media can only mature an interactive television if multidisciplinary teams can obtain hands on experience of these newly emerging worlds. The production team for TypoToons and the interactively participating audience provided an excellent opportunity to develop such a team     

The use of hypertext in the writing of group papers

Abstract The Virtual Constructing Tool (VCT) was developed at the Nijmegen School of Management in 1998. The VCT is intended to facilitate the process of writing group papers through the use of construction and communication tools and is based completely on the Internet. The VCT allows a paper to be structured by means of hyperlinking and cooperation between students. Students are allowed to divide tasks, but they are held responsible for the quality of the arguments in the paper as a whole. They are invited to spend time in dealing with validity claims and in building a valid train of thought. Producing a group paper includes four interrelated working processes: exploring the subject, exchanging information, arguing and discussing and editing. Hyperlinking offers a contribution in each of these processes which varies from associative linking of pieces of information to strengthening linear structuring. Therefore, a different perspective has been taken to that of those authors who consider hyperlinking as a tool par excellence for the nonlinear production of information.     

Identification of stable models in subspace identification by usingregularization

In subspace identification methods, the system matrices are usually estimated by least squares, based on estimated Kalman filter state sequences and the observed inputs and outputs. For a finite number of data points, the estimated system matrix is not guaranteed to be stable, even when the true linear system is known to be stable. In this paper, stability is imposed by using regularization. The regularization term used here is the trace of a matrix which involves the dynamical system matrix and a positive (semi) definite weighting matrix. The amount of regularization can be determined from a generalized eigenvalue problem. The data augmentation method of Chui and Maciejowski (1996) is obtained by using specific choices for the weighting matrix in the regularization term     

Three-dimensional simulation of time-coded emission tomography

In this paper we discuss the potentialities of a time-coded single photon emissive imaging system for thyroid tomography. We have performed three-dimensional simulation studies in order to answer two questions: 1) does this coded aperture device produce good quality reconstructions, and 2) can the reconstruction be carried out sufficiently fast on a microcomputer. Our study leads to the conclusion that both questions can be answered affirmatively. Hence, time-coded emission tomography is a potentially useful imaging technique for diagnostic clinical practice.     

A family of similarity measures between two strings

We present a class of similarity measures for quantitatively comparing two strings, that is, two linearly ordered sets of elements. The strings can be of different lengths, the elements come from a single alphabet, and an element may appear any number of times. The limiting values of each measure are 0, when two completely different strings are compared, and 1, when the two strings are identical. Applications of similarity measures are numerous in nonnumerical computations, such as in heuristic search processes in associative networks, in pattern recognition and classification, in game playing programs, and in music and text analysis. We offer a number of feasible measures from among which some are discarded on plausibility grounds. One can select the measure most adequate for one's needs on the basis of a few characteristic examples of strings compared and by considering the specific requirements of the application at hand.