Temperature monitoring

Central temperature is usually tightly regulated in human beings. Anesthesia alters the normal thermoregulatory controls of the body. Intraoperatively, mild degrees of hypothermia may provide some cerebral protection. However, the risk of organ dysfunction and shivering require that the anesthesiologist be prepared to treat severe hypothermia. Appropriate measures such as warning the operating room and using forced air blankets can prevent both intraoperative hypothermia and postoperative shivering. The use of temperature measurement is not limited to the operative and immediate recovery periods. Anesthesiologists practicing in intensive care units and in pain clinics use temperature monitoring as a diagnostic tool in a variety of situations.     

GaInP/GaAs HBTs for high-speed integrated circuit applications

The use of GaInP/GaAs heterojunction bipolar transistors (HBTs) for integrated circuit applications is demonstrated. The discrete devices fabricated showed excellent DC characteristics with low V_ce offset voltage and very low temperature sensitivity of the current gain. For a non-self-aligned device with a 3-μm×1.4-μm emitter area, f_T was extrapolated to 45 GHz and f_max was extrapolated to 70 GHz. The measured 1/f noise level was 20 dB better than that of AlGaAs HBTs and comparable to that of low-noise silicon bipolar junction transistors, and the noise bump (Lorentzian component) was not observed. The fabricated gain block circuits showed 8.5 dB gain with a 3-dB bandwidth of 12 GHz, and static frequency dividers (divide by 4) were operable up to 8 GHz     

Cold forming processes: some examples of predictions and design optimization using numerical simulations

Predicting the behaviour of steel during a deformation process, and then under service conditions, is one of the main challenges in cold forming. The design of optimized forging schedules, by means of classical trial+errors procedures, has become increasingly heavy in terms of time and cost in a competitive environment. Simultaneously, the improvement of steel qualities requires the microstructure, constitutive behaviour and deformability to be known a priori regarding a targeted application. During the last few years, numerical simulations have become a very efficient tool to reach these goals.

In this paper, we give examples of innovating forging sequences developed by numerical simulations, including the investigation of damage in tools and forged parts. In case of specific processes with very determined geometry — such as wire drawing — we show how systematic numerical studies may lead to predictive models of force, local strains and residual stress…

However, reliable predictions from numerical simulations require reliable input data, including constitutive laws, friction conditions and propensity to ductile damage. These data must be characterized under realistic sollicitations. Typical cold forging loadings are indeed very severe: local strains up to 600%, strain rates locally greater than 1000 s⁻¹, and subsequently, plastic heating over 500°C.

To characterize the constitutive behaviour, the standard upset test between grooved dies is used along with an original methodology to derive the strain hardening curve from the experimental force-displacement recording. Tool elastic deformations, specimen strain heterogeneity… are taken into account. This enables a precise determination of the strain hardening curve up to about 100% of strain. The extrapolation of the flow stress to greater deformations is then very easy and reliable. Such a test can be performed under quasi-static and isothermal conditions (0.1 s⁻¹ but also adiabatic and rapid conditions (up to 10 s⁻¹). This procedure was adapted to a Pellini hammer, which enables very simple characterization at 800 s⁻¹. The comparison of all these flow curves lead to the formulation of an original constitutive model, which accounts for the effects of plastic heating, strain rate, dynamic aging…

In order to predict ductile fracture during the forging process, the most classical criteria were tested over a wide range of experimental loading conditions. None of them were general enough to solve all the cold forming problems. On the other hand, mesoscopic models describing the deformation of the metal matrix around inclusions or second phases have proved to be in good agreement with the various experimental observations. An original plasticity criterium, based on the recent works in porous plasticity theory, has been developed and already displays promising capabilities. Simple experimental procedures enable a reliable classification of steel qualities, heat treatments… in term of forgeability.

Finally, the friction problem is treated using different methodologies based on the forming process considered. For forging operations, a fine analysis of the force-displacement curves in direct extrusion stages may lead to a precise measurement of the friction coefficient under pressures from about 200 MPa up to 1000 MPa and for sliding rates between 1 and 100 mm/s. For wire drawing process, a model relying on an analytical approach using a significantly improved slice method has been developed: the comparison of the experimental drawing force and the predicted one gives the friction coefficient in industrial processing conditions (speed up to 5 m/s).     

Relationship between the attenuation of downwelling irradiance at 490 nm with the attenuation of PAR (400 nm-700 nm) in the Baltic Sea

The vertical attenuation coefficient of diffuse downwelling irradiance at 490 nm (K_d 490) is a parameter that we routinely derive from SeaWiFS images of the Baltic Sea. Here, through model simulations, we examine the relationship between K_d(490), and the vertical attenuation coefficient of PAR (K_d PAR), as this later coefficient determines the light available for aquatic photosynthesis. A simple semi-analytical model is used to predict K_d(490) and K_d(PAR), as a function of the concentrations of chlorophyll, colored dissolved organic material (CDOM), suspended inorganic, and suspended organic particulate material. A series of model simulations based on variations in these optically significant constituents over a range realistic for the Baltic Sea, are used to define the relationship between the two attenuation coefficients.

Kd(PAR)=0.6677Kd^0.6763(490).     

Long-range correlation properties of coding and noncoding DNA sequences: GenBank analysis

An open question in computational molecular biology is whether long-range correlations are present in both coding and noncoding DNA or only in the latter. To answer this question, we consider all 33301 coding and all 29453 noncoding eukaryotic sequences--each of length larger than 512 base pairs (bp)--in the present release of the GenBank to dtermine whether there is any statistically significant distinction in their long-range correlation properties. Standard fast Fourier transform (FFT) analysis indicates that coding sequences have practically no correlations in the range from 10 bp to 100 bp (spectral exponent beta=0.00 +/- 0.04, where the uncertainty is two standard deviations). In contrast, for noncoding sequences, the average value of the spectral exponent beta is positive (0.16 +/- 0.05) which unambiguously shows the presence of long-range correlations. We also separately analyze the 874 coding and the 1157 noncoding sequences that have more than 4096 bp and find a larger region of power-law behavior. We calculate the probability that these two data sets (coding and noncoding) were drawn from the same distribution and we find that it is less than 10(-10). We obtain independent confirmation of these findings using the method of detrended fluctuation analysis (DFA), which is designed to treat sequences with statistical heterogeneity, such as DNA's known mosaic structure ("patchiness") arising from the nonstationarity of nucleotide concentration. The near-perfect agreement between the two independent analysis methods, FFT and DFA, increases the confidence in the reliability of our conclusion.     

Exploring the genetic and environmental etiology of high general cognitive ability in fourteen- to thirty-six-month-old twins

Two definitions of normality ("isolated" or "correlated") are considered. The boundaries of "isolated" normality were determined by a statistical procedure, whereas the "correlated" approach was related to a clinical or predictive definition. In the latter case, the biological variations were considered abnormal if they implied a hazard with some significant future ailment as a risk factor. In this pragmatic approach, the upper limit of normal/abnormal variations is the point beyond which medical strategy is related to the most expected benefit when applied to a definite population or to an individual patient. The capacity of a diagnostic test to discriminate between patients with a defined risk and those without risk depends strictly on the value of the parameter chosen. In medical care for the prevention of vascular complications in diabetic patients or with foetal risks in pregnant women, the limits of the so-called normal range of glycaemia and other parameters should be determined according to the objective of the preventive and/or therapeutic measures to be prescribed.     

Changing patterns of localization of the tobacco mosaic virus movement protein and replicase to the endoplasmic reticulum and microtubules during infection

Tobacco mosaic virus (TMV) derivatives that encode movement protein (MP) as a fusion to the green fluorescent protein (MP:GFP) were used in combination with antibody staining to identify host cell components to which MP and replicase accumulate in cells of infected Nicotiana benthamiana leaves and in infected BY-2 protoplasts. MP:GFP and replicase colocalized to the endoplasmic reticulum (ER; especially the cortical ER) and were present in large, irregularly shaped, ER-derived structures that may represent "viral factories." The ER-derived structures required an intact cytoskeleton, and microtubules appeared to redistribute MP:GFP from these sites during late stages of infection. In leaves, MP:GFP accumulated in plasmodesmata, whereas in protoplasts, the MP:GFP was targeted to distinct, punctate sites near the plasma membrane. Treating protoplasts with cytochalasin D and brefeldin A at the time of inoculation prevented the accumulation of MP:GFP at these sites. It is proposed that the punctate sites anchor the cortical ER to plasma membrane and are related to sites at which plasmodesmata form in walled cells. Hairlike structures containing MP:GFP appeared on the surface of some of the infected protoplasts and are reminiscent of similar structures induced by other plant viruses. We present a model that postulates the role of the ER and cytoskeleton in targeting the MP and viral ribonucleoprotein from sites of virus synthesis to the plasmodesmata through which infection is spread.     

Multidisciplinary Simulation of the Maneuvering of an Aircraft

A computational methodology for the simulation of the transient aeroelastic response of an unrestrained and flexible aircraft during high-G maneuvers is presented. The key components of this methodology are: (a) a three-field formulation for coupled fluid/structure interaction problems; (b) a second-order time-accurate and geometrically conservative flow solver for CFD computations on unstructured dynamic meshes; (c) a corotational finite element method for the solution of geometrically nonlinear and unrestrained structural dynamics problems; (d) a robust method for updating an unrestrained and unstructured moving fluid mesh; and (e) a second-order time-accurate staggered algorithm for time-integrating the coupled fluid/structure semi-discrete equations of motion. This computational methodology is illustrated with the simulation on a parallel processor of several three-dimensional high-G pullup maneuvers of the Langley Fighter in the transonic regime, using a detailed finite element aeroelastic model.     

Turnover of inositol polyphosphate pyrophosphates in pancreatoma cells

There is little information concerning the intracellular function of inositol 1,3,4,5,6-pentakis- and hexakisphosphate, despite their being the most abundant inositol polyphosphates. Current opinions that they play passive roles as antioxidants (Graf, E., Mahoney, J. R., Bryant, R. G., and Eaton, J. W. (1987) J. Biol. Chem. 259, 3620-3624) or "housekeeping" molecules (Berridge, M. J., and Irvine, R. F. (1989) Nature 341, 197-205) arises from belief in their metabolic lethargy. However, we have discovered that cell homogenates, incubated with 5 mM fluoride and 5 mM ATP, converted both inositol hexakisphosphate (Km = 2 +/- 0.5 microM, Vmax = 9 +/- 2 pmol/mg of protein/min) and inositol 1,3,4,5,6-pentakisphosphate (Km = 13 +/- 4 microM, Vmax = 11 +/- 5 pmol/mg of protein/min) to more polar products. These reactions were also observed in intact cells treated with 0.5-20 mM fluoride, and the precursor/product relationships were confirmed by comparing the effects of fluoride on cells differentially labeled with [3H]inositol in either short-term or pulse-chase protocols. The novel products were determined to be inositol pyrophosphates because of their relatively specific hydrolysis by tobacco pyrophosphatase and alkaline phosphatase. The pyrophosphates were metabolized rapidly by cell homogenates back to their pentakisphosphate and hexakisphosphate precursors. This endogenous pyrophosphatase activity was inhibited by up to 99% by 5 mM fluoride in vitro. In intact cells incubated with 10 mM fluoride, about 20% of the inositol 1,3,4,5,6-pentakisphosphate pool, and 50% of the inositol hexakisphosphate pool were each converted to pyrophosphate derivatives within 1 h.