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51.
52.
Isotype-specific antibody responses to foot-and-mouth disease virus (FMDV) were measured in the sera and upper respiratory tract secretions of vaccinated and susceptible cattle challenged with FMDV by direct contact or by intranasal inoculation. A comparison was made between cattle that eliminated FMDV and those that developed and maintained a persistent infection. Serological and mucosal antibody responses were detected in all animals after challenge. IgA and IgM were detected before the development of IgG1 and IgG2 responses. IgM was not detected in vaccinated cattle. Challenge with FMDV elicited a prolonged biphasic secretory antibody response in FMDV "carrier' animals only. The response was detected as FMDV-specific IgA in both mucosal secretions and serum samples, which gained statistical significance (P < 0.05) by 5 weeks after challenge. This observation could represent the basis of a test to differentiate vaccinated and/or recovered convalescent cattle from FMDV "carriers'. 相似文献
53.
Alexander Stocker Alexander Richter Patrick Hoefler Klaus Tochtermann 《Computer Supported Cooperative Work (CSCW)》2012,21(2-3):317-356
The purpose of this paper is to provide both application-oriented researchers and practitioners with detailed insights into conception, implementation, and utilization of intra-organizational wikis to support knowledge management and group work. Firstly, we report on three case studies and describe how wikis have been appropriated in the context of a concrete practice. Our study reveals that the wikis have been used as Knowledge Base, Encyclopedia and Support Base, respectively. We present the identified practices as a result of the wiki appropriation process and argue that due to their open and flexible nature these wikis have been appropriated according to the users’ needs. Our contribution helps to understand how platforms support working practices that have not been supported by groupware before, or at least not in the same way. Secondly, three detailed implementation reports uncover many aspects of wiki projects, e.g., different viewpoints of managers and users, an investigation of other sources containing business-relevant information, and perceived obstacles to wiki projects. In this context, our study generates a series of lessons learned for people who intend to implement wikis in their own organizations, including the awareness of usage potential, the need for additional managerial support, and clear communication strategies to promote wiki usage. 相似文献
54.
Selective isolation of reversible cold sensitive variants from Chinese hamster ovary cell cultures 总被引:1,自引:0,他引:1
The fine structure and cellular associations of the large pigment cells (LPC's) of the compound eye of the house fly were studied with high voltage and conventional electron microscopy. Depending on the sector of the compound eye, the facets are either rectangular or hexagonal. The underside of each facet has indentations exactly aligned with those on top into which inserts an angulated sleeve of LPC's. Under the rectangular lens facet 6 or 8 small compact (in cross section) LPC's join four elongate LPC's. Clusters of compact cells alternate in this ring with elongate ones. Compact cells compress together and become quadrangular (in cross section) several microns below their insertion into the lens and form "building block" corners while elongate cells form "side rails" for the rectangular type of distal pseudocone enclosure. Beneath hexagonal facets all LPC's are rather elongate with out corner cells. In both facet types LPC's enclose the pseudocone for a longitudinal distance of 4 mum and then are displaced as bordering cells by a sleeve of two corneal pigment cells (CPC's), each of which encloses half of the proximal pseudocone. For the following 6 mum of longitudinal distance these concentric sleeves of CPC's and LPC's form a double layer around the pseudocone. At about 10 mum below lens base the two sleeves separate; LPC's become attenuated and extend cable-like to the basement membrane and CPC's enclose the proximal pseudocone, Semper cells and distal retinula. The junction between lens and LPC's has critical structural value in that (1) this is the sole anchorage to the lens by the lengthy remainder of the ommatidium, and (2) LPC's enclose the semiliquid pseudocone in the most distal portion of the pseudocone. In addition to vertical support, the LPC's send out numerous lateral processes that make structural contact among themselves, with the corneal pigment cells and the photoreceptor cells. The structural features of this array are discussed relative to possible physiological roles. 相似文献
55.
David Stocker et al. 《Vernacular Architecture》2013,44(1):40-65
Based on research undertaken by RUBL, a new building-recording group in Lincolnshire, this contribution originates in the observation that there are a very few standing timber-framed buildings in the county’s Middle Trent Valley. The paper is the first from a larger project exploring this absence. It addresses the suggestion that, although there were once similar numbers of vernacular buildings of this type as elsewhere in the north-east Midlands, this part of Lincolnshire was so greatly affected by a great rebuilding following enclosure between c. 1750 and c. 1850 that virtually all earlier box-framed structures were replaced in brick. Preliminary survey by the group suggests that this may be true, but it also reveals that some members of this earlier generation of box-framed buildings may survive disarticulated, as re-used timbers in those enclosure-period farmsteads that replaced them. Consequently, the group has undertaken a detailed analysis of the timbers re-used in the enclosure-period farmhouse at Thorpe-on-the-Hill in order to assess techniques for reconstructing the frames of predecessor buildings. Following extensive recording work, the outlines of two closely connected predecessor structures have been reconstructed and dated - through techniques including dendrochronology - to the mid-fifteenth and early sixteenth centuries. It is suggested that they were both ranges from the previous Manor Farm House, which occupied the same footprint as that still standing. 相似文献
56.
T. Schulze-König C. Maden E. Denk S.P.H.T. Freeman M. Stocker M. Suter H.-A. Synal T. Walczyk 《Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms》2010,268(7-8):752-755
A comparative study was made between the compact AMS system at the PSI/ETH Laboratory of Ion Beam Physics in Zurich with 0.5 MV terminal voltage and the 5 MV-AMS system at the Scottish Universities Environmental Research Centre (SUERC), Glasgow. Overall 34 urinary samples with 41Ca/40Ca ratios in the range from 4 × 10?11 to 3 × 10?10 were processed to CaF2 and aliquots of the same material were measured on both instruments.Measurements on the compact AMS system were performed in charge state 3+ achieving a transmission of 4% at 1.7 MeV beam energy. Under these conditions a suppression of the interference 41K is virtually impossible. However, samples with an excess of potassium can be identified by a shift of the 41Ca/41K peak in the ΔE ? E histogram of the gas ionization detector employed and a criterion for data rejection can be defined. An overall precision of ~4% and a 41Ca/40Ca background level of 5 × 10?12 have been reached.For studies with higher demands on the detection limit AMS systems like the one at SUERC are attractive: in charge state 5+ and using a gas stripper beam energy of 27 MeV, a transmission of 5%, a 41K suppression factor of ~500 and a 41Ca/40Ca background level of 3 × 10?14 are achieved.We demonstrate that both systems are well suited for large-scale 41Ca biomedical applications. 相似文献
57.
HJ Deeg W Leisenring R Storb J Nims ME Flowers RP Witherspoon J Sanders KM Sullivan 《Canadian Metallurgical Quarterly》1998,91(10):3637-3645
We reviewed the records and reevaluated 212 patients with aplastic anemia transplanted at the Fred Hutchinson Cancer Research Center (FHCRC) between 1970 and 1993 who survived >/=2 years and who have been followed for up to 26 years. Parameters analyzed included hematopoietic function, chronic graft-versus-host disease (GVHD), skin disease, cataracts, lung disease, skeletal problems, posttransplant malignancy, depression, pregnancy/fatherhood, and the return to work or school, as well as patient self-assessment of physical and psychosocial health, social interactions, memory and concentration, and overall severity of symptoms. Survival probabilities at 20 years were 89% for patients without (n = 125) and 69% for patients with chronic GVHD (n = 86) (the status was uncertain in 1 surviving patient). All patients had normal hematopoietic parameters. Skin problems occurred in 14%, cataracts in 12%, lung disease in 24%, and bone and joint problems in 18% of patients. Eleven patients (12%) developed a solid tumor malignancy and 19% of patients experienced depression. Chronic GVHD was the dominant risk factor for late complications. Seventeen patients died at 2.5 to 20.4 years posttransplant; 13 of these had chronic GVHD and related complications. At 2 years, 83% of patients had returned to school or work; the proportion increased to 90% by 20 years. At least half of the patients preserved or regained the ability to become pregnant or father children. Patients rated their quality of life as excellent and symptoms as minimal or mild. In conclusion, marrow transplantation in patients with aplastic anemia established long-term normal hematopoiesis. No new hematologic disorders occurred. The major cause of morbidity and mortality was chronic GVHD. However, the majority of patients who survived beyond 2 years returned to a fully functional life. 相似文献
58.
RJ Linnola RP Happonen OH Andersson E Vedel AU Yli-Urpo U Krause L Laatikainen 《Canadian Metallurgical Quarterly》1996,63(4):471-478
The current problem with keratoprosthesis is the ingrowth of corneal or conjunctival epithelium into the anterior chamber. This may lead to infections and extrusion of the prosthesis as well as to the development of retroprosthetic membrane and secondary glaucoma. Glass-ceramic coated and uncoated titanium has been tested as material for the keratoprosthesis to prevent epithelial ingrowth. Twenty-two Supra-Descemet's membrane keratoprostheses were inserted in the eyes of 22 rabbits for 1, 2, 4, 8, or 12 months. The prosthesis had an optic part made of polymethylmetacrylate (PMMA). The support for the optic part and the flange of the prosthesis were made of titanium. Eleven of the prostheses were coated with glass-ceramic. The histological sections of the enucleated eyes were prepared through the central part of the cornea and the prosthesis using a cutting-grinding method. The histological analysis was made on both halves of the implants separately giving two analysis areas in each eye. All 11 titanium prostheses were retained for the time period planned. Two glass-ceramic coated prostheses were lost at 2 and 4 weeks, respectively. This was caused by difficulties at surgery due to a thick coating. These eyes were excluded from the histological analysis. No significant ingrowth of epithelium was seen in 15/18 (83%) and in 16/22 (73%) of the analysed areas of the glass-ceramic coated and titanium prostheses, respectively. Titanium appears to be a suitable material for the keratoprosthesis. The ingrowth of the epithelium may be hindered further by coating the titanium with bioactive glass-ceramic. 相似文献
59.
60.
tau is a major component of paired helical filaments found in the neurofibrillary tangles of Alzheimer's diseased brain. However, the mechanism or mechanisms responsible for the association of tau to form these aggregates remains unknown. In this study, the role of intermolecular disulfide bonds in the formation of higher order oligomers of bovine tau and the human recombinant tau isoform T3 was examined using the chemical cross-linking agent disuccinimidylsuberate (DSS). In addition, the role of phosphorylation and oxidation state on the in vitro self-association of tau was studied using this experimental model. Stabilization of tau-tau interactions with DSS indicated that intermolecular disulfide bonds probably play a predominant role in dimer formation, but the formation of higher order oligomers of tau cannot be attributed to these bonds alone. tau-tau interactions were significantly decreased either by blocking Cys residues or by exposing the tau to a reducing (nitrogen and dithiothreitol), instead of an oxidizing, environment. tau self-association was also significantly decreased by prior phosphorylation with calcium/calmodulin-dependent protein kinase II. Phosphorylation by cyclic AMP-dependent protein kinase or dephosphorylation by alkaline phosphatase did not alter tau self-assembly. These data suggest a role for several factors that may modulate tau self-association in vivo. 相似文献