Abnormal tryptophan metabolism in a family with a history of bladder cancer

Tryptophan metabolism was studied in a family in which a sister and brother had bladder cancer. Urinary tryptophan metabolites after administration of 2 g L-tryptophan were measured in 4 sisters and 1 brother, all free of disease when studied. One sister with and 2 without histories of bladder cancer had significantly elevated levels of kynurenine, acetylkynurenine, and 3-hydroxykynurenine. Administration of 100 mg pyridoxine hydrochloride returned the tryptophan metabolism to normal in these 3 individuals. One brother with a 1 sister without bladder cancer had normal metabolism. A repeat study 2 years later confirmed the abnormal metabolism in the 3 sisters. Two sisters with abnormal tryptophan metabolism were given 200 mg L-kynurenine sulfate orally to bypass the effects of tryptophan oxygenase activity. Both excreted elevated levels of kynurenine and 1 excreted elevated levels of 3-hydroxykynurenine.     

The effects of chemical injury on the ocular surface

Chemical burns of the eye may destroy all of the corneal epithelium and large portions of conjunctival epithelium into the fornices. Restoration of the ocular surface after a chemical burn depends on the centritedal movement of conjunctival epithelial cells and their adherence to the altered corneal stroma. Epithelial movement after a corneal burn is normal for 72 hours after a burn, but persistent epithelial defects thereafter commonly are not resolved until total corneal vascularization occurs. Although this fresh epithelium may be protected by a bandage soft contact lens, more promising and far-reaching approaches may follow epithelial supplementation and even replacement. The link between the health and integrity of the epithelial layer as it relates to the corneal substratum and its cellular constituents remains to be forged.