Relationships Between Structure and Activity in the α-Amylase Family of Starch-metabolising Enzymes

α-Amylases are known to be multidomain proteins, i.e., the molecules consist of several folding units. Each α-amylase is believed, however, to have a catalytic domain consisting, of a barrel of eight parallel α-strands surrounded by eight α-strands. with an extra helix inserted after the sixth γ-strands. The α-strands and helices alternate along the polypeptide chain and are linked together by irregular loops. Amino acid residues situated on the loops joining the C-terminal end of each α-strand to the N-terminal end of the following helix make up the active site of the enzymes. A similar structure has been found in cyclodextrin glucanotransfcrases and it is now believed that such a (α/α)8-barrel also constitutes the catalytic domain of enzymes active on α-1.6-glucosidic bonds, and of enzymes with dual specificity for both α-1.4- and α-1.6- bonds. Knowledge of the three-dimensional structure of α-amylases and cyclodextrin glucanotransferase has made possible identification of structural features important for enzymic activity and specificity. By analogy, some general conclusions are reached concerning pullulanase, isoamylase. oligo-1,6-glucosidase, neopullulanase and branching enzymes.     

Calibrating the UHF technique of partial discharge detection usinga PD simulator

The paper describes a technique for simulating the presence of partial discharges in gas-insulated substation (GIS) equipment. The technique employs a pulse generator to inject current pulses through a suitable protrusion. The current pulses have similar temporal characteristics to those produced by an actual discharge event, with the added advantage that their magnitudes can be controlled accurately. Because the pulses are highly reproducible, they are ideally suited for investigating the UHF technique of partial discharge detection. The paper discusses the relationship between the magnitude of the UHF signal received by a coupler and the discharge magnitude. It also shows that the coupler signal is dependent on the protrusion length and on the spatial relationship between the protrusion and the coupler. The implications for practical discharge measurements in GIS also are discussed     

Dynamics of ammonia volatilization from simulated urine patches and aqueous urea applied to pasture. III. Field verification of a simplified model

Published field experimental data [11, 15, 19] were used to compare measured NH_3(g) losses following applications of urine or aqueous urea to pasture soils with values predicted by a simplified ammonia volatilization model [16]. Total measured losses were generally in close agreement with predictions. For example, predicted losses following applications of urine to a ryegrass-white clover pasture in Canterbury, New Zealand were 20.7% in summer and 22.4% in autumn and were highly correlated with measured losses of 21.5% and 24.4% respectively (r = 0.998).The model was also tested for instantaneous rate of ammonia gas loss at 33 discrete sampling times for the summer experiment. Correlations were again highly significant (r = 0.951 for urine and r = 0.885 for urea).The interception of urine solution by herbage and litter on the pasture surface is discussed and was shown to account for some of the discrepancies between measurements and predictions. Soil surface pH was confirmed as an important factor in determining the extent of ammonia gas loss, and the practicalities of measuring this parameter under field conditions are presented. It was concluded that the model offers the potential for predicting ammonia volatilization losses following urine or aqueous urea applications to short pasture in non-leaching, non-nitrifying environments.     

Influence of the number of questions on the student''s achievement in tests of anatomy

The authors compare student's achievement in tests with low and moderately high number of questions requiring short answers. Five groups of Medical School students underwent their regular Anatomy evaluation tests, which consisted of 13 questions (one group), 15 (two groups) and 25 (two groups). In each group five questions were chosen at random, average scores being calculated from their marks. These scores were statistically compared to the ones calculated from the marks given to the full-length evaluation tests; it became quite evidente that averages go up when the number of questions is increased. Putting the averages in terms of A, B, C and D scores (very good, good, regular and insufficient), it was noted that failure to reach at least the C score is inversely proportional to the number of questions. The results are discussed in terms of determining the most proper number of questions to be given in a test.