Protein-protein interactions in reversibly assembled nanopatterns

We describe herein a platform to study protein-protein interactions and to form functional protein complexes in nanoscopic surface domains. For this purpose, we employed multivalent chelator (MCh) templates, which were fabricated in a stepwise procedure combining dip-pen nanolithography (DPN) and molecular recognition-directed assembly. First, we demonstrated that an atomic force microscope (AFM) tip inked with an oligo(ethylene glycol) (OEG) disulfide compound bearing terminal biotin groups can be used to generate biotin patterns on gold achieving line widths below 100 nm, a generic platform for fabrication of functional nanostructures via the highly specific biotin-streptavidin recognition. Subsequently, we converted such biotin/streptavidin patterns into functional MCh patterns for reversible assembly of histidine-tagged (His-tagged) proteins via the attachment of a tris-nitriloacetic acid (trisNTA) biotin derivative. Fluorescence microscopy confirmed reversible immobilization of the receptor subunit ifnar2-His10 and its interaction with interferon-alpha2 labeled with fluorescent quantum dots in a 7 x 7 dot array consisting of trisNTA spots with a diameter of approximately 230 nm. Moreover, we carried out characterization of the specificity, stability, and reversibility as well as quantitative real-time analysis of protein-protein interactions at the fabricated nanopatterns by imaging surface plasmon resonance. Our work offers a route for construction and analysis of functional protein-based nanoarchitectures.     

Increasing the sustainability of steel production in the electric arc furnace by substituting fossil coal with biochar agglomerates

Biochar fines from a wood gasification plant and from pyrolysis of agricultural residues were investigated as substitutes for fossil coal used in the steel production in the electric arc furnace (EAF). During previous tests biochar fines with high specific surface showed problematic burn-off behaviour. Therefore the agglomeration behaviour of the biochar fines was investigated. Different binary and ternary mixtures of biochar with water and binders were tested in a hydraulic stamp press and evaluated with regard to green strength and fatigue strength of the briquettes after 3 days. One selected mixture was used to produce pillow briquettes in a double roll press. The abrasion behaviour of the produced briquettes was tested and compared to an anthracite reference coal (RC). Melting tests in a pilot EAF showed that the agglomerated biochar reacts similar to the RC. The briquetting leads to reduced reactivity and slower burn-off compared to the biochar fines.     

Functional Polysaccharide Composite Nanoparticles from Cellulose Acetate and Potential Applications

An in situ technique for preparing composite nanoparticles from hydrophobic cellulose acetate and hydrophilic polysaccharides using nanoprecipitation is presented. This technique allows the nanoparticles’ surface properties to be tuned very specifically. Spherical, narrow‐size‐distributed composite nanoparticles of different size, charge, functionality, and increased stability can be generated by using hydroxyethyl cellulose, carboxymethyl cellulose, low molecular weight chitosan, and amino cellulose. The influence of the pH and hydrophilic polysaccharide content in the particle formation is shown. The pH‐ and ionic strength‐ effective zeta‐potential functions are evidence of the presence of functional polysaccharides at the nanoparticle surface. The in situ technique is compared with the adsorption of hydrophilic polysaccharides onto cellulose acetate nanoparticles in two steps. The great potential of in situ prepared composite nanoparticles in the pharmaceutical industry and bio‐ or food technology, as carriers of hydrophobic substances in aqueous media and for specific surface modifications, e.g., to selectively introduce strong antimicrobial properties, is illustrated.     

Experimental Investigation of Rotating Menisci

In upper stages of spacecrafts, Propellant Management Devices (PMD’s) can be used to position liquid propellant over the outlet in the absence of gravity. Centrifugal forces due to spin of the upper stage can drive the liquid away from the desired location resulting in malfunction of the stage. In this study, a simplified model consisting of two parallel, segmented and unsegmented disks and a central tube assembled at the center of the upper disk is analyzed experimentally during rotation in microgravity. For each drop tower experiment, the angular speed caused by a centrifugal stage in the drop capsule is kept constant. Steady-states for the menisci between the disks are observed for moderate rotation. For larger angular speeds, a stable shape of the free surfaces fail to sustain and the liquid is driven away. Additionally, tests were performed without rotation to quantify two effects: the removal of a metallic cylinder around the model to establish the liquid column and the determination of the the settling time from terrestrial to microgravity conditions.     

Beschreibung der Fließkurve auf der Basis phänomenologischer Ansätze am Beispiel eines Baustahls

Zur Beschreibung der Fließkurve des untersuchten Baustahls wurde ein allgemeines phänomenologisches Modell zur Entwicklung der Gesamtversetzungsdichte mit der plastischen Vergleichsdehnung herangezogen. Spezielle Formen dieser Evolutionsgleichung liefern bekannte empirische Fließgesetze. Unter Berücksichtigung der Randbedingungen bei der Herleitung dieser Gesetze sind deren Koeffizienten physikalisch plausibel zu interpretieren. Der gebräuchlichen Hollomon-Gleichung ähnliche Potenzfunktionen, die eine additive Konstante enthalten, sind auf der Basis dieser Modellvorstellung als Fließgesetze ohne Berücksichtigung von Erholungsprozessen zu verstehen. Die Güte der Approximation ist folglich stark von der Versuchstemperatur abhängig. Die Voce-Gleichung stellt die exakteste Beschreibung der Fließkurve des untersuchten Stahls dar. Aufgrund der Berücksichtigung von Erholungsvorgängen enthält die Voce-Gleichung, im Gegensatz zu den anderen Ansätzen, eine Grenz- oder Sättigungsspannung, deren Temperaturabhängigkeit unter Zugrundelegung eines thermisch aktivierten Versetzungsprozesses quantifiziert werden kann. Abweichungen am Beginn homogener plastischer Verformung sind auf die Wirkung eines zweiten Strukturparameters, der Dichte der beweglichen Versetzungen zurückzuführen. Mit Hilfe neuerer Modellvorstellungen kann dieser Bereich quantitativ beschrieben werden.     

Immunological assessment of exposure to Echinococcus granulosus in a rural dog population in Uruguay

An ELISA was used to screen a dog population in Uruguay (Sarandi Del Yi, Durazno District) for the prevalence of specific serum antibodies (IgG, IgA and IgE) to Echinococcus granulosus. The sensitivity (61%) and specificity (97%) of the ELISA were determined using well-defined serum groups. A total of 408 dogs from Sarandi del Yi and environs were screened serologically, and 29.7% (8.6-13.8% for each antibody class) of dogs had positive levels of antibody to E. granulosus. This antibody prevalence (exposure) was significantly higher than the percentage of dogs found to be positive for E. granulosus worms by arecoline purgation (7.6%). This level of exposure to E. granulosus determined by ELISA is considered unacceptable from a public health perspective. Measures will now focus on obtaining data on the true prevalence of current infection in this dog population and on determining the transmission patterns of the disease in this endemic region.