1mm内径HPLC整体柱用于蛋白质的高效分离

介绍了1mm内径的聚苯乙烯-二乙烯基苯整体柱用于液相色谱反相梯度高效分离完整的蛋白质。文中详细研究了柱温、流速和梯度时间等对峰宽和峰容量的影响。在最优色谱条件下该柱高效地反相分离了核糖核酸酶A、肌红蛋白和碳酸酐酶的蛋白质混合物以及大肠杆菌提取蛋白。     

Development of Tyrphostin Analogues to Study Inhibition of the Mycobacterium tuberculosis Pup Proteasome System**

Tuberculosis is a global health problem caused by infection with the Mycobacterium tuberculosis (Mtb) bacteria. Although antibiotic treatment has dramatically reduced the impact of tuberculosis on the population, the existence and spreading of drug resistant strains urgently demands the development of new drugs that target Mtb in a different manner than currently used antibiotics. The prokaryotic ubiquitin-like protein (Pup) proteasome system is an attractive target for new drug development as it is unique to Mtb and related bacterial genera. Using a Pup-based fluorogenic substrate, we screened for inhibitors of Dop, the Mtb depupylating protease, and identified I-OMe-Tyrphostin AG538 ( 1 ) and Tyrphostin AG538 ( 2 ). The hits were validated and determined to be fast-reversible, non-ATP competitive inhibitors. We synthesized >25 analogs of 1 and 2 and show that several of the synthesized compounds also inhibit the depupylation actions of Dop on native substrate, FabD-Pup. Importantly, the pupylation activity of PafA, the sole Pup ligase in Mtb, was also inhibited by some of these compounds.     

Interactive rodent behavior annotation in video using active learning

Multimedia Tools and Applications - Manual annotation of rodent behaviors in video is time-consuming. By learning a classifier, we can automate the labeling process. Still, this strategy requires a...     

Multimedia Algorithmics

If perceptually relevant multimedia methods with guaranteed performance are not developed soon, there is no hope that the problem of multimedia information overload is effectively solved. Research is needed to handle images, music, video, and 3D models, with methods that guarantee robustness, invariance, efficiency, etc., and are also perceptually and cognitively relevant. The invention of algorithms that provably satisfy such properties is a new field of research: multimedia algorithmics.     

iPCA: An Interactive System for PCA-based Visual Analytics

Principle Component Analysis (PCA) is a widely used mathematical technique in many fields for factor and trend analysis, dimension reduction, etc. However, it is often considered to be a "black box" operation whose results are difficult to interpret and sometimes counter-intuitive to the user. In order to assist the user in better understanding and utilizing PCA, we have developed a system that visualizes the results of principal component analysis using multiple coordinated views and a rich set of user interactions. Our design philosophy is to support analysis of multivariate datasets through extensive interaction with the PCA output. To demonstrate the usefulness of our system, we performed a comparative user study with a known commercial system, SAS/INSIGHT's Interactive Data Exploration. Participants in our study solved a number of high-level analysis tasks with each interface and rated the systems on ease of learning and usefulness. Based on the participants' accuracy, speed, and qualitative feedback, we observe that our system helps users to better understand relationships between the data and the calculated eigenspace, which allows the participants to more accurately analyze the data. User feedback suggests that the interactivity and transparency of our system are the key strengths of our approach.     

Spark Discharge Doping—Achieving Unprecedented Control over Aggregate Fraction and Backbone Ordering in Poly(3-hexylthiophene) Solutions

The properties of semiconducting polymers are strongly influenced by their aggregation behavior, that is, their aggregate fraction and backbone planarity. However, tuning these properties, particularly the backbone planarity, is challenging. This work introduces a novel solution treatment to precisely control the aggregation of semiconducting polymers, namely current-induced doping (CID). It utilizes spark discharges between two electrodes immersed in a polymer solution to create strong electrical currents resulting in temporary doping of the polymer. Rapid doping-induced aggregation occurs upon every treatment step for the semiconducting model-polymer poly(3-hexylthiophene). Therefore, the aggregate fraction in solution can be precisely tuned up to a maximum value determined by the solubility of the doped state. A qualitative model for the dependences of the achievable aggregate fraction on the CID treatment strength and various solution parameters is presented. Moreover, the CID treatment can yield an extraordinarily high quality of backbone order and planarization, expressed in UV–vis absorption spectroscopy and differential scanning calorimetry measurements. Depending on the selected parameters, an arbitrarily lower backbone order can be chosen using the CID treatment, allowing for maximum control of aggregation. This method may become an elegant pathway to finely tune aggregation and solid-state morphology for thin-films of semiconducting polymers.     

Multicore Liquid Perfluorocarbon‐Loaded Multimodal Nanoparticles for Stable Ultrasound and 19F MRI Applied to In Vivo Cell Tracking

Ultrasound is the most commonly used clinical imaging modality. However, in applications requiring cell‐labeling, the large size and short active lifetime of ultrasound contrast agents limit their longitudinal use. Here, 100 nm radius, clinically applicable, polymeric nanoparticles containing a liquid perfluorocarbon, which enhance ultrasound contrast during repeated ultrasound imaging over the course of at least 48 h, are described. The perfluorocarbon enables monitoring the nanoparticles with quantitative ¹⁹F magnetic resonance imaging, making these particles effective multimodal imaging agents. Unlike typical core–shell perfluorocarbon‐based ultrasound contrast agents, these nanoparticles have an atypical fractal internal structure. The nonvaporizing highly hydrophobic perfluorocarbon forms multiple cores within the polymeric matrix and is, surprisingly, hydrated with water, as determined from small‐angle neutron scattering and nuclear magnetic resonance spectroscopy. Finally, the nanoparticles are used to image therapeutic dendritic cells with ultrasound in vivo, as well as with ¹⁹F MRI and fluorescence imaging, demonstrating their potential for long‐term in vivo multimodal imaging.