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201.
In vitro studies toward noninvasive glucose monitoring with optical coherence tomography 总被引:1,自引:0,他引:1
We use optical coherence tomography (OCT) to measure glucose-induced changes in Intralipid and in mouse skin samples in vitro. Mouse skin samples are cultured in a CO2 incubator before measurements are made with different amounts of added glucose concentrations. The results show that the glucose-induced changes in the OCT slope value vary between 20% and 52%/30 mM glucose in different mouse skin samples. This change is much larger than the change in 2% Intralipid (2.1%/30 mM) and in 5% Intralipid (0.86%/30 mM). Hence the results show that OCT has potential to monitor glucose-induced changes in tissues in vitro. 相似文献
202.
Ostman P Luosujärvi L Haapala M Grigoras K Ketola RA Kotiaho T Franssila S Kostiainen R 《Analytical chemistry》2006,78(9):3027-3031
An atmospheric pressure chemical ionization (APCI) microchip is presented for combining a gas chromatograph (GC) to a mass spectrometer (MS). The chip includes capillary insertion channel, stopper, vaporizer channel, nozzle and nebulizer gas inlet fabricated on the silicon wafer, and a platinum heater sputtered on a glass wafer. These two wafers are joined by anodic bonding creating a two-dimensional version of an APCI microchip. The sample from GC is directed via heated transfer line capillary to the vaporizer channel of the APCI chip. The etched nozzle forms narrow sample plume, which is ionized by an external corona discharge needle, and the ions are analyzed by a mass spectrometer. The GC-microchip APCI-MS combination provides an efficient method for qualitative and quantitative analysis. The spectra produced by microchip APCI show intensive protonated molecule and some fragmentation products as in classical chemical ionization for structure elucidation. In quantitative analysis the GC-microchip APCI-MS showed good linearity (r(2) = 0.9989) and repeatability (relative standard deviation 4.4%). The limits of detection with signal-to-noise ratio of three were between 0.5 and 2 micromol/L with MS mode using selected ion monitoring and 0.05 micromol/L with MS/MS using multiple reaction monitoring. 相似文献
203.
Efficient Reinforcement Learning through Symbiotic Evolution 总被引:13,自引:0,他引:13
This article presents a new reinforcement learning method called SANE (Symbiotic, Adaptive Neuro-Evolution), which evolves a population of neurons through genetic algorithms to form a neural network capable of performing a task. Symbiotic evolution promotes both cooperation and specialization, which results in a fast, efficient genetic search and discourages convergence to suboptimal solutions. In the inverted pendulum problem, SANE formed effective networks 9 to 16 times faster than the Adaptive Heuristic Critic and 2 times faster than Q-learning and the GENITOR neuro-evolution approach without loss of generalization. Such efficient learning, combined with few domain assumptions, make SANE a promising approach to a broad range of reinforcement learning problems, including many real-world applications. 相似文献
204.
Onni Niemel Anni S. Halkola Aini Bloigu Risto Bloigu Ulla Nivukoski Heidi Pohjasniemi Johanna Kultti 《International journal of molecular sciences》2022,23(21)
Aberrations in blood cells are common among heavy alcohol drinkers. In order to shed further light on such responses, we compared blood cell status with markers of hemolysis, mediators of inflammation and immune responses to ethanol metabolites in alcohol-dependent patients at the time of admission for detoxification and after abstinence. Blood cell counts, indices of hemolysis (LDH, haptoglobin, bilirubin), calprotectin (a marker of neutrophil activation), suPAR, CD163, pro- and anti-inflammatory cytokines and autoantibodies against protein adducts with acetaldehyde, the first metabolite of ethanol, were measured from alcohol-dependent patients (73 men, 26 women, mean age 43.8 ± 10.4 years) at baseline and after 8 ± 1 days of abstinence. The assessments also included information on the quantities of alcohol drinking and assays for biomarkers of alcohol consumption (CDT), liver function (AST, ALT, ALP, GGT) and acute phase reactants of inflammation. At baseline, the patients showed elevated values of CDT and biomarkers of liver status, which decreased significantly during abstinence. A significant decrease also occurred in LDH, bilirubin, CD163 and IgA and IgM antibodies against acetaldehyde adducts, whereas a significant increase was noted in blood leukocytes, platelets, MCV and suPAR levels. The changes in blood leukocytes correlated with those in serum calprotectin (p < 0.001), haptoglobin (p < 0.001), IL-6 (p < 0.02) and suPAR (p < 0.02). The changes in MCV correlated with those in LDH (p < 0.02), MCH (p < 0.01), bilirubin (p < 0.001) and anti-adduct IgG (p < 0.01). The data indicates that ethanol-induced changes in blood leukocytes are related with acute phase reactants of inflammation and release of neutrophil calprotectin. The studies also highlight the role of hemolysis and immune responses to ethanol metabolites underlying erythrocyte abnormalities in alcohol abusers. 相似文献