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Jirina Tyleckova Rita Hrabakova Katerina Mairychova Petr Halada Lenka Radova Petr Dzubak Marian Hajduch Suresh J. Gadher Hana Kovarova 《International journal of molecular sciences》2012,13(12):15536-15564
A comprehensive proteome map of T-lymphoblastic leukemia cells and its alterations after daunorubicin, doxorubicin and mitoxantrone treatments was monitored and evaluated either by paired comparison with relevant untreated control and using multivariate classification of treated and untreated samples. With the main focus on early time intervals when the influence of apoptosis is minimized, we found significantly different levels of proteins, which corresponded to 1%–2% of the total amount of protein spots detected. According to Gene Ontology classification of biological processes, the highest representation of identified proteins for all three drugs belong to metabolic processes of proteins and nucleic acids and cellular processes, mainly cytoskeleton organisation and ubiquitin-proteasome pathway. Importantly, we observed significant proportion of changes in proteins involved in the generation of precursor metabolites and energy typical for daunorubicin, transport proteins participating in response to doxorubicin and a group of proteins of immune system characterising response to mitoxantrone. Both a paired comparison and the multivariate evaluation of quantitative data revealed daunorubicin as a distinct member of the group of anthracycline/anthracenedione drugs. A combination of identified drug specific protein changes, which may help to explain anti-cancer activity, together with the benefit of blocking activation of adaptive cancer pathways, presents important approaches to improving treatment outcomes in cancer. 相似文献
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Universal Access in the Information Society - Today the Internet is the easiest way to find information about any kind of organization, and the first impression about an organization is almost... 相似文献
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Liliana G. Fidalgo Mauro D. Santos Rui P. Queirós Rita S. Inácio Maria J. Mota Rita P. Lopes Mafalda S. Gonçalves Ricardo F. Neto Jorge A. Saraiva 《Food and Bioprocess Technology》2014,7(7):2028-2037
Hyperbaric storage at naturally variable room temperature (RT) conditions (18–21 °C) and above (30 °C) was evaluated as a possible new food preservation method, regardless of temperature. Preservation of watermelon juice (used as a case study of a highly perishable food) at RT and 5 °C at atmospheric pressure was compared to preservation under 100 MPa at RT. After 8 h of hyperbaric storage at 100 MPa, the initial microbial loads of the watermelon juice were reduced by 1 log unit for total aerobic mesophiles, and 1–2 log units for Enterobacteriaceae and yeasts and moulds, to levels of about 3 log units for the former and below the detection limit for the latter, and remained thereafter unchanged up to 60 h. Similar results were obtained at 30 °C at 100 MPa after 8 h. At atmospheric pressure at RT (24 h) and 30 °C (8 h), microbial levels were already above quantification limits and unacceptable for consumption. Furthermore, pressure attenuated the increase in titratable acidity verified at atmospheric pressure, but caused higher colour changes, especially a higher lightness and a lower browning degree. Post-hyperbaric storage at 5 °C revealed an extended shelf life, as an additional benefit of hyperbaric storage. These results show that hyperbaric storage is a very promising food preservation methodology. 相似文献
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Shaveta Sharma Rita Sharma Praveen Kumar R. Thangaraj K. Asokan M. Mian 《Journal of Materials Science: Materials in Electronics》2017,28(19):14202-14208
The study of steady state and transient photocurrent measurement provide important information about carrier generation and recombination phenomena in various semiconducting systems for photo-sensor device applications. In the present work, the composition dependent analysis of photocurrents was studied for thermally evaporated Se-rich InxSb30?xSe70 films of average thickness 800 nm. The indirect optical gap has been calculated from the transmission and reflection data and the variation of molecular units was studied from the Raman spectroscopy. The initial rise of photocurrent sharply to approach a steady state value during illumination and fast decay to a constant persistent current after stopping the illumination has been observed. The intensity dependence of photocurrent obeys the power law IPh?=?Fγ, where the value of exponent tells about the recombination process. The decay of photocurrent has been fitted with stretched exponential function for different compositions and at different light intensities. These results are important for the development of low cost photo absorbers for solar cell applications and visible region responsive photo sensor devices. 相似文献
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Francesco Lolli Rita Gamberini Claudio Giberti Bianca Rimini Federica Bondi 《国际生产研究杂志》2016,54(14):4228-4239
In accordance with the lean production philosophy, an assembly line may be supplied by means of a kanban system, which regulates and simplifies the flow of materials between the lines and the warehouses. This paper focuses on evaluation of feeding policies that differ from each other in term of the number of kanbans managed per feeding tour. A pure cost-based approach is thus proposed, which considers both inline inventories along with handling costs proportionate to the number of operators involved in the parts-feeding process. A multi-scenario simulative approach is applied in order to establish the number of operators required to avoid inline shortages. The scenario minimising total cost is then selected. The innovation introduced is a model for describing kanban arrivals and their requests for feeding, improving the potential of the simulation to describe real-life environments. Lastly, a case study from the automotive industry is presented in order to highlight the applicability of the proposed approach as well and the effects of alternative feeding policies on the total cost incurred. 相似文献
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Lia-Tânia Dinis Maria Manuela OliveiraJosé Almeida Rita CostaJosé Gomes-Laranjo Francisco Peixoto 《Food chemistry》2012,132(1):1-8
The antioxidant properties of different ecotypes of chestnut nut (cv. Judia) were studied. Total phenolics and flavonoids were also determinated. Total phenolics amount ranged from 9.6 mg/g of GAE (hottest ecotype, Murça) to 19.4 mg/g of GAE (coldest ecotype, Valpaços). Gallic and ellagic acid were the predominant compounds and Valpaços had the highest values while, Murça had the lowest ones. The antioxidant capacity of ethanolic extracts were evaluated through several biochemical essays: ABTS (2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid)) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical-scavenging activity, FRAP (ferric reducing/antioxidant power) and inhibition of oxidative haemolysis in erythrocytes. In order to evaluate the antioxidant efficiency of each ecotype, the EC50 values were calculated. Once again Valpaços revealed the best antioxidant properties, presenting much lower EC50 values. Climatic conditions influence seems to be a limiting factor for production of phenolic compounds and consequently for the antioxidant properties of chestnut nuts. 相似文献