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91.
To investigate the feasibility of using tissue obtained from human tumor xenografts for in vitro screening of antineoplastic agents, we grew human tumor colony-forming units (CFU) in semisold agar from xenografts serially passaged in nude mice. Growth of human tumor CFU was accomplished from nine xenografts representing five different histological tumor types (ovarian carcinoma, adenocarcinoma of the colon, malignant melanoma, epidermoid carcinoma of the lung, and malignant astrocytoma). Cloning efficiency ranged from 0.04 to 0.1% and showed significant variability both between tumor types and between individual animals bearing the same type of xenograft. A high percentage of tumor CFU was in S phase [47 +/- 20% (S.D.)] as determined by the thymidine "suicide" technique. The number of tumor CFU observed increased linearly with increasing numbers of cells plated. In vitro drug sensitivity of the tumor CFU was assessed to Adriamycin, cis-platinum, and melphalan. The patterns of drug sensitivity were found to be reproducible and stable over a period of 9 months. Drug sensitivity curves to Adriamycin for five xenografts representing four tumor types showed complex patterns with plateau portions similar to those described for tumor CFU from primary tumors. The rank order of sensitivity of the tumors was compared to that of normal granulocyte-macrophage progenitors and, with the exception of the melanomas, was found to correlate well with clinical experience (order of sensitivity = colon less than ovary less than bone marrow). Growth of human tumor CFU from xenografts represents a reproducible and stable means for the study of the biology of tumor CFU and has potential applications as a means for screening new anticancer agents. 相似文献
92.
Hooshang Hemami Kamran Barin Yi-Chung Pai 《IEEE transactions on neural systems and rehabilitation engineering》2006,14(4):470-480
The ankle strategy is one of the postural adjustment maneuvers humans utilize when the support platform is disturbed. This paper presents a quantitative analysis of the ankle strategy. A three-link sagittal biped model is considered. The first link represents the two legs locked together. The second link represents the two thighs locked together. The third link represents the hip, the torso, the upper limbs, the neck, and the head. The dynamics, control, and stability of the three-link biped, under platform translation, are considered. The disturbance of the platform is represented as an input and the effect of the muscular system is reduced to a set of torques applied to the joints and across the joints. Two digital computer simulations are presented to demonstrate the behavior of the biped under backward or forward platform disturbance. The simulations are compared with experimental measurements of humans subjected to postural disturbances. It is shown that the effect of a horizontal disturbance at the ankle appears to be about 40 times that of the effect of the disturbance at the knees and at least a few hundred times larger than the effect of a disturbance at the hip. This means that, under translational platform disturbance, the ankle angle is subjected to the largest excursion. The knee and the hip angle excursions are relatively minor. Consequently, the biped, as a whole, appears to move as a single inverted pendulum. Major postural corrections are initiated by the ankle excursion. Further, when the available ankle torque is limited or nonexistent, the stability requires resorting to the knee or hip strategies. 相似文献
93.
基于割集分析与回路分析的撕裂方程直接生成方法,是一咱能避免撕裂方程生成过程中大量阵运算的一种方法,具有计算简单、便于编制计算程序的特点。 相似文献
94.
Ji Young Hyun Cheol Wan Park Yanna Liu Daeun Kwon Seong‐Hyun Park Sungjin Park Jaeyoung Pai Prof. Dr. Injae Shin 《Chembiochem : a European journal of chemical biology》2017,18(12):1077-1082
Fifty‐five mono‐ and disaccharide analogues were prepared and used for the construction of microarrays to uncover lectin‐selective ligands. The microarray study showed that two disaccharide analogues, 28′ and 44′ , selectively bind to Solanum tuberosum lectin (STL) and wheat germ agglutinin (WGA), respectively. Cell studies indicated that 28′ and 44′ selectively block the binding of STL and WGA to mammalian cells, unlike the natural ligand LacNAc, which suppresses binding of both STL and WGA to cells. 相似文献
95.
Mao-Yuan Pai Hui-Chuan Chu Su-Chen Wang Yuh-Min Chen 《Expert systems with applications》2013,40(6):1993-2006
Since eWOM provides a rich source of objective information about products or services, it has become one of the major ways in which consumers collect information about items they are interested in buying. However, the problem of eWOM overload makes it difficult to effectively collect this information, and may have adverse effects on their actual purchase behavior. eWOM content is characterized by unstructured text formats, oversimplified expressions, and newly coined phrases (textspeak), and these all contribute to the challenges that arise when analyzing eWOM. This study thus proposes an eWOM analysis method for analyzing eWOM, which may lead to a more effective method for analyzing eWOM content, extracting both positive and negative appraisals, and help consumers in their decision making. At the same time, the method proposed in this study can also be utilized as a tool to assist companies in better understanding product or service appraisals, thus translating these opinions into business intelligence and as the basis for product/service improvements. 相似文献
96.
Abstract Recently, Gao and Tu presented an efficient algorithm for robust low bit‐rate video transmission by using a partial backward decodable bit stream (PBDBS) approach. In this paper, we first present a multiple‐PBDBS (MPBDBS) approach to improve on the previous PBDBS approach. Next a mathematical theory is provided to minimize the error propagation length in each group of blocks (GOB). Further, a novel MPBDBS‐based algorithm is presented for robust video transmission. Experimental results demonstrate that our proposed MPBDBS‐based algorithm has better image quality when compared to the previous PBDBS‐based algorithm, but has some bit‐rate and execution‐time degradation. In our experiments, both single and two‐bit error models are investigated. 相似文献
97.
98.
Ashish Nadar Atindra Mohan Banerjee M.R. Pai R.V. Pai Sher Singh Meena R. Tewari A.K. Tripathi 《International Journal of Hydrogen Energy》2018,43(1):37-52
Supported iron oxides have been established as an important class of catalyst for high temperature sulfuric acid decomposition. With an objective to elucidate the role of support in modifying the overall catalytic properties of dispersed iron oxide catalysts, a series of supported iron oxide based catalysts, Fe2O3 (15 wt%)/MO2 (M = Zr, Ce, Ti and Si), synthesized by adsorption-equilibrium method, is investigated for sulfuric acid decomposition reaction. The structure of dispersed iron oxide phases largely depended on the nature of the support oxide as revealed by the XRD and Mössbauer studies. α-Fe2O3 is found to be present as a major phase on ZrO2 and CeO2 support while ε-Fe2O3 was the major phase on silica supported iron oxide. On the other hand, presence of mixed oxide Fe2TiO5 was revealed over TiO2 support. Strong dispersed metal oxide-support interactions inhibited the total reduction of the dispersed phase on SiO2 and TiO2 as compared to complete reduction of dispersed iron oxide on CeO2 and ZrO2 supports during temperature programmed reduction upto 1000 °C. The order of catalytic activity at a temperature of ~750 °C is observed as Fe2O3/SiO2 > Fe2TiO5/TiO2 > Fe2O3/ZrO2 > Fe2O3/CeO2, while at higher temperatures of ~900 °C the SO2 yield is found to be comparable for all catalysts. A relationship between the rate of sulfate decomposition and catalytic activity is established through detailed TG-DTA investigations of sulfated catalyst and support. Considerable influence of the support oxide on the composition, structure, redox properties, morphology and catalytic activities of the active iron oxide dispersed phase has been observed. Thus, the support oxides operate as a critical component in the complex supported metal oxide catalysts and these findings might influence the design and development of future high temperature sulfuric acid decomposition catalysts. 相似文献
99.
Pei-Yan Pai 《Expert systems with applications》2012,39(1):154-161
In this paper, a nucleus and cytoplast contour detector (NCC detector) is presented to automatically detect the cytoplast and nucleus contours of a cell in a cervical smear image. The NCC detector uses the adaptable threshold decision (ATD) method to separate the cell from the cervical smear image, and then uses the maximal gray-level-gradient-difference (MGLGD) method, proposed in this paper, to extract the nucleus from the cell. The experimental results show that the NCC detector is superior to two existing methods, the gradient vector flow-active contour model (GVF-ACM) and the edge enhancement nucleus and cytoplast contour (ENNCC) detector, in segmenting the cytoplast and nucleus of a cell. 相似文献
100.
Yan-Hom Li Ching-Yao Chen Shih-Tsung Sheu Jay-Min Pai 《Microfluidics and nanofluidics》2012,13(4):579-588
The dynamics of microchains containing superparamagnetic particles in an oscillating field are studied experimentally. The chains are first formed by a static directional field, and then manipulated by an additional dynamical perpendicular field. The present methodology represents a simple reversible chaining process, whose particles can be re-dispersed after removal of the field. The motion of superparamagnetic chains is dominated by magnetic torque and induced hydrodynamic drag. The effects of key parameters, such as field strengths and the lengths of particle chains, are thoroughly analyzed. Distinct behaviors, from rigid body oscillations and bending distortions to rupture failures, are observed by increasing the amplitudes of oscillating fields or chains’ lengths. Because of lower induced drag, a shorter chain follows the field trajectory closely and oscillates more synchronically with the external field. On the other hand, the influences of field strengths are not consistent. Even the overall oscillating phase trajectory in a stronger external field deviates less significantly from the corresponding field trajectory, a stronger dynamical component of the external field results in larger phase angle lags at certain points. The experimental results confirm the criterion of ruptures can be effectively determined by the value of (N*Mn 1/2), where Mn is the Mason number defined as the ratio of induced drag to dipolar attraction, and N represents the number of particles contained in a chain. 相似文献