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H Kook JH Rhee SE Lee SY Kang SS Chung KW Cho YH Baik 《Canadian Metallurgical Quarterly》1999,365(2-3):267-272
Recently we reported that Vibrio vulnificus hemolysin, an exotoxin produced by V. vulnificus, dilates rat thoracic aorta via elevated cGMP levels without affecting nitric oxide synthase. We investigated the mechanism further by observing the guanylyl cyclase activities in cytosolic, membrane, unfractionated, or reconstituted preparations. Hemolysin did not activate guanylyl cyclase in the membrane or cytosolic fraction, while it activated guanylyl cyclase in unfractionated or reconstituted preparation. The increased activity was not inhibited by the HS-142-1, a microbial polysaccharide which antagonizes atrial natriuretic peptide receptor, or 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), a soluble guanylyl cyclase inhibitor. However, it was attenuated by 6-(phenylamino)-5,8-quinolinedione (LY 83.583), which inhibits the catalytic domain of both guanylyl cyclases, and by cholesterol, which blocks hemolysin-incorporation into the membrane. Removing ATP, a cofactor of particulate guanylyl cyclase, attenuated the activation and ATPgammaS, a non-phosphorylating analog, restored it. These results suggest that V. vulnificus hemolysin activates particulate guanylyl cyclase via hemolysin incorporation into the vascular smooth muscle cell membrane in cooperation with certain unidentified cytosolic component(s). 相似文献
123.
This research was conducted to teach two adults with schizophrenia and mental retardation to respond to recorded audio prompts in order to eliminate the need for instructor assistance in completing routine prevocational tasks. Studying individuals with dual diagnosis is an important step in moving toward success in community living and vocational placement. A multiple probe design across tasks was conducted. Prior to the investigation, both individuals demonstrated low levels of independent task completion. Following the implementation of the audio prompts, both individuals' task completion performances dramatically increased. These findings suggest that audio prompts may serve as an efficient alternative to instructor promoting, which is often required by individuals with dual diagnosis in prevocational job settings. 相似文献
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J. A. Delgado Atencio E. E. Orozco Guillén S. Vázquez y Montiel M. Cunill Rodríguez J. Castro Ramos J. L. Gutiérrez F. Martínez 《Optical Memory & Neural Networks》2009,18(1):6-14
In a routine procedure for collecting human skin spectra using fiber optic diffuse reflectance spectroscopy, the fiber optic
probe is put gently in contact with the skin surface. The purpose of this work is to investigate the influence of probe pressure
on in-vivo diffuse reflectance spectroscopy measurements performed by a non-trained operator. In addition, we include simulation using
diffusion theory that explains the behavior of diffuse reflectance spectra with the increase of probe pressure.
The article is published in the original. 相似文献
125.
SE Hiscox MB Hallett MC Puntis T Nakamura WG Jiang 《Canadian Metallurgical Quarterly》1997,15(6):513-521
To characterize the clinical features of childhood tuberculosis, we analyzed the symptoms, signs, and laboratory findings of the 89 children with tuberculosis admitted to the Yokohama City University Hospital from 1975 to 1994. Compared with the numbers of patients admitted from 1975 to 1979, those of patients of the past 5 years (from 1990 to 1994) were reduced by half. Of the 89 subjects, 56.2% were below 3 years of age and 24.7% were under 1 year of age. 51.7% had primary complex and 20.2% had serious tuberculosis (tuberculous meningitis 14.6%, miliary tuberculosis 3.4%, and bone and joint tuberculosis 2.2%). Tuberculous children below 3 years of age consisted of primary complex (60.0%) and serious tuberculosis (32.0%). The majority (86.0%) of tuberculous children below 3 years of age had not received BCG vaccination. In 55 (61.8%) of 89 subjects, the sources of tuberculosis were clarified. Of these subjects, 83.6% were infected in the family. The rate of BCG inocluation tended to decrease with decreasing age, especially that of children below 3 years of age was 14.0%. Of the 89 subjects, only 16.9% proved to be smear-positive. Taken together, in order to eliminate tuberculous children below 3 years of age, the following is necessary; (1) BCG inoculation in early infancy, (2) early diagnosis of index cases with adult tuberculosis, and (3) prompt and appropriate family contact examination. 相似文献
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