Perinuclear antineutrophil cytoplasmic antibodies in patients with Crohn's disease define a clinical subgroup

BACKGROUND & AIMS: Antineutrophil cytoplasmic antibodies (ANCA) have been consistently detected in a subgroup of patients with Crohn's disease (CD). This study was designed to determine whether serum ANCA expression in patients with CD characterizes an identifiable clinical subgroup. METHODS: The study population consisted of 69 consecutive patients with an established diagnosis of CD as determined by a combination of characteristic clinical, radiographic, endoscopic, and histopathologic criteria. Sera from the patients were analyzed for the presence of ANCAs using the fixed neutrophil enzyme-linked immunosorbent assay (ELISA) assay. Perinuclear ANCA (pANCA)-positive and cytoplasmic ANCA (cANCA)-positive results by ELISA were confirmed by indirect immunofluorescence staining. Clinical profiles of the ANCA-positive patients with CD were compared with those of patients with CD not expressing ANCA (ANCA-negative). RESULTS: pANCA-positive patients with CD have endoscopically and/or histopathologically documented left-sided colitis and symptoms of left-sided colonic inflammation, clinically reflected by rectal bleeding and mucus discharge, urgency, and treatment with topical agents. One hundred percent of patients with CD expressing pANCA had "UC-like" features. CONCLUSIONS: In patients with CD, serum pANCA expression characterizes a UC-like clinical phenotype. Stratification of CD by serum pANCA provides evidence of heterogeneity within CD and suggests a common intestinal mucosal inflammatory process among a definable subgroup of patients with CD and UC expressing this marker.     

Exploring indicators for quantifying surface urban heat islands of European cities with MODIS land surface temperatures

The term urban heat island describes the phenomenon of altered temperatures in urban areas compared to their rural hinterlands. A surface urban heat island encompasses the patterns of land surface temperatures in urban areas. The classical indicator to describe a surface urban heat island is the difference between urban and rural surface temperatures. However, several other indicators for this purpose have been suggested in the literature. In this study, we compared the eleven different indicators for quantifying surface urban heat islands that were most frequently used in recent publications on remote sensing-based urban heat island assessments. The dataset used here consists of 263 European cities with monthly mean temperatures from MODIS data products for July 2002, January 2003 and July 2003. We found that (i) the indicators individually reveal diurnal and seasonal patterns but show rather low correlations over time, and (ii) for single points in time, the different indicators show only weak correlations, although they are supposed to quantify the same phenomenon. Differentiating cities according to thermal climate zones increased the relationships between the indicators. Thus, we can identify temporal aspects and indicator selection as important factors determining the estimation of urban heat islands. We conclude that research should take into account the differences and instabilities of the indicators chosen for quantifying surface urban heat islands and should use several indicators in parallel for describing the surface urban heat island of a city.     

Solving Third Order Differential Equations with Maximal Symmetry Group

The largest group of Lie symmetries that a third-order ordinary differential equation (ode) may allow has seven parameters. Equations sharing this property belong to a single equivalence class with a canonical representative v ^′′′(u)=0. Due to this simple canonical form, any equation belonging to this equivalence class may be identified in terms of certain constraints for its coefficients. Furthermore a set of equations for the transformation functions to canonical form may be set up for which large classes of solutions may be determined algorithmically. Based on these steps a solution algorithm is described for any equation with this symmetry type which resembles a similar scheme for second order equations with projective symmetry group. Received March 9, 2000; revised June 8, 2000     

Fluid dynamics of a quantized vortex filament in a hole

The behavior of nucleated vortex loops and of remanent vortex filaments in idealized circular and slit-like orifices has been investigated by direct computation. It is found that such vortices can be stretched by the diverging flow on the exit side of the orifice. The energy needed to stretch the vortex is abstracted from the flow field and observed as dissipation. This occurs in the form of discrete phase-slip events in the case of nucleated vortex loops, and in the form of multiple-phase-slip cascades when a remanent vortex is involved.     

Analysis of temperature-dependent optical gain in GaN-InGaN quantum-well structures

The temperature dependent spectral gain in InGaN-GaN multiple quantum-well structures with 10% In content is investigated. Mode gain is measured in a temperature range between 239 K and 312 K using the Hakki-Paoli technique and compared to simulations. The simulation accounts for temperature-dependent polarization dephasing, and hence homogeneous broadening, in a rigorous fashion, without any fit parameter. It is found that the evolution of the gain spectrum with temperature at different drive currents can be modeled using a temperature-independent single value for inhomogeneous broadening. The resulting compositional fluctuations are compared to structural measurements.     

Adaptive topology optimization of elastoplastic structures

Material topology optimization is applied to determine the basic layout of a structure. The nonlinear structural response, e.g. buckling or plasticity, must be considered in order to generate a reliable design by structural optimization. In the present paper adaptive material topology optimization is extended to elastoplasticity. The objective of the design problem is to maximize the structural ductility which is defined by the integral of the strain energy over a given range of a prescribed displacement. The mass in the design space is prescribed. The design variables are the densities of the finite elements. The optimization problem is solved by a gradient based OC algorithm. An elastoplastic von Mises material with linear, isotropic work-hardening/softening for small strains is used. A geometrically adaptive optimization procedure is applied in order to avoid artificial stress singularities and to increase the numerical efficiency of the optimization process. The geometric parametrization of the design model is adapted during the optimization process. Elastoplastic structural analysis is outlined. An efficient algorithm is introduced to determine the gradient of the ductility with respect to the densities of the finite elements. The overall optimization procedure is presented and verified with design problems for plane stress conditions.     

Fractionation of Starch by Gel Filtration on Agarose Beads

A chromatographical system consisting of a Sephadex G 200 column and an agarose 2B or 4B column was used for fractionation of native starches. Fractionation of the amylose and amylopectin components was achieved, and the amylose part of starch was subfractionated according to its molecular weight. The molecular weight of amylopectin is beyond the fractionation limit of the agarose and seems to be higher than 20 × 10⁶.     

Planar multimode detector arrays for infrared and millimeter-wave applications

A new type of detector array is described. By means of a suitably designed metallic network, many detector elements (each individually small compared to wavelength) are assembled into an impedance-matched termination for radiation incident normally on the plane of the device. Residual reactance is tuned out by means of a movable backshort. An array of 400 bismuth-film microbolometers with a total area of 1 cm²has been tested at 215 GHz. A coupling efficiency of approximately 60 percent was observed. The detector has aD*of4 times 10^{8}cm . Hz^1/2/W at room temperature with response time on the order of2 times 10^{-7}s. Similar arrays of Schottky and SIS diodes can probably be constructed.     

LXRα Regulates oxLDL-Induced Trained Immunity in Macrophages

Reprogramming of metabolic pathways in monocytes and macrophages can induce a proatherosclerotic inflammatory memory called trained innate immunity. Here, we have analyzed the role of the Liver X receptor (LXR), a crucial regulator of metabolism and inflammation, in oxidized low-density lipoprotein (oxLDL)-induced trained innate immunity. Human monocytes were incubated with LXR agonists, antagonists, and oxLDL for 24 h. After five days of resting time, cells were restimulated with the TLR-2 agonist Pam3cys. OxLDL priming induced the expression of LXRα but not LXRβ. Pharmacologic LXR activation was enhanced, while LXR inhibition prevented the oxLDL-induced inflammatory response. Furthermore, LXR inhibition blocked the metabolic changes necessary for epigenetic reprogramming associated with trained immunity. In fact, enrichment of activating histone marks at the IL-6 and TNFα promotor was reduced following LXR inhibition. Based on the differential expression of the LXR isoforms, we inhibited LXRα and LXRβ genes using siRNA in THP1 cells. As expected, siRNA-mediated knock-down of LXRα blocked the oxLDL-induced inflammatory response, while knock-down of LXRβ had no effect. We demonstrate a specific and novel role of the LXRα isoform in the regulation of oxLDL-induced trained immunity. Our data reveal important aspects of LXR signaling in innate immunity with relevance to atherosclerosis formation.