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991.
RT Gandhi BK Chen SE Straus JK Dale MJ Lenardo D Baltimore 《Canadian Metallurgical Quarterly》1998,187(7):1113-1122
The mechanism by which HIV-1 induces CD4(+) T cell death is not known. A fundamental issue is whether HIV-1 primarily induces direct killing of infected cells or indirectly causes death of uninfected bystander cells. This question was studied using a reporter virus system in which infected cells are marked with the cell surface protein placental alkaline phosphatase (PLAP). Infection by HIV-PLAP of peripheral blood mononuclear cells (PBMCs) and T cell lines leads to rapid depletion of CD4(+) T cells and induction of apoptosis. The great majority of HIV-induced T cell death in vitro involves direct loss of infected cells rather than indirect effects on uninfected bystander cells. Because of its proposed role in HIV-induced cell death, we also examined the Fas (CD95/Apo1) pathway in killing of T cells by HIV-1. Infected PBMCs or CEM cells display no increase in surface Fas relative to uninfected cells. In addition, HIV-1 kills CEM and Jurkat T cells in the presence of a caspase inhibitor that completely blocks Fas-mediated apoptosis. HIV-1 also depletes CD4+ T cells in PBMCs from patients who have a genetically defective Fas pathway. These results suggest that HIV-1 induces direct apoptosis of infected cells and kills T cells by a Fas-independent mechanism. 相似文献
992.
993.
YH An GW Stuart SJ McDowell SE McDaniel Q Kang RJ Friedman 《Canadian Metallurgical Quarterly》1996,14(5):846-849
Titanium surfaces were coated with bovine serum albumin using carbodiimide, a crosslinking agent. The durability of the coated surfaces and the inhibitory effect of the albumin coating on bacterial adherence were tested in vitro for 20 consecutive days at 37 degrees C in phosphate buffered saline, with intermittent agitation. The results showed that only 10% of the coated bovine serum albumin decayed off the surface during the 20-day incubation period. The inhibition rate of the albumin coating on bacterial adherence remained high (greater than 8.5%) throughout the experiment. The results suggested potential use of this crosslinked albumin coating to reduce bacterial adherence and thus the subsequent possibility of prosthetic or implant infection in vivo. 相似文献
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996.
F. Schwarz und . Weber 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1908,15(3):147-148
Ohne ZusammenfassungMitteilung aus dem Chemischen Untersuchungsamte der Stadt Hannover. 相似文献
997.
KS Hong SP Kim DL Na JG Kim YL Suh SE Kim JW Kim 《Canadian Metallurgical Quarterly》1997,42(12):1172-1176
Distribution of apolipoprotein(apo) E4 and E3 in lipoproteins of serum with apoE4/E3 phenotype was analyzed. ApoE was eluted in two major peaks by gel chromatography; peak 1 and 2 corresponding to very- and intermediate-low density lipoprotein (VLDL + IDL) and high density lipoprotein2 (HDL2), respectively. ApoE in peak 1 (VLDL + IDL) consisted of monomers of 34 kDa, complexes with a high molecular weight (apoEs) of 100 kDa and with a small amount of apoE-AII complexes weighing 43 kDa. In contrast, apoE in peak 2 (HDL2) was composed mainly of apoE-AII complexes and apoEs complexes, and a small amount of monomers. Both apoE3 and E4 isoforms were detected in these peaks; E4 was more predominant in peak 1 while E3 was more predominant in peak 2. These findings suggest that different distributions of apoE3 and E4 in lipoprotein particles. 相似文献
998.
VP Oleshko RH Gijbels AJ Van Daele WA Jacob YE Xu SE Wang IY Park TS Kang 《Canadian Metallurgical Quarterly》1998,42(2):108-122
The combination of cryo-energy filtering transmission electron microscopy (EFTEM)/electron spectroscopic diffraction (ESD)/electron energy-loss spectroscopy (EELS) and cryo-energy-dispersive X-ray (EDX) analysis in the scanning transmission (STEM) and scanning (SEM) modes was applied for the characterization of composite tabular Ag(Br,I) microcrystals. A low-loss fine structure in EEL spectra between 4 and 26 eV was attributed to excitons and plasmons possibly superimposed with interband transitions and many-electron effects. The contrast tuning under the energy-filtering in the low-loss region was used to image the crystal morphology, defect structure (random dislocations and ?111? stacking faults) and bend and edge contours as well as electron excitations in the microcrystals. Sharp extra reflections at commensurate positions in between the main Bragg reflections and diffuse honeycomb contours in ESD patterns of the microcrystals taken near the [111] zone were assigned to the number of defects in the shell region parallel to the grain edges and polyhedral clusters of interstitial silver cations, respectively. The imaginary part of the energy-loss function, Im (-1/epsilon), and the real and imaginary parts, epsilon1 and epsilon2, of the dielectric permittivity were determined by means of a Kramers-Kronig analysis. An assignment of exciton peaks based on calculations of electronic band structure of silver bromide is proposed. Inner-shell excitation bands of silver halide were detected in line with EDX-analyses. The energy-loss near-edge structure (ELNES) of the AgM4,5-edge governed by spin-orbital splitting between the 3d3/2- and 3d5/2-states has been evaluated. Combined silver and halide distributions were obtained by a three-window method (EFTEM) and by EDX/STEM including area mapping and line profiling of iodide. 相似文献
999.
Improved BGP convergence via ghost flushing 总被引:6,自引:0,他引:6
Afek Y. Bremler-Barr A. Schwarz S. 《Selected Areas in Communications, IEEE Journal on》2004,22(10):1933-1948
Labovitz et al. (2001) and Labovitz et al. (2000) noticed that sometimes it takes border gateway protocol (BGP) a substantial amount of time and messages to converge and stabilize following the failure of some node in the Internet. In this paper, we suggest a minor modification to BGP that eliminates the problem pointed out and substantially reduces the convergence time and communication complexity of BGP. Roughly speaking, our modification ensures that bad news (the failure of a node/edge) propagate fast, while good news (the establishment of a new path to a destination) propagate somewhat slower. This is achieved in BGP by allowing withdrawal messages to propagate with no delay as fast as the network forward them, while announcements propagate as they do in BGP with a delay at each node of one minRouteAdver (except for the first wave of announcements). As a by product of this work, a new stateless mechanism to overcome the counting to infinity problem is provided, which compares favorably with other known stateless mechanisms (in RIP and IGRP). 相似文献
1000.