Efficacy of organophosphorus derivatives containing chalcones/chalcone semicarbazones against fungal pathogens of sugarcane

Ten newly synthesized organophosphorus derivatives containing substituted chalcones and substituted chalcone semicarbazones were tested for their antifungal efficacy against Colletotrichum falcatum, Fusarium oxysporum, Curvularia pallescens (all sugarcane pathogens). The O,O-diethylphosphate derivatives containing 2-chlorochalcone and 2-chlorochalcone semicarbazone exhibited 70-85% mycelial inhibition against all the test fungi at 1000 ppm. The screening results were correlated with structural features of the tested compounds.     

Spatial-frequency-tuned mechanisms of the red-green channel estimated by oblique masking

The sustained spatial-frequency-tuned (SF-tuned) mechanisms of nonoriented units were examined by means of orthogonal masking for the Red-Green (R-G) color channel, and those of oriented units by oblique masking for the achromatic channel but not for the color channels. An oblique-masking technique minimizes the artifacts that are due to spatial phase effects, local cues, spatial beats, spatial probability summation, and changing criteria. Therefore the spatial characteristics of the R-G color channel are now investigated by an oblique-masking technique and linked with my paper on orthogonal masking [J. Opt. Soc. Am. A 15, 1 (1998)]. The R-G channel was defined by the minimum-flicker and hue-cancellation techniques. A color monitor system was used to generate spatially localized (D6) vertical color test patterns [0.063-8 cycles per degree (cpd)] and sinusoidal oblique color masks (0.031-16 cpd, 1.2-60% contrasts). Color contrast sensitivity functions (CSFs), threshold elevation (TE) versus mask SF (TvSF) curves, and TE versus mask contrast (TvC) curves were measured by the method of constant stimuli with a two-interval forced-choice technique by using Powell's achromatizing lens under sustained (Gaussian, 2-s-duration) conditions. Results show the following: (1) The color CSF is a low-pass function of SF with average half-height SF of 0.7 cpd and cutoff SF of 14 cpd with the use of a color-detection criterion. (2) TvSF curves are broadly bandpass and fall into five groups, peaking at approximately 0.13, 0.5, 2, 4, and 8 cpd. The root-mean-square cone-color CSF is 3.8-5.4 times the stimulus-color CSF. (3) A "crowding effect" similar to that of the TvSF curves of the achromatic channel was also found, but the TvSF curves of the R-G channel are not sharply peaked, similar to the result for orthogonal masking. Data analysis led to the following conclusions: (1) A simple multiple-mechanism model yields one low-pass color mechanism (with average half-height SF of 0.54 cpd) and five bandpass SF-tuned color mechanisms; these six mechanisms are necessary to explain the CSF, TvSF, and TvC data simultaneously. (2) The bandpass mechanisms peaked at approximately 0.13, 0.5, 2, 4, and 8 cpd with average full bandwidths at half-heights of 3.6, 3.2, 2.1, 1.2, and 1.3 octaves, respectively. (3) Since oblique-masking color mechanisms (unlike achromatic oriented mechanisms) have broad orientation tuning under sustained conditions and there is a significant orthogonal masking, the oblique-masking color mechanisms may have contributions from both oriented and nonoriented units. (4) The high degree of similarity between the SF-tuned filters of mechanisms derived from oblique- and orthogonal-masking data suggests that most of the chromatic SF tuning is already accomplished by nonoriented units. (5) The quality of the fit to oblique- and orthogonal-masking data combined dropped enough to reject the hypothesis that the former taps the performance of only the same nonoriented mechanisms as those by the latter. Adding gain parameters that reduce the TEs for orthogonal masking gave a better fit, suggesting that orientation gains are one of the factors involved in the transformation of information from nonoriented to oriented mechanisms. However, the fit was still worse than that for oblique-(6) Since masking-alone or orthogonal-masking-alone data, suggesting that more factors may be involved. primate parvo lateral geniculate nucleus (pLGN) units behave in a fairly linear manner, the color contrast nonlinearity (which follows the linear filter) of a mechanism may be post-pLGN.     

Damage-Coupled Progressive Failure and Yield Line Analyses of Metal Plates

Continuum damage mechanics based progressive failure analysis of an aluminum alloy AL2024-T3 plate has been carried out. Isotropic continuum damage mechanics model proposed by Chandrakanth and Pandey in 1995 has been implemented in a nonlinear finite element computational scheme based on damage-coupled and damage-uncoupled elastoplastic constitutive relationship. In order to model the progressive growth of damage and plasticity from extreme fibers toward the neutral axis, discrete layered approach has been adopted in the formulation using Ahmed’s degenerate isoparametric shell element, which accounts for shear deformation. A critical damage criteria is used for determining the onset and propagation of failure in the plate. Damage-coupled and damage-uncoupled analyses have been carried out on rectangular and triangular plates of aluminum alloy Al2024-T3. Yield line patterns have been generated using extensive nonlinear progressive failure analysis and comparison with conventional yield line analysis has been made. It is envisioned that employing the methodology presented herein, yield line pattern generation for structural components with complex shapes can be obtained, which would significantly assist engineers in analysis and design of structures.     

Inhibition of salvinia (Salvinia molesta Mitchell) by parthenium (Parthenium hysterophorus L.). I. Effect of leaf residue and allelochemicals

Parthenium (Parthenium hysterophorus L.) leaf residue (LP, leaf powder) inhibited salvinia (Salvinia molesta Mitchell) biomass and the number of healthy fronds at 0.25% (w/v) and killed the treated plants at and above 0.75% (w/v) in about 5–15 days, depending on the quantity of the residue. At the lethal dose, the LP caused an abrupt desiccation of above-water plant parts, probably due mainly to root dysfunction. This was concurrent with the loss of dehydrogenase activity in, and an increase in solute leakage from, the roots and loss of chlorophylla, b, and total chlorophyll contents in the fronds, resulting in death of the treated plants. The LP appears inhibitory to salvinia through affecting macromolecules—proteins, lipids, and nucleic acids. The inhibitory activity of LP at the lethal dose suspended in water was completely lost when allowed to stand for 30 days under outdoor conditions and promoted growth of the salvinia plants placed in it. The standard allelochemicals, including those present in parthenium LP, except parthenin andp-hydroxybenzoic acid, did not inhibit growth up to 100 ppm. However, parthenin andp-hydroxybenzoic acid killed salvinia plants at 100 and 50 ppm, respectively. Sincep-hydroxybenzoic acid is unlikely to be present at such a high concentration, parthenin appears to be one of the main allelochemicals responsible for the inhibitory effect of parthenium leaf residue on salvinia.A portion of this work was presented at the International Symposium on Weed Management for Sustainable Agriculture held at C.C.S. Haryana Agricultural University, Hissar, India, November 18–20, 1993.     

Relative merits of ultrasonography, computed tomography and cholangiography in patients of surgical obstructive jaundice

Fifty consecutive patients with surgical obstructive jaundice were evaluated prospectively with ultrasonography (US), computed tomographic scans (CT scan) and cholangiography-percutaneous transhepatic cholangiography (PTC) or endoscopic retrograde cholangio-pancreaticography (ERCP). The diagnostic accuracy of ultrasound in defining the level of obstruction was 86% as compared to 86% and 94.8% for CT scan and cholangiography, respectively. To measure the etiology of the obstruction, the accuracy of ultrasound, CT scan and cholangiography were 84%, 86% and 75%, respectively. The sensitivity of CT scans and cholangiography in the diagnosis of choledocholithiasis was 100%, 81.8% and 90%, respectively, whereas specificity was 97%, 100% and 100%, respectively. Sensitivity for a diagnosis of malignant disease was 100% for both US and CT scans whereas specificity was 90% and 81%, respectively. Ultrasonography as a single radiological investigation is sufficient in the evaluation of the majority of patients with surgical obstructive jaundice. CT scan and cholangiography should be done only when US gives equivocal findings or if concomitant therapeutic procedures like basketing and stenting are also planned.     

The effects of deprenyl on methamphetamine-induced dopamine depletions

The effects of deprenyl on methamphetamine-induced dopamine depletions were studied in mice. Four SC injections of 12.5 mg/kg of methamphetamine at two-hour intervals caused substantial (72-82%) and long-lasting depletions of striatal dopamine. Pretreatment with either 25 or 40 mg/kg of deprenyl did not significantly alter the magnitude of this depletion. These results indicate that, unlike what is observed following MPTP, there is no protection afforded dopaminergic cells by deprenyl pretreatment in the methamphetamine model of parkinsonism.     

In vitro expression and inhibition of procoagulant activity produced by bovine alveolar macrophages and peripheral blood cells

Local and systemic activation of coagulation is frequently associated with bacterial sepsis. The coagulopathy is due, at least in part, to expression of tissue factor (TF) by monocytes and macrophages. The purpose of this study was to evaluate the expression of procoagulant activity by bovine alveolar macrophages, leukocytes and platelets, and to determine the relative potency of three chemical inhibitors of TF expression (pentoxifylline, retinoic acid, and cyclosporin A). Bovine alveolar macrophages were stimulated with lipopolysaccharide (LPS) derived from Pasteurella haemolytica or recombinant bovine tumour nervous factor (TNF) and dose- and time-dependent effects on TF expression were studied. LPS and TNF induced TF expression in alveolar macrophages and LPS treatment of whole blood induced TF expression in mononuclear cells. Neutrophils and platelets also expressed procoagulant activity, but this activity was not inhibited by anti-bovine TF monoclonal antibody. Pentoxifylline (40 mumol/L), retinoic acid (0.01 mmol/L) and cyclosporin A (0.08 mumol/L) inhibited TF expression when added concurrently with LPS or TNF, but not when added 4 h after stimulation. TF mRNA was not detected in unstimulated alveolar macrophages by Northern blot analysis. In contrast, exposure to LPS or TNF for 6 h induced marked expression of TF mRNA, which was inhibited by treatment with pentoxifylline, retinoic acid and cyclosporin A. Expression of TNF by alveolar macrophages stimulated with LPS was also inhibited by these compounds. Our results indicate that procoagulant activity expressed by alveolar macrophages and monocytes is associated with expression of TF, whereas procoagulant activity expressed by neutrophils and platelets is not. The concentrations of pentoxifylline and retinoic acid necessary for inhibition of TF expression in vitro may not be achievable in vivo owing to their toxic effects. However, the in vitro concentration of cyclosporin A that inhibited TF expression did not exceed the plasma concentration observed in humans, and therefore may be useful for inhibition of TF expression in vivo.     

The Arabidopsis thaliana gametophytic mutation gemini pollen1 disrupts microspore polarity, division asymmetry and pollen cell fate

Pollen development and male gametogenesis are critically dependent upon cell polarization leading to a highly asymmetric cell division termed pollen mitosis I. A mutational approach was adopted in Arabidopsis thaliana to identify genes involved these processes. Four independent gemini pollen mutants were isolated which produce divided or twin-celled pollen. The gemini pollen1 mutant was characterized in detail and shown to act gametophytically resulting in reduced transmission through both sexes. gemini pollen1 showed an incompletely penetrant phenotype resulting in equal, unequal and partial divisions at pollen mitosis I. The division planes in gemini pollen1 were shown to be aligned with the polar axis (as in wild type) and evidence was obtained for incomplete nuclear migration, which could account for altered division symmetry. gemini pollen1 also showed division phenotypes consistent with spatial uncoupling of karyokinesis and cytokinesis suggesting that GEMINI POLLEN1 may be required for the localization of phragmoplast activity. Cell fate studies showed that in both equal and unequal divisions a vegetative cell marker gene was activated in both daughter cells. Daughter cells with a range of intermediate or hybrid vegetative/generative cell fates suggests that cell fate is quantitatively related to cell size. The potential mode of action of GEMINI POLLEN1 and its effects on cell fate are discussed in relation to proposed models of microspore polarity and cell fate determination.     

Diffusion of Impurity Excitations in Superfluid and Solid 3He–4He Mixtures

Starting from the system of linearized kinetic equations, the exact expressions for the spin and mass diffusion coefficients and thermal diffusion ratio in quantum mixtures of helium isotopes with any degree of degeneration is obtained. From the general expression the various limiting expressions have been obtained, which are determined by various relations between collision rates of quasiparticles. The results of the calculations are compared with available experimental data. It is shown that in the low-temperature region, where an impuriton gas is one-component, the relaxation of concentration of ³ He in superfluid ³ He– ⁴ He mixtures is determined by the acoustic and dissipative collective modes with an effective diffusion coefficient. From the general relation, the expression for a spin diffusion coefficient in solid ³ He– ⁴ He quantum mixtures is derived. The comparison of the results obtained with the experimental data makes it possible to find a numerical value for the width of the impuriton energy zone.