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31.
DJ Benos BH Hahn JK Bubien SK Ghosh NA Mashburn MA Chaikin GM Shaw EN Benveniste 《Canadian Metallurgical Quarterly》1994,91(2):494-498
Infection by human immunodeficiency virus type 1 (HIV-1) is often complicated by a variety of neurological abnormalities. The most common clinical syndrome, termed acquired immunodeficiency syndrome (AIDS) dementia complex, presents as a subcortical dementia with cognitive, motor, and behavioral disturbances and is unique to HIV-1 infection. The pathogenesis of this syndrome is poorly understood but is believed to involve interactions among virally infected macrophages/microglia, astrocytes, and neurons. In this study, we show that exposure of primary rat and human astrocytes to heat-activated HIV-1 virions, or to eukaryotically expressed HIV-1 and HIV-2 envelope glycoproteins (gp120) stimulates amiloride-sensitive Na+/H+ antiport, potassium conductance, and glutamate efflux. These effects are blocked specifically by amiloride, an inhibitor of Na+/H+ antiport and by the selective removal of gp120 with immobilized monoclonal antibody. As a result of modulation of astrocytic function by gp120, the ensuing neuronal depolarization and glutamate exposure could activate both voltage-gated and N-methyl-D-aspartate-regulated Ca2+ channels, leading to increases in intraneuronal Ca2+ and neuronal death. These findings implicate the astrocyte directly in the pathogenesis of AIDS dementia complex. 相似文献
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A mild thermal treatment of wood leads to improved macroscopic properties (dimensional stabilization and resistance against fungal degradation). The chemical modifications induced by the thermal treatment were investigated by means of DRIFT spectroscopy on wood blocks in order to explain the new macroscopic properties on a molecular level. The formation of new ether linkage was observed in addition to the well-known acidic hydrolysis reactions of wood. Fungal attack was found to take place even after thermal degradation of pentosanes by a standard oxidative way. A competition between depolymerization and condensation reactions was observed. 相似文献
36.
Hybrid finite-element method for discretising cylindrically symmetric parts in electrotechnical models 总被引:1,自引:0,他引:1
In many technical devices such as transformers and electrical machines, large differences in geometric dimensions are observed. As a consequence, the generation of a 3D computational grid for the whole device leads to unacceptably large numbers of elements or can even fail. In addition to the commonly applied cartesian or cylindrical symmetries of the overall geometry, the model can be subdivided into parts featuring translational or cylindrical symmetries. Such parts are discretised separately, accounting for the local symmetry, and are then combined with the surrounding 3D model. Excitations and boundary conditions of the submodels are not necessarily symmetric but are expected to be smooth in the direction of the symmetry. Then, the field distribution at the interface is well approximated by a set of spectral elements along the dimension of symmetry. Coupling between the model parts is carried out by means of Lagrange multipliers. A single-phase transformer with thin insulation sheets is taken as an example to illustrate the proposed hybrid discretisation. The cross-section of the cylindrically symmetric part containing thin sheets, is represented by a fine 2D finite-element mesh so that all the geometrical details can be resolved, and the rest of the structure is discretised by a 3D mesh. Nevertheless, a fully 3D field distribution is calculated in all model parts. Only a small number of harmonic functions is needed to account for the azimuthal field variation at the cylindrical interface. Hence, the number of unknowns in the numerical model is reduced significantly, while a high level of accuracy is maintained 相似文献
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We have compared light chain immunohistochemistry in reactive lymphoid tissue and a series of paraffin-embedded B-cell lymphomas using standard trypsin digestion with a heat mediated epitope retrieval method. Fifty-seven B-cell lymphomas (18 high grade, 29 low grade and 10 cases of nodular lymphocyte predominance Hodgkin's disease), two reactive lymph nodes and eight tonsils fixed for known times between 12 h and 2 years were studied. Paraffin-embedded tissue was stained with polyclonal anti-kappa and anti-lambda antibodies. For each antibody staining was performed on two sections, one treated with trypsin digestion and one with microwave heating. Sections were scored from 0 to + + + with 0 representing poor staining and + + + excellent staining. A score of ++ was considered satisfactory. Light chain restriction was recorded if present. Satisfactory staining was obtained in 34/59 cases using trypsin digestion and 56/59 cases using heat retrieval. Light chain restriction was demonstrated in 32/57 (56%) B-cell lymphomas using trypsin digestion and 52/57 (91%) using heat retrieval. Satisfactory staining was obtained in tonsils fixed for up to 48 h using trypsin digestion and up to 2 years using heat retrieval. We have shown that for light chain immunostaining a heat mediated epitope retrieval method produces more consistent and satisfactory results and is effective over a greater range of fixation times than traditional trypsin digestion. 相似文献
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Polypropylene mesh, the routinely used material for mesh laparostomies is expensive. A economical and easily available substitute-nylon net was compared with polypropylene mesh with regard to efficacy and wound complications in a retrospective study. 51 patients, 25 in the polypropylene mesh group and 26 in the nylon net group were studied. The groups were matched with respect to age, sex, indication for surgery and mortality. The mean hospital stay (65 vs 54 days), fate of the wound, incidence of mesh extrusion (26% vs 20%), bowel perforation (1 vs none) and incisional hernia formation (21% vs 20%) in those with polypropylene mesh and nylon net respectively was similar. In conclusion, nylon net is an effective and inexpensive indigenous substitute for polypropylene mesh for mesh laparostomies. 相似文献
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