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121.
Richmond VL Rayson MP Wilkinson DM Carter JM Blacker SD Nevill A Ross JD Moore S 《Ergonomics》2008,51(6):935-946
Since 2002, the Royal Air Force (RAF) has been working towards developing role-related physical tests for use as an operational fitness test (OFT). The purpose of this study was to establish reliability of the OFT (comprising four tests), investigate gym-based tests as predictors of performance and establish performance standards. Fifty-eight RAF personnel performed the OFT on three occasions. A separate cohort carried out fitness and anthropometric tests before performing the OFT, by way of establishing performance predictors. Documented evidence and views of an expert panel were used to determine OFT standards. Reliability ranged from moderate to good for three tests, with one test (Dig) showing poor reliability. The 95% limits of agreement for the prediction models ranged from good to poor (6.7-34.2%). The prediction models were not sufficiently accurate to estimate confidently OFT performance, but could be used as a guide to quantify likely outcome and training needs. 相似文献
122.
This study investigated the physiological responses and limitations to a simulated search and rescue scenario in a high-rise building under ambient conditions. Sixteen firefighters performed the scenario under four conditions: standard duration breathing apparatus (SDBA) and 45 mm hose; extended duration breathing apparatus (EDBA) and 45 mm hose; SDBA and 70 mm hose; EDBA and 70 mm hose. Core temperature, skin temperature and heart rate were monitored. In four of 32 trials the casualty was rescued; the remainder of the trials were terminated for safety, high core temperature or shortage of air. Final core temperature and heart rate were higher in the EDBA (39.1 degrees C; 72% heart rate reserve (HRR)) than SDBA conditions (38.6 degrees C; 67%HRR). No differences were observed between hose sizes. The scenario proved too onerous to complete successfully in the majority of cases. Replacing SDBA with EDBA eliminates air supply as a limiting factor, but brings with it challenges of managing thermal strain. 相似文献
123.
A new scanning airborne-aerosol lidar system that has the potential to be a valuable atmospheric remote-sensing tool has been developed. The system has the ability to scan both parallel and perpendicular to an aircraft's flight path, and this ability permits both the three-dimensional rendering of the aerosol structure below the aircraft and the measurement of aerosol extinction and optical depth. The system has been integrated into a NASA P-3 aircraft and during a recent flight was used to acquire excellent data with both scanning modes. The system design, the application of the across-track scanning data to the study of the atmospheric boundary layer, and the computation of optical depth derived from along-track scan data are reported. 相似文献
124.
A computational model was developed to explain the effects of an interframe interval (IFI) in single-step apparent motion experiments. In these experiments a stimulus appears in one position, disappears, and then reappears in a shifted position after a short or long IFI. If the luminance during the IFI matches the mean luminance of the stimulus frames, long IFIs result in perceived motion opposite the short-IFI conditions. Brighter or darker IFIs, however, do not support the reversed motion effect. The model possess the following defining characteristics: (1) a biphasic ("transient") channel whose signalled direction of motion reverses with changes of IFI duration; (2) a combined direction-opponent output which is the sum of directional responses developed in two channels--biphasic ("transient") and monophasic ("sustained"); (3) a signal/noise weighting of the contributions of the two channels to the final directional output of the system. Predictions of the model about the effects of IFI intensity and viewing eccentricity were tested and confirmed in two new psychophysical experiments. The interpretations of past studies which included a role for second-order motion mechanisms in explaining IFI duration effects were reexamined. Further empirical tests of the model were outlined. 相似文献
125.
Engineering linear F(ab')2 fragments for efficient production in Escherichia coli and enhanced antiproliferative activity 总被引:1,自引:0,他引:1
Zapata Gerardo; Ridgway John B. B.; Mordenti Joyce; Osaka Gary; Wong Wai Lee T.; Bennett Gregory L.; Carter Paul 《Protein engineering, design & selection : PEDS》1995,8(10):1057-1062
We developed a novel bivalent antibody fragment, the linear(L-) F(ab')2, comprising tandem repeats of a heavy chain fragmentVHCH1VHCH1 cosecreted with a light chain.Functional humanized L-F(ab')2 directed against p185HER2 wassecreted from Escherichia coli at high titer (100 mg/l) andpurified to homogeneity. The L-F(ab')2 binds two equivalentsof antigen with an apparent affinity (Kd = 0.46 nM) that iswithin 3-fold of the corresponding thioether-linked F(ab')2fragment The N-terminal site binds antigen with an affinity(Kd = 1.2 nM) that is 4-fold greater than that for the C-terminalsite, as shown by the comparison of L-F(ab')2 variants containinga single functional binding site. L-F(ab')2 has greater antiproliferativeactivity than the thioether-linked F(ab')2 against the p185HER2-overexpressingtumor cell line BT474. Linear and thioether-linked F(ab')2 havevery similar pharmacokinetic properties in normal mice, andtheir serum permanence times are respectively 7- and 8-foldlonger than the corresponding Fab fragment L-F(ab')2 offersa facile route to bivalent antibody fragments that are potentiallysuitable for clinical applications, and that may have improvedbiological activity compared with thioether-linked F(ab')2 fragments. 相似文献
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MXene Yarn Supercapacitors: High‐Performance Biscrolled MXene/Carbon Nanotube Yarn Supercapacitors (Small 37/2018) 下载免费PDF全文
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RasGRP, a Ras guanyl nucleotide- releasing protein with calcium- and diacylglycerol-binding motifs 总被引:1,自引:0,他引:1
JO Ebinu DA Bottorff EY Chan SL Stang RJ Dunn JC Stone 《Canadian Metallurgical Quarterly》1998,280(5366):1082-1086
RasGRP, a guanyl nucleotide-releasing protein for the small guanosine triphosphatase Ras, was characterized. Besides the catalytic domain, RasGRP has an atypical pair of "EF hands" that bind calcium and a diacylglycerol (DAG)-binding domain. RasGRP activated Ras and caused transformation in fibroblasts. A DAG analog caused sustained activation of Ras-Erk signaling and changes in cell morphology. Signaling was associated with partitioning of RasGRP protein into the membrane fraction. Sustained ligand-induced signaling and membrane partitioning were absent when the DAG-binding domain was deleted. RasGRP is expressed in the nervous system, where it may couple changes in DAG and possibly calcium concentrations to Ras activation. 相似文献