Water-soluble copolymers: 22. Copolymers of acrylamide with 2-acrylamido-2-methylpropanedimethylammonium chloride: Aqueous solution properties of a polycation

The viscometric, turbidimetric and potentiometric properties of copolymers of acrylamide (AM) with 2-acrylamido-2-methylpropanedimethylammonium chloride (AMPDAC) were studied in aqueous solutions. The AMPDAC polymers exhibit poor salt tolerance and large, negative viscosity/temperature coefficients. Furthermore, the polymers were found to be sensitive to changes in pH. The AMPDAC polymers undergo phase separation in the presence of dianions as a function of temperature and AMPDAC composition.     

Role of IL-4 and IFN-gamma in modulation of immunity to Borrelia burgdorferi in mice

Because T cells appear to modulate the severity of murine Borrelia burgdorferi infections, we decided to examine the possible involvement of T cell-associated cytokines in disease outcome. Comparison of in vitro B. burgdorferi Ag-induced cytokine production in disease-susceptible and -resistant strains revealed striking differences; spleen cells from susceptible C3H mice produced significantly higher levels of IL-2 and IFN-gamma and lower levels of IL-4 than spleen cells from resistant BALB/c mice. Lymph node responses were even more divergent, with C3H mice producing high levels of IFN-gamma, and BALB/c mice producing little or none. This apparent Th1/Th2 cytokine imbalance was also reflected in vivo, since serum from C3H had significantly higher levels of B. burgdorferi-specific IgG2a Ab and lower levels of IgG1 Ab than serum from BALB/c mice. In vivo studies confirmed the importance of IL-4 in early control of spirochete growth, since treatment of either strain with neutralizing anti-IL-4 mAb led to increased joint swelling and higher spirochete burdens in joints compared with those in control mAb-treated mice. In contrast, IFN-gamma may hinder early control of spirochete growth in susceptible C3H mice, since treatment of mice with neutralizing anti-IFN-gamma mAb reduced both joint swelling and joint spirochete burdens compared with those in control mAb-treated mice. These studies indicate opposing roles for IL-4 and IFN-gamma in the modulation of spirochete growth and disease development in B. burgdorferi-infected mice and suggest that differential cytokine production early in infection may contribute to strain-related differences in susceptibility.     

Multiplexed biochemical assays with biological chips

High density peptide and oligonucleotide chips are fabricated using semiconductor-based technologies. These chips have a variety of biological applications.     

Simultaneous design of membership functions and rule sets for fuzzycontrollers using genetic algorithms

This paper examines the applicability of genetic algorithms (GA's) in the simultaneous design of membership functions and rule sets for fuzzy logic controllers. Previous work using genetic algorithms has focused on the development of rule sets or high performance membership functions; however, the interdependence between these two components suggests a simultaneous design procedure would be a more appropriate methodology. When GA's have been used to develop both, it has been done serially, e.g., design the membership functions and then use them in the design of the rule set. This, however, means that the membership functions were optimized for the initial rule set and not the rule set designed subsequently. GA's are fully capable of creating complete fuzzy controllers given the equations of motion of the system, eliminating the need for human input in the design loop. This new method has been applied to two problems, a cart controller and a truck controller. Beyond the development of these controllers, we also examine the design of a robust controller for the cart problem and its ability to overcome faulty rules