Effects of metal-contaminated soils on the growth, sexual development, and early cocoon production of the earthworm Eisenia fetida, with particular reference to zinc

Juvenile Eisenia fetida (Savigny) were exposed for 20 weeks to an uncontaminated soil and to soils contaminated with cadmium, copper, lead, and zinc collected from seven sites at different distances from a smelting works at Avonmouth, southwest England. The survival, growth (= weight after 5 weeks exposure), time to sexual maturation (= percentages of adults present after 8 weeks), and reproduction (= number of cocoons produced by the worms) were compared with soil metal concentrations. Of the parameters measured, growth and sexual maturation time had the lowest EC50 values. The effects of metal-contaminated soils could be attributed both to the direct toxicity of the metals and to changes in the "scope for growth" of the exposed worms. A comparison of the results with those of an earlier toxicity test conducted with adult worms indicated that juveniles are more sensitive to metals than adults. Significant toxic effects on the growth and sexual maturation times of juveniles were detected in soils from sites for which no significant effects on the cocoon production of adults could be detected. The greater sensitivity of juvenile worms indicates the importance of considering effects on a variety of life history stages when conducting a risk assessment of the effects of pollutants in soils. Although E. fetida does not occur naturally in soils at Avonmouth, the present study provides evidence to support the suggestion that pollution from the smelter is responsible for the absence of worms within 2 km of the factory. Results presented in this paper, and from previous studies, suggest the observed absence is due to the effects of zinc on the growth and maturation of juveniles and the cocoon production rate of adult worms.     

Development into blastocysts of bovine oocytes cryopreserved by ultra-rapid cooling

The objective of the research described was to devise an efficient procedure to cryopreserve in vitro-matured bovine oocytes, using in vitro fertilization (IVF) and development of resultant zygotes into blastocysts as criteria of oocyte survival. Oocytes at metaphase II were found to be extremely sensitive to chilling. Cooling them to O degrees C for as little as 5 sec significantly decreased their capability to cleave and develop further after IVF; after 80 sec at 0 degrees C, only approximately 10% of chilled oocytes developed into blastocysts. Oocytes were also adversely affected by brief exposures to 4 M and 5.5 M ethylene glycol (EG) solutions supplemented with sucrose; after being suspended in either of these EG solutions in plastic straws and plunged directly into liquid nitrogen (LN2), few of the oocytes were fertilized and developed. To "outrace" chilling injury, oocytes contained in < 1 microliter of EG solution were placed onto electron microscope grids and plunged directly into N2 slush or LN2. After such ultra-rapidly cooled oocytes were warmed, 30% of them cleaved after IVF, and half of these developed into blastocysts-- survival rates equivalent to those for oocytes that had been exposed to EG without any cooling. This method offers promise as a novel way to cryopreserve bovine oocytes.     

User-Sensitive Inclusive Design

Although “User-Centred”, “Participatory”, and other similar design approaches have proved to be very valuable for mainstream design, their principles are more difficult to apply successfully when the user group contains, or is composed of, older and/or disabled users. In the field of design for older and disabled people, the “Universal Design”, “Inclusive Design” and “Design for All” movements have encouraged designers to extend their design briefs to include older and disabled people. The downside of these approaches is that they can tend to encourage designers to follow a traditional design path to produce a prototype design, and only then investigate how to modify their interfaces and systems to cope with older and/or disabled users. This can lead to an inefficient design process and sometimes an inappropriate design, which may be “accessible” to people with disabilities, but in practice unusable. This paper reviews the concept that the authors have called “User-Sensitive Inclusive Design”, which suggests a different approach to designing for marginalised groups of people. Rather than suggesting that designers rely on standards and guidelines, it is suggested that designers need to develop a real empathy with their user groups. A number of ways to achieve this are recommended, including the use of ethnography and techniques derived from professional theatre both for requirements gathering and for improving designers’ empathy for marginalised groups of users, such as older and disabled people.     

Video game experience predicts virtual, but not real navigation performance

In recent years, studies have shown that video game experience is related to improvements across a variety of cognitive and visuospatial tasks. This study investigated the relationship between prior video game experience and spatial performance in virtual and real environments. Across two experiments, gaming experience was related to performance in desktop virtual environments; those with more video game experience were more accurate in pointing to nonvisible targets. In contrast, gaming experience was unrelated to three different real environment tasks, suggesting that video games may primarily influence perceptual and cognitive abilities in the visual domain over abilities that also involve kinesthetic or vestibular input. Contrary to expectations, gaming experience was also related to performance in immersive virtual environments, which may be related to the use of a joystick interface during immersive travel. Video game experience was also positively related to performance in a dynamic spatial task and to verbal SAT and math SAT scores. Sex differences in desktop virtual navigation and dynamic spatial ability were eliminated when game experience was included as a covariate.     

The Shadow Knows: Refinement and security in sequential programs

Stepwise refinement is a crucial conceptual tool for system development, encouraging program construction via a number of separate correctness-preserving stages which ideally can be understood in isolation. A crucial conceptual component of security is an adversary’s ignorance of concealed information. We suggest a novel method of combining these two ideas.Our suggestion is based on a mathematical definition of “ignorance-preserving” refinement that extends classical refinement by limiting an adversary’s access to concealed information: moving from specification to implementation should never increase that access. The novelty is the way we achieve this in the context of sequential programs.Specifically we give an operational model (and detailed justification for it), a basic sequential programming language and its operational semantics in that model, a “logic of ignorance” interpreted over the same model, then a program-logical semantics bringing those together — and finally we use the logic to establish, via refinement, the correctness of a real (though small) protocol: Rivest’s Oblivious Transfer. A previous report^? treated Chaum’s Dining Cryptographers similarly.In passing we solve the Refinement Paradox for sequential programs.     

Compositional noninterference from first principles

The recently formulated Shadow Semantics for noninterference-style security of sequential programs avoids the Refinement Paradox by preserving demonic nondeterminism in those cases where reducing it would compromise security. The construction (originally) of the semantic domain for The Shadow, and the interpretation of programs in it, relied heavily on intuition, guesswork and the advice of others. That being so, it is natural after the fact to try to reconstruct an idealised “inevitable” path from first principles to where we actually ended up: not only does one learn (more) about semantic principles by doing so, but the “rational reconstruction” helps to expose the choices made, along the way, and to legitimise the decisions that resolved them. Unlike our other papers on noninterference, this one does not contain a significant case study: instead its aim is to provide the most accessible account we can of the methods we use and why our model, in its details, has turned out the way it has. In passing, it might give some insight into the general role and significance of compositionality and testing-with-context for program semantics. Finally, a technical contribution here is a new “Transfer Principle” that captures uniformly a large class of classical refinements that remain valid when noninterference is taken into account in our style.     

Pain and dissociation in the cold pressor test: A study of hypnotic analgesia with "hidden reports" through automatic key pressing and automatic talking.

To test whether pain blocked by hypnotic analgesia may still be perceived at some level, 20 highly hypnotizable undergraduates participated in an experiment involving cold pressor pain in the normal condition and in hypnotically suggested analgesia. 3 reports were obtained reflecting felt pain within the hypnotic analgesia condition: the usual verbal report on a numerical scale, a manual report by "automatic key pressing," and a retrospective verbal report through "automatic talking." 9 Ss who were amnesic for both keypressing and automatic talking reported more pain in the automatic (hidden) reports than in their usual verbal reports. 8 of these 9, following release of amnesia, had a clear perception of 2 levels of awareness of the pain: the usual hypnotic experience of pain attenuated by analgesia suggestions, and a knowledge at another level of a more severe pain. In no case, however, did an S give a retrospective report of normal suffering at this "hidden" level. The hypnotically analgesic S may have reported no pain verbally because he was amnesic for it; when amnesia was removed he recalled the sensory pain, but without a suffering component, because suffering apparently did not occur. (24 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Efficient surface reconstruction from contours based on two‐dimensional Delaunay triangulation

This paper introduces an efficient method for surface reconstruction from sectional contours. The surface between neighbouring sections is reconstructed based on the consistent utilization of the two‐dimensional constrained Delaunay triangulation. The triangulation is used to extract the parametric domain and to solve the problems associated with correspondence, tiling and branching in a general framework. Natural distance interpolations are performed in order to complete the mapping of the added intermediate points. Surface smoothing and remeshing are conducted to optimize the initial surface triangulations. Several examples are presented to demonstrate the effectiveness and efficiency of the proposed approach. Copyright © 2005 John Wiley & Sons, Ltd.     

Remotely sensed indicators of habitat heterogeneity: Use of synthetic aperture radar in mapping vegetation structure and bird habitat

An integrated remote sensing/field ecology project linked the use of synthetic aperture radar (SAR) and aerial photography to studies of landscape spatial heterogeneity and bird community ecology. P-, L-, and C-band SAR data, collected over a section of Kakadu National Park in Australia's Northern Territory during the Joint NASA/Australia DC-8 data acquisition campaign, were analyzed in light of field data integrating vegetation structure and floristics with bird abundances across a heterogeneous study site. Results indicate that SAR data are able to discern structural differences relevant to bird habitat quality within floristically homogeneous stands, while multispectral sensors successfully identified floristic differences among habitat types. Simplifying indices of bird diversity showed ambiguous changes across the site; however, the abundances of individual species were observed to change significantly across both floristic and structural gradients. These results suggest that efforts to map bird diversity should focus on species-specific habitat relationships and that some measure of vegetation structure is needed to understand bird habitat. The approach employed here advances the use of SAR data in the three-dimensional mapping of animal habitats from remotely sensed data, and extends current capabilities for mapping and modeling large-scale patterns in the distribution of biological diversity.