A requirement for the rac1 GTPase in the signal transduction pathway leading to cardiac myocyte hypertrophy

We have used adenoviral-mediated gene transfer of a constitutively active (V12rac1) and dominant negative (N17rac1) isoform of rac1 to assess the role of this small GTPase in cardiac myocyte hypertrophy. Expression of V12rac1 in neonatal cardiac myocytes results in sarcomeric reorganization and an increase in cell size that is indistinguishable from ligand-stimulated hypertrophy. In addition, V12rac1 expression leads to an increase in atrial natriuretic peptide secretion. In contrast, expression of N17rac1, but not a truncated form of Raf-1, attenuated the morphological hypertrophy associated with phenylephrine stimulation. Consistent with the observed effects on morphology, expression of V12rac1 resulted in an increase in new protein synthesis, while N17rac1 expression inhibited phenylephrine-induced leucine incorporation. These results suggest rac1 is an essential element of the signaling pathway leading to cardiac myocyte hypertrophy.     

Preparation and characterization of bio-nanocomposite films based on soy protein isolate and montmorillonite using melt extrusion

The non-biodegradable and non-renewable nature of plastic packaging has led to a renewed interest in packaging materials based on bio-nanocomposites (biopolymer matrix reinforced with nanoparticles such as layered silicates). Bio-nanocomposite films based on soy protein isolate (SPI) and montmorillonite (MMT) were prepared using melt extrusion. Effects of the pH of film forming solution, MMT content, and extrusion processing parameters (screw speed and barrel temperature distribution) on the structure and properties of SPI–MMT bio-nanocomposite films were investigated. X-ray diffraction (XRD), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) were used for structural characterization of the films. Properties of the films were determined by tensile testing, dynamic mechanical analysis (DMA), thermogravimetric analysis (TGA), and water vapor barrier measurement. The arrangement of MMT in the soy protein matrix ranged from exfoliated at lower MMT content (5%) to intercalated at higher MMT content (15%). There was a significant improvement in mechanical (tensile strength and percent elongation at break) and dynamic mechanical properties (glass transition temperature and storage modulus), thermal stability, and water vapor permeability of the films with the addition of MMT. The results presented in this study show the feasibility of using bio-nanocomposite technology to improve the properties of biopolymer films based on SPI.     

Stabilization of highly concentrated suspensions of iron nanoparticles using shear-thinning gels of xanthan gum

Nanoscale zerovalent iron (NZVI) particles have recently become subject of great interest in the field of groundwater remediation for their ability to treat a wide variety of organic and inorganic contaminants. However, the field application of this technology is strongly hindered by the lack of stability of NZVI water suspensions.This study demonstrates that highly concentrated NZVI slurries (15 g/L) can be stabilized for more than 10 days adding 6 g/L of xanthan gum biopolymer. Stability against aggregation and sedimentation was achieved in the range of ionic strength 6 × 10⁻³–12 mM and is mainly due to the formation of a viscous gel characterized by shear-thinning behaviour.     

SML-Sys: a functional framework with multiple models of computation for modeling heterogeneous system

System-on-Chip and other complex distributed hardware/software systems contain heterogeneous components. High-level modeling of such systems require frameworks that provide designers with the ability to express concepts of models of computation (MoC)s as modeling constructs. Many system-level modeling frameworks and corresponding modeling notations such as Ptolemy II and SystemC-H facilitate multi-MoC modeling but are based on imperative programming languages (C++, Java, etc). In such frameworks, the computation and communication aspects between the components of models get intertwined thereby hindering its amenability to formal analysis. In this work, we illustrate function-based semantic definitions of MoCs, which are formulated in a functional framework called SML-Sys. We illustrate through a number of examples how to create system models using this functional programming paradigm.     

Mining metadata for composability of IPs from SystemC IP library

Exploring the design space when constructing a system is vital to realize a well performing design. Design complexity has made building high-level system models to explore the design space an essential but time-consuming and tedious part of the system design. Reduction in design time and acceleration of design exploration can be provided through reusing IP-cores to construct system models. As a result, it is common to have high-level SoC design flow based on IP libraries promoting reuse. However, the success of these would be dependent on how introspection and reflection capabilities are provided as well as what are the interoperability standard defined. This leads to the important question of what kind of IP metadata must be available to allow CAD tools to effectively manipulate these designs as well as allow for a seamless integration and exchange design information between tools and design flows. In this article, we describe our tools and methodology, which allow introspection of SystemC designs, such that the extracted metadata enables IP composition. We discuss the issues related to extraction of metadata from IPs specified in SystemC and show how our methodology combines C++ and XML parsers and data structures to achieve the above.     

Continuous Flow Microwave‐Assisted Processing and Aseptic Packaging of Purple‐Fleshed Sweetpotato Purees

ABSTRACT: Pumpable purees from purple‐flesh sweetpotatoes (PFSP) were subjected to microwave heating using a 60 kW, 915 MHz continuous flow system, followed by aseptic packaging in flexible containers to obtain a shelf‐stable product. Initial test runs were conducted using a 5 kW 915 MHz microwave system to measure dielectric in‐line properties and examine the puree temperature profiles. The results demonstrated uniformity in heating of the puree at sterilization temperatures (>121 °C), and the dielectric constants and loss factors were within the range of published values for orange‐fleshed sweetpotato purees. The pilot‐scale test runs in a 60 kW microwave unit produced shelf‐stable puree packages stable at room temperature. Polyphenolic content of the PFSP purees were evaluated and the results showed that while total phenolics increased (5.9%) and total monomeric anthocyanins slightly decreased (14.5%) with microwave application, antioxidant activity determined by 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging activity and oxygen radical absorbance capacity (ORAC) assays did not significantly change as a result of microwave processing. Color values showed that microwave‐processed samples differed from fresh puree in saturation and hue angle, but not in overall color change. PFSP purees increased in gel strength when microwave processed, packaged, and stored, but the gel could be easily disrupted into flowable purees. Overall, high‐quality retention can be obtained by microwave processing and aseptic packaging of PFSP purees so that they can be used as functional food ingredients.     

Sunflower Acid Oil-Based Production of Rhamnolipid Using Pseudomonas aeruginosa and Its Application in Liquid Detergents

Utilization of industrial waste as substrates for the rhamnolipid synthesis by Pseudomonas aeruginosa is a worthy alternative for conventionally used vegetable oils and fatty acids to reduce the production cost of rhamnolipid. Sunflower acid oil (SAO), a by-product of the oil industry, contains 70% 18:0 fatty acid, with oleic acid as a major component. In this scope, production and analysis of rhamnolipid was successfully demonstrated using SAO as a new substrate. Pseudomonas aeruginosa produced rhamnolipid (a glycolipid biosurfactant) at a maximum concentration of 4.9 g L⁻¹ with 60 g L⁻¹ of SAO in the medium. Structural properties of rhamnolipid biosurfactant are confirmed using thin layer chromatography (TLC), high performance liquid chromatography (HPLC), and fourier transformed infrared spectroscopy (FTIR) analysis. Further surface-active properties of the crude rhamnolipid were evaluated by measuring surface tension and emulsification properties. The synthesized rhamnolipid reduced the surface tension of water to 30.12 mN m⁻¹ and interfacial tension (against heptane) to 0.52 mN m⁻¹. Moreover, rhamnolipid shows the highest emulsification index (above 80%) for vegetable oils. This study confirms the use of SAO as a potential substrate for rhamnolipid production. The synthesized rhamnolipid was incorporated in liquid detergent formulation along with alpha olefin sulfonate (AOS) and sodium lauryl ether sulfate (SLES). The performance properties including foaming and cleaning efficiency of liquid detergent were compared.     

Developmental changes in storage proteins and peptidyl prolyl cis–trans isomerase activity in grains of different wheat cultivars

In the present study, storage proteins from five different wheat cultivars were extracted, fractionated and evaluated for their accumulation at different stages of development. SDS–PAGE analysis revealed that the accumulation of high molecular weight glutenin subunits was cultivar and stage dependent. However, low molecular weight glutenin subunits’ accumulation was not altered significantly after 16 days post anthesis in any of the cultivars. The rheological parameters (storage- and loss-modulus) of dough and gluten showed close association with either gliadins or glutenins. Peptidyl prolyl cis–trans isomerase (PPIase) activity, measured at different stages of grains development, showed variability with both the developmental stage and cultivar, and appeared to be primarily due to cyclophilins. Principal component analysis revealed the association of PPIase activity with either gliadin or total proteins, suggesting their significant role in the deposition of storage proteins in wheat.