The effect of colloid stability of wastewater treatment plants effluent on nanofiltration performance.

Laboratory-scale filtration tests utilizing wastewater treatment plants (WWTP) effluent were conducted to investigate fouling and filtration behaviour, especially the influence of colloidal stability on nanofiltration performance. Acidification and coagulant dosage were used to create unstable colloidal conditions. Colloidal stability of the effluent was analysed on by zeta potential measurements. A statistical design method, full factorial design with blocking, has been used to account for effluent composition variations and to account for interaction between experimental parameters. The results show a high correlation between unstable colloidal natural organic matter and the formation of dense fouling layers. The reversibility of the fouling process was shown to be independent from colloidal stability. Irreversible fouling was show to be promoted by mild acidic (pH 5) effluent conditions, whereas coagulant addition showed an increase in the reversibility of the fouling.     

Evaluation of a Modified pH‐Shift Process to Reduce 2‐Methylisoborneol and Geosmin in Spiked Catfish and Produce a Consumer Acceptable Fried Catfish Nugget‐Like Product

Abstract: Muddy and/or musty off‐flavors in farmed‐raised catfish occur as a result of the absorption of geosmin (GEO) and 2‐methylisoborneol (MIB), compounds produced by algae. Previous research suggests the acid pH‐shift method may be able to reduce off‐flavors to produce a consumer acceptable product. The objective of this research was to evaluate application of the acid pH‐shift method using a shaker sieve for protein recovery and to evaluate consumer acceptability of a resultant batter‐coated fried nugget‐like catfish product. Farm‐raised catfish were either allowed to depurate (control) or treated with 1 ppb GEO or MIB. Fillets from each replicate were collected and ground and treated by the acid pH‐shift process. Samples from all treatments and replicates were evaluated for residual GEO and MIB. In addition, batter‐coated fried catfish samples were prepared for a consumer sensory evaluation. Results demonstrated that the pH‐shift process decreased moisture, ash, and collagen content of catfish fillet tissue (P < 0.05). Flavor of control samples was preferred (P < 0.05). Texture of catfish samples treated by the pH‐shift process was preferred (P < 0.05). Results demonstrate the pH‐shift process can be utilized to reduce off‐flavors and increase the acceptability of a processed catfish product. Practical Application: Use of a sieve as an economic alternative for the pH‐shift process was evaluated for removing off‐flavors from catfish. Difficulties were encountered with regard to protein recovery using the sieve and suggestions are made to, perhaps, make the process more applicable for a sieve‐based recovery step. The process as described reduced off‐flavors, but only 2‐fold suggesting the process would work best on catfish near or just over off‐flavor thresholds. Results also indicated the pH‐shift process could be used to improve texture of a fried catfish product designed to be similar to chicken nuggets.     

Relationship between physical properties of casein micelles and rheology of skim milk concentrate

The properties of casein micelles in milk concentrates are of interest for the use of ultrafiltered (UF) skim milk concentrates in dairy products, and for the general understanding of colloidal stability and behavior of the casein micelle. The rheological behavior of UF skim milk concentrate with a casein concentration of 19.5% (wt/wt) was investigated at different pH and NaCl concentrations by analyzing flow viscometry and small amplitude oscillatory shear measurements. Viscometric flow curves were fitted to the Carreau-Yasuda model with the aim of determining values for the viscosity at infinite high shear rates and thereby estimate the voluminosity of the casein micelles (ν_casein) in the UF concentrate. The voluminosity of the casein micelles increased with addition of NaCl and decreased when pH was decreased from 6.5 to 5.5. At pH 5.2, ν_casein increased because of acid-induced aggregation of the casein micelles. The changes in ν_casein could be interpreted from transmission electron microscopy of freeze-fractured samples of the UF concentrate and partly from dynamic light scattering measurements. Altered interactions between casein micelles due to different pH and NaCl concentrations are proposed to occur due to collapse of the κ-casein layer, changed ionic strength, and altered distance between casein micelles.     

Formation of iodoacetic acids during cooking: Interaction of iodized table salt with chlorinated drinking water

Iodoacetic and chloroiodoacetic acids were formed when municipal chlorinated tap water was allowed to react with iodized (with potassium iodide) table salt or with potassium iodide itself. Iodoacetic acid was recently shown to be a potent cytotoxic and genotoxic agent. For analysis, samples were extracted with t-amyl methyl ether and converted to the corresponding methyl esters using methanol and sulfuric acid. The concentration of iodoacetic acid was determined by gas chromatography-mass spectrometry (GC-MS) using an authentic standard. The identities of iodoacetic and chloroiodoacetic acids were further confirmed by gas chromatography-high-resolution mass spectrometry (GC-HRMS). Certain influences of sodium hypochlorite and humic acid as well as the concentration of potassium iodide on the yields of these acids were investigated. The concentration of iodoacetic acid in tap water samples boiled with 2 g l^-1 of iodized table salt was found to be in the 1.5 µg l^-1 range, whilst the concentration of chloroiodoacetic acid was estimated to be three to five times lower.     

Modulation of multiple potassium currents by metabotropic glutamate receptors in neurons of the hypothalamic supraoptic nucleus

We studied the effects of activation of the metabotropic glutamate receptors on intrinsic currents of magnocellular n urons of the supraoptic nucleus (SON) with whole cell patch-clamp and conventional intracellular recordings in coronal slices (400 micron) of the rat hypothalamus. Trans-(+/-)-1-amino-1,3-cyclopentane dicarboxylic acid (trans-ACPD, 10-100 microM), a broad-spectrum metabotropic glutamate receptor agonist, evoked an inward current (18.7 +/- 3.45 pA) or a slow depolarization (7.35 +/- 4.73 mV) and a 10-30% decrease in whole cell conductance in approximately 50% of the magnocellular neurons recorded at resting membrane potential. The decrease in conductance and the inward current were caused largely by the attenuation of a resting potassium conductance because they were reduced by the replacement of intracellular potassium with an equimolar concentration of cesium or by the addition of potassium channel blockers to the extracellular medium. In some cells, trans-ACPD still elicited a small inward current after blockade of potassium currents, which was abolished by the calcium channel blocker, CdCl2. Trans-ACPD also reduced voltage-gated and Ca2+-activated K+ currents in these cells. Trans-ACPD reduced the transient outward current (IA) by 20-70% and/or the IA-mediated delay to spike generation in approximately 60% of magnocellular neurons tested. The cells that showed a reduction of IA generally also showed a 20-60% reduction in a voltage-gated, sustained outward current. Finally, trans-ACPD attenuated the Ca2+-dependent outward current responsible for the afterhyperpolarization (IAHP) in approximately 60% of cells tested. This often revealed an underlying inward current thought to be responsible for the depolarizing afterpotential seen in some magnocellular neurons. (RS)-3,5-dihydroxyphenylglycine, a group I receptor-selective agonist, mimicked the effects of trans-ACPD on the resting and voltage-gated K+ currents. (RS)-alpha-methyl-4-carboxyphenylglycine, a group I/II metabotropic glutamate receptor antagonist, blocked these effects. A group II receptor agonist, 2S,1'S,2'S-2carboxycyclopropylglycine and a group III receptor agonist, (+)-2-amino-4-phosphonobutyric acid, had no effect on the resting or voltage-gated K+ currents, indicating that the reduction of K+ currents was mediated by group I receptors. About 80% of the SON cells that were labeled immunohistochemically for vasopressin responded to metabotropic glutamate receptor activation, whereas only 33% of labeled oxytocin cells responded, suggesting that metabotropic receptors are expressed preferentially in vasopressinergic neurons. These data indicate that activation of the group I metabotropic glutamate receptors leads to an increase in the postsynaptic excitability of magnocellular neurons by blocking resting K+ currents as well as by reducing voltage-gated and Ca2+-activated K+ currents.     

The Horn Antenna as Gaussian Source in the mm-Wave Domain

Rising progress of mm-wave technology in spectroscopy, remote-sensing and its prospective application in communications requires accurate radiation beam characterization in quasi-optical systems. Gaussian approximation is a well-known practical model at optical and infrared wavelengths, and here we try to show the performance and limits of this method for the horn antenna as a classical mm-wave radiation source. This approach provides a compact and more practical analytical tool to design and optimize quasi-optical systems, compared to electromagnetic (EM) full-wave solutions. The radiation pattern (beam shape) and the phase distribution are studied analytically, both with the EM full-wave method and Gaussian approximation. Comparative results with measurements are presented to verify the analytical process and show the feasibility of the method for conventional horns.     

Cobalamin analogues modulate the growth of leukemia cells in vitro

Analogues of cyanocobalamin (CN-Cbl), with functional groups attached to either the various propionamide groups of the corrin ring or to the ribose-nucleotide linker arm, have been evaluated in a cobalamin (Cbl)-dependent in vitro cell growth assay. In this bioassay, CN-Cbl supported, in a dose-dependent manner, the growth of the murine lymphoma BW5147 and the Cbl carrier protein, human apo-transcobalamin II, reduced the required concentration of Cbl by 100-1000-fold. Any chemical modification of Cbl decreased its ability to support cellular viability and proliferation, with several of the modifications abrogating activity completely. All of the Cbl analogues that promoted growth required the presence of apo-transcobalamin II for the optimal support of cell growth. Generally, Cbl analogues modified at the d-position of the corrin ring and, to a lesser degree, analogues modified at the b- position supported cell growth, whereas analogues with modifications at the e-position did not support cell growth. Mixing experiments demonstrated an inverse order of potency of Cbl analogues to inhibit cell growth. Thus, Cbl analogues with modifications at the e-position were potent inhibitors, whereas b-analogues exhibited only partial inhibitory activity at high molar excess, and d-analogues had no inhibitory activity at all. These results indicate that modifications at the e-position of Cbl abolish the ability of Cbl to support cell growth and generate potent inhibitors of Cbl-dependent cell growth.     

Strength of soil reinforced with fiber materials (Papyrus)

Construction of building and other civil engineering structures on weak or soft soil is highly risky because such soil is susceptible to differential settlements, poor shear strength, and high compressibility. Various soil improvement techniques have been used to enhance the engineering properties of soil. Soil reinforcement by fiber material is considered an effective ground improvement method because of its cost effectiveness, easy adaptability, and reproducibility. Hence, in the present investigation, papyrus fiber has been chosen as the reinforcement material, and it was randomly included into the soil at four different percentages of fiber content, i.e., 5, 10, 15, 25% by volume of raw soil. The main objective of this research is to focus on the strength behavior of soil reinforced with randomly included papyrus fiber. Direct shear, consolidation, and displacement tests were performed on papyrusreinforced specimens with various fiber contents. The results of these tests have clearly shown a significant improvement in the failure deviator stress and shear strength parameters (c and φ) of the studied soil with a percent addition of 10% (the preferred percent). Moreover, this addition ratio reduced the displacement of the soil under loading. It can be concluded that papyrus fiber can be considered an appropriate soil reinforcement material.