Novel fluorescence sensor based on covalent immobilization of 3-amino-9-ethylcarbazole via electrostatically assembled gold nanoparticle layer

A novel technique of immobilizing indicator dyes by electrostatic adsorption and covalent bonding to fabricate optical sensors was developed. 3-Amino-9-ethylcarbazole (AEC) was attached to the outmost surface of quartz glass slide via aminosilanizing the slide, crosslinking chitosan, adsorbing Au nanoparticle, self-assembling HS(CH₂)₁₁OH, and coupling AEC. Thus, an AEC-immobilized optical sensor was obtained. The sensor exhibits a wide linear response range from 7.0×10⁻⁷ to 1.0×10⁻⁴ mol/L and a correlation coefficient of 0.995 9 for the detection of 2-nitrophenol. The detection limit and response time of the sensor are 1.0×10⁻⁷ mol/L and less than 10 s, respectively. The fluorescence intensity of the used sensor can be restored to the blank value by simply rinsing with blank buffer. A very effective matrix for immobilizing indicator dye is provided by the proposed technique, which is adaptable to other indicator dyes with amino groups besides AEC. Foundation item: Project(20775010) supported by the National Natural Science Foundation of China; Project(208095) supported by the Key Project of Ministry of Education, China; Project(07A006) supported by the Scientific Research Fund of Hunan Provincial Education Department, China; Project(07JJ3020) supported by Hunan Provincial Natural Science Foundation of China     

黄骅坳陷白水头断裂系统生长模式及其成因分析

在系统分析白水头断裂系统的断层组合、构造样式、主干断层及次级断层生长模式的基础之上,结合区域构造背景、伸展边界条件及断层间的相互作用,探讨了该断裂系统的形成机制.分析结果表明,区域构造背景下存在的伸展边界控制了整个白水头断裂系统总的构造格局.进一步证实了区内右旋走滑作用与伸展作用的相互耦合,致使次级断层沿走滑构造带出现转向或中断.断裂系统及系统内部断层间的运动学关系表明白水头断裂系统在古近纪是受两个构造动力控制,其一是地幔热作用引起岩石圈北西-南东向引张作用,其二是北北东向深断裂带的右旋剪切作用.     

政府推动下的“多中心”社区公共服务供给模式研究——基于F市N区的实证调查

随着社区建设的迅速发展,社区公共物品高效、优质供给的重要性日益凸显。如何为社区居民提供优质高效的公共服务是社区治理过程中的关键问题。在社区公共服务供给模式中引入市场、非营利组织等来解决社区公共服务供给存在不足或低效率问题。借鉴西方公共物品供给的"多中心"理论,提出政府主导型的多中心社区公共服务供给模式。     

隧道埋深及开挖方法对于二衬支护时机的影响

为了研究隧道埋深和施工方法对二衬支护时机的影响,以广梧高速公路牛车顶隧道为工程背景,建立数值计算模型,分别改变隧道埋深和施工方法,得到相应的隧道二衬支护时机.结果表明：通过数值计算,可定量得到二衬及掌子面之间的允许距离和埋深的关系;当埋深较小时,二衬与掌子面的允许距离随着埋深的增加而增加;当埋深超过某一限值时,隧道二衬与掌子面的允许距离基本不变;开挖工法对二衬与掌子面间的允许距离影响比较显著,而对仰拱与掌子面的允许距离影响很小.随着开挖断面分部的增加,开挖对围岩的扰动减小,因而可以适当延缓构筑二衬时机.若选取Ⅲ级围岩中的全断面开挖法作为参考基准,则围岩中二衬与掌子面的允许距离将由于选择不同工法需进行修正,相应的修正值为：全断面法1.0,上下台阶法和中隔壁法均为1.5.     

广义分布式天线系统上行分集性能分析

针对广泛存在于无线通信环境中的阴影效应对系统性能影响非常大的问题,采用与传统的微分集技术相比,能够有效地对抗这种阴影衰落的分布式天线技术来解决这个问题.为了获得该条件下系统的理论性能,分析了采用最大比合并和相干等增益合并分集的广义分布式天线系统.借助lognormal近似法,给出了其中断概率和误码率性能的高精度解析结果.分析表明,在存在阴影衰落的情况下,这种广义分布式天线系统能够同时获得较大的微/宏分集增益,非常适合未来的无线小区环境.     

Methane absorption and application of mixed organic aggregate prepared from Span80 and alkaline salt

The water-based materials for mine gas absorption and explosion suppression were prepared, in which the mixed organic aggregate of Span80 and alkaline salt can be used as methane absorbent. Methane was used as a model of mine gas, and the absorptions of methane with different complex materials were studied using head space gas chromatography. Then the state of aqueous material was characterized with laser light scattering instrument and the effects of different complex materials on explosion suppression were preliminarily studied in explosion chamber which can simulate mine gas explosion. The research results showed that complex material could absorb methane and there was some correlation between the mean diameter of organic aggregate in aqueous material and the absorption effect. Additionally, the aqueous material could suppress the methane explosion to some degree. The material can absorb mine gas in atomization condition, therefore, degrease mine gas concentration and influence the distribution of mine gas in the space, and then suppress the mine gas explosion to some extent.     

一种基于SRT-8算法的SIMD浮点除法器的设计与实现

在科学计算、数字信号处理、通信和图像处理等应用中,除法运算是常用的基本操作之一。基于SRT 8除法算法,设计一个SIMD结构的IEEE 754标准浮点除法器,在同一硬件平台上能够实现双精度浮点除法和两个并行的单精度浮点除法。通过优化SRT 8迭代除法结构,提出商选择和余数加法的并行处理,并采用商数字存储技术降低迭代除法的计算延时,提高频率。同时,采用复用策略减少硬件资源开销,节省面积。实验表明,在40nm工艺下,本设计综合cell面积为18601.9681 μm2,运行频率可达2.5GHz,相对传统的SRT 8实现关键延迟减少了23.81%。     

创新型嵌入式实验教学模式研究

结合军校计算机专业人才培养的具体需求,深入阐述了嵌入式计算机实验教学模式面临的问题,分析了当前军队院校实验教学现状及存在的不足,提出了嵌入式计算机实验教学的改革措施和原则,最后给出了发展创新型实验教学模式的思路。     

Excel VBA在对口中职招生考试中的应用

通过对Excel进行适当的设置,函数合理应用以及结合VBA编程,针对对口中职招生考试的实际情况,设计完成了一个界面友好、使用方便、高效的绿色电子答题平台.重点对Excel数据安全作了阐述和设计,在实际应用中取得了较好的效果.     

杀虫涂料的研究进展

从杀虫涂料的作用原理、杀虫涂料的配方设计、杀虫涂料性能评价方法三个方面对杀虫涂料的研究开发进行了论述,并对杀虫涂料研究的新进展及方向做了简要阐述。