Social organization,constraints and opportunities for kitchen garden implementation: ScalA and ScalA-FS assessment tools in Morogoro and Dodoma,Tanzania

Tanzania is the second largest country in East Africa with about 50 million inhabitants in 2014, and it is also considered as one of the poorest countries in the world. The country strongly depends on agriculture production. Like many other poor countries in sub-Saharan Africa, Tanzania sees food security as a central part of development and poverty reduction efforts. This work aims to investigate the opportunities and constraints of implementing a policy of “Kitchen Gardens” as a practice for two regions of Tanzania. The research was carried out with a qualitative approach through a first round of semi-structured interviews using a Scaling up Assessment Tool (ScalA), and a second round with a questionnaire survey, using a Scaling up Assessment Tool for Food Security (ScalA-FS) by Tanzanian and German experts from the Trans-SEC project. The experts assessed implementation suitability and the institutional requirements of Kitchen Garden across the food value chains in two Tanzanian regions with different climate regions, namely Dodoma (semi-arid) and Morogoro (sub-humid). Kitchen Garden assessments did not differ significantly between these regions. The ScalA tools provided a range of statements that allowed an overview of the structural situation to be obtained, which could enable Kitchen Garden activity to be incentivized and scaled up. However, a number of specific aspects, potentials, challenges, and likely bottlenecks of implementation related to their feasibility and institutional requirements, were indicated, which should be carefully monitored during implementation. Adopting the recommended strategies could help to close gaps in implementation, enhance community empowerment and social network development, reduce food insecurity and improve the health of the communities.     

适度硬化表面淬火钢的加工和应用(一)

表面淬火钢的硬化程度对变速器零件的加工和应用起到决定性的作用.硬化程度主要取决于材料的化学成分,是选择表面淬火钢淬火温度的主要标准,因为稳定的材料质量是高效率大规模生产的保证.顶端淬火样品的变形和热处理条件对测量结果具有决定性的影响.由于明显的不确定性,必须考虑到顶端淬火试验测定的硬化程度和材料化学成分之间的不同.在狭窄的硬化程度范围内对表面淬火钢的热处理确保了可预测和可再现的大规模生产.一些实例表明,由于稍稍偏离所需的硬化程度范围造成不规则的热处理变形,并因成本问题而被放弃.     

Vitamin D Reverses Disruption of Gut Epithelial Barrier Function Caused by Campylobacter jejuni

Infections by the zoonotic foodborne bacterium Campylobacter jejuni (C. jejuni) are among the most frequent causes of bacterial gastroenteritis worldwide. The aim was to evaluate the relationship between epithelial barrier disruption, mucosal immune activation, and vitamin D (VD) treatment during C. jejuni infection, using intestinal epithelial cells and mouse models focused on the interaction of C. jejuni with the VD signaling pathway and VD treatment to improve C. jejuni-induced barrier dysfunction. Our RNA-Seq data from campylobacteriosis patients demonstrate inhibition of VD receptor (VDR) downstream targets, consistent with suppression of immune function. Barrier-preserving effects of VD addition were identified in C. jejuni-infected epithelial cells and IL-10^−/− mice. Furthermore, interference of C. jejuni with the VDR pathway was shown via VDR/retinoid X receptor (RXR) interaction. Paracellular leakiness of infected epithelia correlated with tight junction (TJ) protein redistribution off the TJ domain and apoptosis induction. Supplementation with VD reversed barrier impairment and prevented inhibition of the VDR pathway, as shown by restoration of transepithelial electrical resistance and fluorescein (332 Da) permeability. We conclude that VD treatment restores gut epithelial barrier functionality and decreases bacterial transmigration and might, therefore, be a promising compound for C. jejuni treatment in humans and animals.     

Highly Active and Diastereoselective N,O‐ and N,N‐Yttrium Complexes for Intramolecular Hydroamination

The intramolecular hydroamination of aminoalkynes and unactivated aminoalkenes catalyzed by yttrium N,O‐ and N,N‐complexes has been investigated. The N,N‐yttrium complexes are highly active, catalyzing the conversion of a wide range of terminal aminoalkenes at room temperature, and internal aminoalkenes at elevated temperature, to yield pyrrolidine and piperidine products in high yields. A high diastereoselectivity of up to 23:1 is observed at 0 °C with 1‐methyl‐4‐pentenylamine as substrate.     

Efficient Trifluoromethylation of Activated and Non‐Activated Alkenyl Halides by Using (Trifluoromethyl)trimethylsilane

An efficient method for the trifluoromethylation of halogenated double bonds by using in situ generated “trifluoromethyl copper” is described. Herein, the most common trifluoromethyl source, (trifluoromethyl)trimethylsilane, was converted selectively into “trifluoromethyl copper” by using copper iodide as copper source and potassium fluoride as promoter. In 1,3‐dimethyl‐3,4,5,6‐tetrahydro‐2(1H)‐pyrimidinone (DMPU) as chelating solvent the trifluoromethylation of activated and non‐activated alkenyl halides occurred mostly in good to excellent yields in up to a gram scale.     

Remarkable findings concerning PBDEs in the terrestrial top-predator red fox (Vulpes vulpes)

In the present study, we have analyzed muscle, liver, and adipose tissue of 33 red foxes from Belgium for their content of polybrominated diphenyl ethers (PBDEs). Median sums of seven tri- to hepta-BDEs (BDE 28, BDE 47, BDE 99, BDE 100, BDE 153, BDE 154, and BDE 183) were 2.2, 2.4, and 3.4 ng/g lipid weight in adipose tissue, liver, and muscle, respectively. These levels were lower than those found in various species of voles and mice, the main prey species of the red fox. This is probably related to the high capacity of the foxes to metabolize and eliminate lower brominated congeners. BDE 209 generally dominated the PBDE congener profiles in the red fox samples. In samples containing BDE 209, this congener contributed, on the average, approximately 70% to the total PBDE content. BDE 209 was measured in concentrations as high as 760 ng/g lipid weight in the liver, but the detection frequency was not more than 40%. In animals with the highest BDE 209 levels, this congener was detected in muscle, liver, as well as in adipose tissue. Other abundant congeners were BDE 153 and BDE 47, which prevail in other terrestrial species. The particular PBDE congener profile observed in the red fox resembles that seen in grizzly bears from Canada, but differs from those previously reported for terrestrial avian species. Our data confirms unambiguously that BDE 209 does bioaccumulate in terrestrial top predators, such as the red fox.     

On-line reaction monitoring in the liquid phase using two mid-infrared quantum cascade lasers simultaneously

On-line monitoring of a model reaction was performed by employing two pulsed mid-infrared Fabry-Pérot quantum cascade lasers (QCL). The emission maxima of the QCLs were located at 1393 and 1080 cm(-1). An optical system of parabolic mirrors and a ZnSe beam splitter combined the two laser beams and allowed a transmission cell to be probed with both QCLs simultaneously. The reaction mixture was pumped continuously through a cell that had an optical path of 48 microm. This dual QCL system allowed fast absorption measurements of the reaction mixture at two distinct wavenumbers. The reaction under study was the oxidation of sulfite to sulfate with hydrogen peroxide acting as oxidant. On-line measurements of the chemical reaction allowed direct, real-time monitoring of sulfate formation and hydrogen peroxide depletion.     

Parallel manipulation of bifunctional DNA molecules on structured surfaces using kinesin-driven microtubules

We have developed a technique to manipulate bifunctional DNA molecules: One end is thiolated to bind to a patterned gold surface and the other end is biotinylated to bind to a microtubule gliding over a kinesin-coated surface. We found that DNA molecules can be stretched and overstretched between the gold pads and the motile microtubules, and that they can form dynamic networks. This serves as a proof-of-principle that biological machineries can be used in vitro to accomplish the parallel formation of structured DNA templates that will have applications in biophysics and nanoelectronics.     

Natural products from marine organisms and their associated microbes

The marine environment is distinguished by unique groups of organisms being the source of a wide array of fascinating structures. The enormous biodiversity of marine habitats is mirrored by the molecular diversity of secondary metabolites found in marine animals, plants and microbes. The recognition that many marine invertebrates contain endo- and epibiotic microorganisms and that some invertebrate-derived natural products are structurally related to bacterial metabolites suggests a microbial origin for some of these compounds. Other marine natural products, however, are clearly located in invertebrate tissue and microbial involvement in the biosynthetic process seems unlikely. The complexity of associations in marine organisms, especially in sponges, bryozoans and tunicates, makes it extremely difficult to definitively state the biosynthetic source of many marine natural products or to deduce their ecological significance. Whereas many symbiotic marine microorganisms cannot be isolated and cultured, numerous epi- and endobiotic marine fungi produce novel secondary metabolites in laboratory cultures. The potent biological activity of many marine natural products is of relevance for their ecological function but is also the basis of their biomedical importance.