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11.
Structure and function of animal fatty acid synthase 总被引:1,自引:0,他引:1
Fatty acid synthase (FAS; EC 2.3.1.85) of animal tissues is a complex multifunctional enzyme consisting of two identical monomers. The FAS monomer (approximately 270 kDa) contains six catalytic activities and from the N-terminus the order is beta-ketoacyl synthase (KS), acetyl/malonyl transacylase (AT/MT), beta-hydroxyacyl dehydratase (DH), enoyl reductase (ER), beta-ketoacyl reductase (KR), acyl carrier protein (ACP), and thioesterase (TE). Although the FAS monomer contains all the activities needed for palmitate synthesis, only the dimer form of the synthase is functional. Both the biochemical analyses and the small-angle neutron-scattering analysis determined that in the dimer form of the enzyme the monomers are arranged in a head-to-tail manner generating two centers for palmitate synthesis. Further, these analyses also suggested that the component activities of the monomer are organized in three domains. Domain I contains KS, AT/MT, and DH, domain II contains ER, KR, and ACP, and domain III contains TE. Approximately one fourth of the monomer protein located between domains I and II contains no catalytic activities and is called the interdomain/core region. This region plays an important role in the dimer formation. Electron cryomicrographic analyses of FAS revealed a quaternary structure at approximately 19 A resolution, containing two monomers (180 x 130 x 75 A) that are separated by about 19 A, and arranged in an antiparallel fashion, which is consistent with biochemical and neutron-scattering data. The monomers are connected at the middle by a hinge generating two clefts that may be the two active centers of fatty acid synthesis. Normal mode analysis predicted that the intersubunit hinge region and the intrasubunit hinge located between domains II and III are highly flexible. Analysis of FAS particle images by using a simultaneous multiple model single particle refinement method confirmed that FAS structure exists in various conformational states. Attempts to get higher resolution of the structure are under way. 相似文献
12.
The indium doped silver oxide thin films have been prepared at 275 °C on soda lime glass and silicon substrates by reactive
electron beam evaporation technique; the deposition rate has been varied (by varying the electron beam current) in the range
0.94–16.88 nm/s keeping the oxygen flow rate constant. These films are polycrystalline. The electrical resistivity for these
films decreases with increasing deposition rate. The AIO films prepared with a deposition rate of 5.7 nm/s show near p-type
conductivity. The work function has been measured on these films by contact potential method using Kelvin Probe. The surface
morphology of the films has been evaluated using atomic force microscopy (AFM). The roles of indium doping and oxygen vacancies
in the electrical properties of these films have been analyzed; the ionized impurity scattering is the dominant mechanism
controlling the electrical conduction in these films. 相似文献
13.
V. S. N. Murty B. Subrahmanyam L. V. Gangadhara Rao G. V. REDDY 《International journal of remote sensing》2013,34(12):2361-2372
Monthly maps of sea surface temperature (SST) derived from NOAA (National Oceanic and Atmospheric Administration)-AVHRR (Advanced Very High Resolution Radiometer) data during 1992 for the Bay of Bengal are analysed and compared with the available/compiled monthly seatruth (bucket thermometer) data of this region. It was noticed that the computed SST bias (AVHRR SST minus Seatruth SST), in general, varied between 2.0 and 2.5 C with smaller bias values (1.5 to 1.5 C) during January-June and December. Larger bias values were noticed in the south-eastern Bay in July and in the Andaman Sea in October. The large SST biases suggested the necessity for improvement of SST algorithms by properly removing the clouds. The spatial variation of Standard Deviation of SST bias was particularly high (0.7) in the western Bay when compared to other parts of the Bay of Bengal. The monthly maps of AVHRR SST clearly depicted the seasonal cycle of SST showing the well known bi-modal SST distribution of the study region with winter cooling, summer heating, monsoonal cooling and post-monsoon warming phases. The seasonal cycle of SST further revealed the persistence of Warm Pool (SST 28 C) in the Bay of Bengal from March through October. 相似文献
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M. Subrahmanyam P. Boule V. Durga Kumari D. Naveen Kumar M. Sancelme A. Rachel 《Solar Energy》2008,82(12):1099-1106
Bactericidal and organic degradation effects of TiO2 on pumice stone are described in this paper. Immobilization of TiO2 on pumice stone is easy and efficient method to obtain photocatalytic reactions without the problem of filtration. Pumice stone is soft and available as pellets that can be used in pellets fixed (with cement or poly carbonate) on a slanting plank/glass by coating the preformed TiO2 over the pellets using simple paint brush for applying the photo catalyst. The treatment of inactivation of bacteria especially E. coli existing in real river waters and also different model organic substrate degradations like acid orange-7, resorcinol, 4, 6-dinitro-o-cresol, 4-nitrotoluene-2-sulfonicacid, isoproturan are studied. Furthermore, TiO2 over pumice stone loaded in a multi tube reactor gave similar results for the disinfection and detoxification studies. 相似文献
17.
Umaid Singh Paleti Venkateswara Rao Nukala Subrahmanyam Kulbhushan Saxena 《Journal of the science of food and agriculture》1993,61(4):395-400
Eight newly developed pigeonpea genotypes (ICPL 87, ICPL 151, ICPL 270, ICPL 366, ICPL 87051, ICPL 87063, ICPL 87067, and ICPV 1), and the two controls (BDN 2 and C 11) were analysed for cooking quality parameters and chemical composition, including amino acids and minerals. Protein quality was evaluated by determining the true protein digestibility, biological value, net protein utilisation (NPU), and utilisable protein. These genotypes differed significantly (P < 0–01) in the dhal cooking time. Sensory properties of dhal of these genotypes were found to be within the acceptable range, even though there were considerable differences among genotypes. Dhal protein, calcium, magnesium, zinc, and iron contents of these genotypes showed noticeable differences. Calcium content of ICPL 87067 was the highest (85-6 mg per 100 g) and of ICPL 87 the lowest (54-4 mg per 100 g) indicating large differences among the newly developed genotypes. No noticeable differences in sulphur-containing amino acids of these genotypes were observed. NPU was the highest (65–4%) for ICPL 366 and the lowest (56–6%) for ICPL 270 and ICPL 87067 indicating significant (P < 0–01) differences among genotypes studied. 相似文献
18.
Highly reliable thin oxynitride layers of very good Si-SiO2 interface endurance were grown on silicon wafers with a split N 2O cycle (N2O/O2/N2O) employing rapid thermal processing (RTP). Excellent electrical characteristics with reduced positive charge generation, electron trapping and/or interface state generation were achieved under high field stressing compared to pure N2O dielectric 相似文献
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A. R. James J. Subrahmanyam 《Journal of Materials Science: Materials in Electronics》2006,17(7):529-535
Lead lanthanum zirconate titanate with the nominal composition (Pb0.93La0.07)(Zr0.60Ti0.40)O3 [designated as PLZT (7/60/40)] was synthesised from the constituent oxides by a mechanochemical processing route (MCP). Besides
obtaining nano-sized powders, partial phase formation of the desired PLZT composition could be achieved at room temperature.
DTA-TGA analyses were used to study the effect of reducing the particle size on the reaction kinetics of PLZT and thermomechanical
studies were used to understand the sintering mechanism of the nano-scale powder compacts. Fully dense ceramics could be achieved
at significantly reduced sintering temperatures compared to their conventionally synthesized counterparts. Transmission and
scanning electron microscopy were employed to study the microstructure of the powders and CIP-sintered compacts, respectively.
Resonance data were acquired in order to compute the electromechanical properties. Besides reduction in sintering temperatures,
there are differences in electrical properties obtained on PLZT ceramics synthesized by MCP and conventionally processed samples. 相似文献