Ink-Jet Printing System?An Analytical Computer Model

An analytical computer model of an ink jet printing system was created. Based upon the values of 13 input parameters, the program delivers predictions of the forces acting on individual drops and their resulting dynamic behavior. Predictions of drop displacements and flight times were used to infer drop pattern appearance resulting from a given input signal. The computer model was based upon theoretical work plus extensive empirical measurements. Drop behavior was predicted with modest accuracy. An improved understanding of ink jet printing systems and numerous recommendations for their use were also derived in this study.     

Studies on the ultrastructure, histochemistry and cytochemistry of the uninfected digestive gland of Bithynia tentaculata (Mollusca: Gastropoda) and on the ultrastructure of this host organ in snails infected with larval digeneans

The structure and function of the digestive gland of the gastropod mollusc, Bithynia tentaculata, was investigated using ultrastructural, histochemical, and cytochemical techniques. The digestive gland was shown to be composed of two main cell types, the "digestive" cells and "secretory" cells. The digestive cells appeared to be concerned with the absorption and digestion of nutrients, while secretory cells produced digestive enzymes and calcareous concretions. Undifferentiated cells were scattered between these two main cell types. The pathological effects of larval digeneans on the digestive gland were also investigated, at the ultrastructural level. In such infected snails the digestive gland appeared to be degenerating. The significance of this tissue destruction was briefly discussed.     

Learning from designing the RoboFesta—Blue Peter robots

Can children and adults learn anything from building robots? In 2001 we ran a robot design competition with the BBC TelevisionBlue Peter, program. Some 32 000 children, aged between 5 and 16 years, submitted paper designs. We built prototypes of the four winners' robots for a Robot Design MasterClass at the London Science Museum. It is widely believed that across the curriculum, robotics is a powerful force in motivating education. We ask what the children learned from the robot design process. Our answer is that they learned many things, both inside and outside the science and technology curriculum.     

Phase I IASC-ASCE Structural Health Monitoring Benchmark Problem Using Simulated Data

Structural health monitoring (SHM) is a promising field with widespread application in civil engineering. Structural health monitoring has the potential to make structures safer by observing both long-term structural changes and immediate postdisaster damage. However, the many SHM studies in the literature apply different monitoring methods to different structures, making side-by-side comparison of the methods difficult. This paper details the first phase in a benchmark SHM problem organized under the auspices of the IASC–ASCE Structural Health Monitoring Task Group. The scale-model structure adopted for use in this benchmark problem is described. Then, two analytical models based on the structure—one a 12 degree of freedom (DOF) shear-building model, the other a 120-DOF model, both finite element based—are given. The damage patterns to be identified are listed as well as the types and number of sensors, magnitude of sensor noise, and so forth. MATLAB computer codes to generate the response data for the various cases are explained. The codes, as well as details of the ongoing Task Group activities, are available on the Task Group web site at 〈http://wusceel.cive.wustl.edu/asce.shm/〉.     

Labelled-replica techniques: post-shadow labelling of intramembrane particles in freeze-fracture replicas

Three methods are described for direct post-fracture, post-shadow labelling of individual classes of intramembrane particles (IMPs) in freeze-fracture replicas of biological membranes. The P-face IMPs corresponding to the acetylcholine receptor complexes (AChRs) of vertebrate neuroeffector junctions are identified by post-replication labelling with ferritin-antibody complexes and with neurotoxin-biotin-avidin-colloidal gold affinity ligands. (The freeze-etch nomenclature of Branton et al., 1975, is used in this report.) These post-shadow labelling techniques resemble conventional en bloc labelling techniques except that the labelling reagents must penetrate a thin but discontinuous layer of platinum superimposed on the molecules of interest. In the ‘sectioned labelled-replica technique’, the replicated and labelled tissues are stained, embedded in plastic and sectioned parallel to the replica-tissue interfaces. In the direct ‘labelled-replica techniques’, the replicated and labelled samples are freeze-dried or critical point dried, the labelled surfaces are stabilized by carbon coating, and the underlying tissues are dissolved, allowing the labelled-replicas to be examined as conventional freeze-fracture replicas. The unshadowed side of each AChR IMP is shown to retain sufficient biochemical information to permit both immunospecific and neurotoxin specific labelling despite formaldehyde fixation, freezing, fracturing, platinum shadowing, and thawing in aqueous media. A new mixed ferricyanide-osmium staining method reveals electron opaque structures spanning the membrane bilayer in the same size, number and distribution as the labelled IMPs. These experiments demonstrate the feasibility of identifying individual IMPs in freeze-fracture replicas and may allow the identification of specific membrane lesions in human disease.     

Detection by a solid phase independent enzyme immunoassay of monoclonal anti-idiotypic antibodies against monoclonal antibodies neutralizing Semliki Forest virus and encephalomyocarditis virus

3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (EC 1.1.1.34) is the rate limiting step in the mevalonate pathway that produces isoprenoids and cholesterol. Inhibitors of HMG-CoA reductase are teratogenic in vivo and induce neural tube defects in rat embryo culture, effects which appear unrelated to cholesterol deficiency. This study is the first to localize HMG-CoA reductase mRNA by in situ hybridization (ISH). Expression of reductase mRNA was examined in post-implantation rat embryos, and for control purposes in rat liver and UT-1 cells, using a digoxigenin-11 (dig-11) labelled cRNA probe. Eighteen-day fetal liver showed heavy but patchy hybridization, and adult rat liver showed strong hybridization only on some periportal hepatocytes, which was absent in livers of fasted animals. UT-1 cells stimulated to overexpress HMG-CoA reductase mRNA were strongly positive with the same probe. Control hybridizations with sense strand RNA probe, or with cRNA probe on pre-RNased tissue were negative. Strong hybridization signal for HMG-CoA reductase mRNA was observed in all tissues of the post-implantation rat embryo, from egg cylinder to 30 somite stages (7 to 12 days). Heavy signal was noted in primitive ectoderm and neural tube. The wide embryonic and extraembryonic distribution and abundance of HMG-CoA reductase mRNA may reflect developmental requirements for products of the mevalonate pathway, e.g., isoprenoids for post-translational farnesylation of p21ras.     

When viewing variations in paintings by Mondrian, aesthetic preferences correlate with pupil size.

Observers consciously prefer Mondrian's paintings in their original orientation compared with a rotated position—the “oblique effect” (Latto, Brain, & Kelly, 2000). However, this finding's premise, that all vertical–horizontal orientations of the thick black lines in Mondrian's oeuvre are preferred, overlooks the fact that the overall balance of these images is also altered when they are reoriented. Thus, balance may regulate the oblique effect, which might influence conscious aesthetic preferences. To address this issue, we explore Hess's (1965, 1972) claim that observers will unconsciously increase their pupil diameter to pleasing images and constrict it to unpleasant images. We overcame Hess's methodological limitation of not keeping his images' luminances and contrast constant across conditions by presenting eight Mondrian paintings (1921–1944) to 30 observers on a CRT for 20 s each in either their original or seven rotated positions. Simultaneously, we measured their pupil size while asking them to report how (dis)pleasing they found each image. We found both evidence for the oblique effect (where image rotation hampers preference) and a correlation between this consciously reported aesthetic preference and unconsciously derived pupil size. (PsycINFO Database Record (c) 2010 APA, all rights reserved)