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排序方式: 共有2317条查询结果,搜索用时 15 毫秒
61.
TA Bateman RJ Zimmerman RA Ayers VL Ferguson SK Chapes SJ Simske 《Canadian Metallurgical Quarterly》1998,23(6):527-535
Pituitary adenylate cyclase activating peptide and vasoactive intestinal peptide belong to the same neuropeptide family. Both peptides are present in nerve fibers in the gastric wall and are thought to be involved in the regulation of inflammatory processes. Experimental ulcers were induced in the rat gastric mucosa by local application of acetic acid. During the healing process we examined the PACAP and VIP innervation by means of immunohistochemistry and in situ hybridization. The ulcer area was examined from day 1 to day 15 after ulcer induction. There was a marked depletion of PACAP in nerve fibers at the ulcer margin from day 1 and onwards. On day 10 and day 15, PACAP-immunoreactive nerve fibers could again be visualized at the ulcer margin. In contrast, VIP immunoreactive nerve fibers were present at the ulcer margin at all time points studied. From day 10 following ulcer induction PACAP- and VIP- immunoreactive nerve fibers were increased in frequency in the smooth muscle beneath the ulcer. An upregulation of VIP and PACAP mRNA was also demonstrated in the myenteric ganglia adjacent to ulcer. The present results indicate that neuronal PACAP and VIP react differently to the inflammation at the ulcer margin but similarly in the smooth muscle during the ulcer healing. 相似文献
62.
E Krementsova MJ Giffin D Pincus TA Baker 《Canadian Metallurgical Quarterly》1998,273(47):31358-31365
Mu transposase is a member of a protein family that includes many transposases and the retroviral integrases. These recombinases catalyze the DNA cleavage and joining reactions essential for transpositional recombination. Here we demonstrate that, consistent with structural predictions, aspartate 336 of Mu transposase is required for catalysis of both DNA cleavage and DNA joining. This residue, although located 55 rather than 35 residues NH2-terminal of the essential glutamate, is undoubtedly the analog of the second aspartate of the Asp-Asp-35-Glu motif found in other family members. The core domain of Mu transposase consists of two subdomains: the NH2-terminal subdomain (IIA) contains the conserved Asp-Asp-Glu motif residues, whereas the smaller COOH-terminal subdomain (IIB) contains a large positively charged region exposed on its surface. To probe the function of domain IIB, we constructed mutant proteins carrying deletion or substitution mutations within this region. The activity of the deletion proteins revealed that domains IIA and IIB can be provided by different subunits in the transposase tetramer. Substitution mutations at two pairs of exposed lysine residues within the positively charged surface of domain IIB render transposase defective in transposition at a reaction step after DNA cleavage but prior to DNA joining. The severity of this defect depends on the structure of the DNA flanking the cleavage site. Thus, these data suggest that domain IIB is involved in manipulating the DNA near the cleavage site and that this function is important during the transition between the DNA cleavage and the DNA joining steps of recombination. 相似文献
63.
塔程程 《吉林化工学院学报》2021,38(8):76-79
信息检索课程的系统化讲授,对于大学生优化信息检索行为,提高信息检索使用效率具有不容忽视的教学辅助作用。立足问卷调查形成的一系列相关数据,探讨大学生信息检索行为存在问题,最终在课程教学中提出优化大学生信息检索的对策:明确信息检索目的,提升实施效果;有效提升检索行为实施频率,增强综合质量;使得信息获取多元发展,优化成效凸显。 相似文献
64.
65.
为探求黄池沟退水道弯道的合理体型,采用正交设计与数值模拟相结合的方法研究了体型参数对弯道水流的影响。选取弯道宽度、弯道曲率半径、纵向坡度3个主要影响参数按正交试验设计,确定了9组正交试验方案,利用经水工模型试验结果验证的数学模型对正交试验方案进行模拟,分析了体型参数对弯道及下游陡坡段横向水位差的影响。结果表明,3个体型参数影响作用由大到小依次为纵向坡度、弯道宽度和弯道曲率半径;坡度为显著影响因素,且平坡最佳,弯道曲率半径和弯道宽度影响不显著,据此推荐合理体型参数为坡度取0,弯道宽度和弯道曲率半径采用原设计值。 相似文献
66.
离子束增强沉积氮化硅膜及TiAl抗高温氧化性能的改善 总被引:3,自引:0,他引:3
用离子束增强沉积(IBED)方法在金属间化合物TiAl上合成厚度为0.5,1,和2μm的氮化硅薄膜。所形成的薄膜为非晶态,膜与基底间存在混合的过渡区,膜与基底结合紧密用AES,XRD和XPS研究薄膜的组成和结构,高温循环氧化结果表明,经沉积膜的TiAl试样的抗氧化性能显著提高其中沉积0.5μm薄膜的试样表现出极好的抗循环氧化性能由SEM及EDS分析得出,良好的高温稳定性能、高的膜/基底结合力和形成富Al2O3和硅化物的保护层是提高TiAl抗高温氧化性能的主要因素. 相似文献
67.
为探明黄河内蒙古段沉积物中砷含量及形态分布特征,采集黄河内蒙古段干流柱状沉积物,采用HNO4-H2SO4-HCl O4法消解沉积物样品,提取沉积物中吸附型砷(A-As)、铝型砷(Al-As)、铁型砷(Fe-As)、钙型砷(CaAs)、包蔽型砷(O-As)5种形态砷,并采用氢化物发生原子荧光分光光度法测定砷含量。结果表明:黄河内蒙古段干流柱状沉积物中砷含量为7.123~16.661 mg/kg,平均为9.292 mg/kg;从整体上看,左岸沉积物砷含量高于右岸,随深度增加砷含量呈下降趋势,从上游至下游砷含量在黑柳子段出现高值;黄河内蒙古段沉积物中砷以Ca-As为主,O-As次之;随着深度的增加,总体上,Ca-As含量略有升高,A-As和O-As含量有所下降,Al-As和Fe-As含量基本不变。 相似文献
68.
69.
A new type of a swinging interferometer, the carousel interferometer, is presented, and its properties are studied and compared with other swinging interferometers. The new interferometer is built with five plane mirrors. The optical path difference is accomplished by rotation of a system that consists of four mirrors. The modulation is almost independent of the scanning of the interferometer. It is not sensitive to external perturbations such as bending or other deformations of the mount. The construction is very compact. Because of its stability and low cost it is very applicable to small Fourier-transform spectrometers for any wave-number region from the far infrared down to the ultraviolet. 相似文献
70.
Periovular granuloma formation during Schistosoma mansoni infection is a complex, multifaceted immunologic response. Products of arachidonic acid metabolism have been shown to contribute to this response through studies in which general inhibitors of lipoxygenase function reduce granulomatous inflammation. To determine which lipoxygenases are important for granuloma development in schistosomiasis, wild type mice or mice deficient for 5-lipoxygenase (5-LO) or "leukocyte-type" 12-lipoxygenase (12-LO) were infected with S. mansoni and studied for responses to schistosome eggs and egg antigens. At the acute stage of infection, when granuloma formation is usually maximal, 5-LO deficient mice developed smaller granulomas around liver-deposited schistosome eggs compared with wild type or 12-LO deficient mice. 5-LO mice also displayed less antibody-mediated (5 h) and cell-mediated, delayed-type (24 h) hypersensitivity to schistosome egg antigens than did the other two infection groups. In an attempt to determine possible mechanisms for the reduced inflammatory responses, we also measured hepatic mRNA levels of cytokines that have been shown to influence granuloma size (IL-4, IL-10, and IFN-gamma). The mRNA levels for IL-10 were significantly lower in 5-LO-deficient mice, but SEA-stimulated spleen cells did not demonstrate a significant difference in IL-10 production between wild type and 5-LO mice. These data suggest that 5-LO plays a role in host responses to schistosomiasis via a mechanism that cannot be explained solely by changes in expression of these cytokines. 相似文献