Amphiphilic Interpenetrating Networks for the Delivery of Hydrophobic, Low Molecular Weight Therapeutic Agents

To investigate the delivery of hydrophobic therapeutic agents, a novel class of interpenetrating networks (IPNs) were synthesized and composed of two networks: methacrylic acid grafted with poly(ethylene glycol) tethers, P(MAA-g-EG), and poly(n-butyl acrylate) (PBA). The hydrophilic P(MAA-g-EG) networks are pH-responsive hydrogels capable of triggered release of an encapsulated therapeutic agent, such as a low molecular weight drug or a protein, when it passes from the stomach (low pH) to upper small intestine (neutral pH). PBA is a hydrophobic homopolymer that can affect the IPN swelling behavior, the therapeutic agent loading efficiencies in IPNs, and solute release profiles from IPNs. In dynamic swelling conditions, IPNs had greater swelling ratios than P(MAA-g-EG), but in equilibrium swelling conditions the IPN swelling ratio decreased with increasing PBA content. Loading efficiencies of the model therapeutic agent fluorescein ranged from 21 - 44%. Release studies from neat P(MAA-g-EG) and the ensuing IPNs indicated that the transition from low pH (2.0) to neutral pH (7.0) triggered fluorescein release. Maximum fluorescein release depended on the structure and hydrophilicity of the carriers used in these studies.     

Team-oriented software practicum

New computer science graduates are inadequately prepared to apply their abstract knowledge, and they have rarely worked in the teams required in modern business. To remedy these shortcomings and alleviate the low motivation that often accompanies the first years of college, we proposed that a pilot group of entering freshmen should be formed into a four-year experimental team. Unlike traditional course-at-a-time approaches, this supplements the existing curriculum by integrating material across courses through team construction of software projects. Under the guidance of faculty and graduate student mentors, students work cooperatively on projects related not just to programming but to the entire lifecycle of software production, from market analysis to revision based on technical support. Initial projects are team-oriented and scaled to the capabilities of entering students while final projects span the product development cycle and involve several semesters of effort. Industrial representatives provide a practical perspective by presenting seminars on special topics and evaluating student projects in light of professional standards. This type of experiment provides the industrial community with students better prepared to face the challenges of professional software development. It also offers consolidated learning, enhanced student retention, significant student-faculty involvement, and the potential to identify learning experiences that may be usefully integrated into existing courses     

Illumination with solid state lighting technology

High-power light-emitting diodes (LEDs) have begun to differentiate themselves from their more common cousins the indicator LED. Today these LEDs are designed to generate 10-100 lm per LED with efficiencies that surpass incandescent and halogen bulbs. After a summary of the motivation for the development of the high-power LED and a look at the future markets, we describe the current state of high-power LED technology and the challenges that lay ahead for development of a true "solid state lamp." We demonstrate record performance and reliability for high-power colored and white LEDs and show results from the worlds first 100-plus lumen white LED lamp, the solid state equivalent of Thomas Edison's 20-W incandescent lightbulb approximately one century later     

A flexible graphical user interface for embedding heterogeneousneural network simulators

A graphical user interface (GUI) for heterogeneous neural network simulators proposed in this article is intended to be of use both for the novice and for the experienced neural network user. For the novice, it provides an easy-to-use neural network simulation package that insulates the user from the requirements of knowing the simulator implementation details or the configuration file syntax. For the experienced neural network professional it provides an interface that is easily extensible to include any additional neural network simulator in its binary form. To satisfy both academic and personal computer environments, the GUI has been developed by using the free TCL/TK software package, available on workstations running Unix and on PCs running the free Linux operating system. Although the GUI and the embedded simulators have been successfully tested both in neural network research and training programs, a more extensive testing in undergraduate and graduate level classes is in progress     

Arcjet flow properties determined from laser-induced fluorescence of atomic nitrogen

Flow property measurements that were recently acquired in the Ames Research Center Aerodynamic Heating Facility arcjet using two-photon laser-induced fluorescence (LIF) of atomic nitrogen (N) are reported. The flow properties, which include velocity, translational temperature, and N concentration, were measured simultaneously over a range of facility operating conditions for N(2)-argon test gas flows in the 30-cm-diameter nozzle. A recent measurement of the two-photon excitation cross section for the 3p(4)D degrees <-- 2p(4)S degrees transition of atomic nitrogen is used to convert the relative nitrogen concentration measurements to absolute values, and a nitrogen flow reactor is used to provide a room-temperature, reference-wavelength calibration of the translational temperature and velocity measurements. When combined with information from facility measurements, an analysis of the flow properties obtained using two-photon LIF of N yields the total free-stream flow enthalpy.     

A simplified modeling approach using microbial growth kinetics for predicting exposure concentrations of organic chemicals in treated wastewater effluents

Various mathematical relationships have been used to assess exposure concentrations of organic chemicals when emissions occur via wastewater treatment. These relationships range from a simple removal factor calculation to more sophisticated approaches using kinetic based mathematical models. While these existing approaches have been used by decision makers to screen new chemicals for exposure assessments, they all have limitations in the predictive capabilities. Thus, a simplified modeling approach grounded in sound scientific fundamentals that utilizes relatively easy to obtain input parameters is needed. In this paper a simplified modeling approach that utilizes microbial growth kinetics was developed for predicting effluent concentrations in secondary biological wastewater treatment systems. Receiving water predicted exposure concentrations (PEC) are assessed by using a dilution factor. One advantage of this approach is that it allows for wastewater treatment plant effluent concentrations, and therefore receiving water exposure levels, to be predicted with a minimum amount of experimental data. It also provides quantitative data that can be used to assess the relative biodegradability of different chemicals for use in regulatory and risk assessment activities.     

Diabetic-like retinopathy: early and late intervention therapies in galactose-fed rats

PURPOSE: To determine whether the diabetic-like thickening of retinal capillary basement membrane (RCBM) that develops in the galactose-fed rat model of diabetic ocular complications could be halted or ameliorated after 4 or 8 months of galactosemia by treatment with ARI-509, a potent new aldose reductase inhibitor (ARI), or by withdrawal of the galactose diet. METHODS: Weanling female Sprague-Dawley rats were randomized into eight groups and fed laboratory chow plus 50% starch, control group (CON); 50% D-galactose, galactose-fed group (GAL); 50% D-galactose with ARI-509 at 25 mg/kg or 10 mg/kg body wt per day, high-dose prevention group (HDP) and low-dose prevention group (LDP), respectively; 50% D-galactose for 4 or 8 months and then intervention by addition of ARI-509 (25 mg/kg body wt per day), 4-month intervention group (4IN) and 8-month intervention group (8IN), respectively; or 50% D-galactose for 4 or 8 months and then intervention by withdrawing galactose and replacing it with the 50% starch diet, 4-month galactose withdrawal group (4GW) and 8-month galactose withdrawal group (8GW), respectively. After 4, 8, 16, and 24 months of the experimental diets, the levels of carbohydrates in tissues and the extent of RCBM thickening in capillaries of the outer plexiform layer were determined in all groups. RESULTS: Retinal polyol was reduced by 95% in all ARI-treated groups and by 100% in the 4GW and 8GW groups after withdrawal of the galactose. The mean RCBM thickness increased rapidly in GAL rats, becoming almost two times greater (189 +/- 9.4 nm) than in CON rats (103 +/- 3.4 nm) by 24 months. Treatment with ARI-509 in high and low doses (HDP, LDP) initiated with the introduction of the galactose diet significantly prevented RCBM thickening at all time points (P < 0.05). In contrast, intervention by withdrawing galactose from the diet or by adding the high dose of ARI-509 had no significant effect (P < 0.05) on RCBM thickening until the 24-month time point (4IN, 166 +/- 10.3 nm; 8IN, 161 +/- 8.2 nm; 4GW, 136 +/- 5.1 nm; 8GW, 163 +/- 9.6 nm). CONCLUSIONS: Both early and late interventions decreased RCBM thickening compared with that in untreated GAL rats. The decreased thickening, however, was not evident until 16 to 20 months after the intervention. Because RCBM thickening is one of the earliest changes in diabetic and galactosemic retinopathy, the findings suggest that RCBM thickening and possibly subsequent retinal lesions are caused by early biochemical alterations induced by the galactose diet that are not readily reversed. The delayed response to therapy is consistent with that observed in the Diabetes Control and Complications Trial. The cumulative evidence indicates that intervention should begin as early after onset of diabetes as possible, and long follow-up periods should be used to evaluate efficacy.